FastQCFastQC Report
Sat 14 Jan 2017
SRR2934280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622185
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152930.9427408094637787No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC33570.20694310451643927No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT28340.17470263872492964Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC27750.1710655689702469No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG26470.1631749769600878No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC24460.15078428169413477Illumina PCR Primer Index 11 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG23950.14764037394008697Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT23850.1470239214392933No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG21910.13506474292389586No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC20920.1289618631660384No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC19620.12094798065572052Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT19470.12002330190453No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC18480.11392042214667254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT26550.044.1525421
CGTTAGG5100.043.676472
CGTTTTT63900.043.0633771
TCGTTAG2800.041.7857131
TAGGGAT13500.041.05
TTAGGGA25950.040.9248544
TTTAGGG52750.040.649293
CTTTAGG16050.040.514022
TCGTTGA451.9292202E-840.00000424
GTTTATT29650.039.688032
TAGGGAG17450.039.197715
TTAGGGG57100.039.0893144
CGTAGGG7500.039.03
CTTAGGG29450.038.8879433
TAGGGGT11600.038.7931065
TAGGGGA29450.038.735145
GTTAGGG20350.038.6977843
TCTAGGG25900.038.6583023
CTAGGGG26950.038.654924
TTGCTAG6450.038.3720931
Produced by FastQC (version 0.11.5)