FastQCFastQC Report
Sat 14 Jan 2017
SRR2934279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262550
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.3340065739970694No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT30220.2393568571541721TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC28150.22296146687259913TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC23860.1889826145499188TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC23730.18795295235832246TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.12094570512058928No Hit
CGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG12810.10146132826422714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG2350.043.0851061
CGTTAGG3600.042.52
CGTTTAT7750.041.516131
GTTAGGG17750.040.943663
TTTAGGG44750.040.8268173
TTAGGGA22300.040.560544
TAGGGAT11050.040.5203635
CGTTTTT21650.040.21941
TTCAGGG20300.040.123153
ATACGGA451.9284926E-840.04
TTAGGGG44250.039.9661034
TAGGGGA21250.039.6000025
TTAGGGT8550.039.4736864
AGGGCGA8550.039.4736866
CGTAGGG4750.039.315793
AGGGGGG39600.039.2613646
CTAGGGA12850.039.221794
TCGAGGG6200.039.193553
TAGGGCG5750.039.1304365
TAGGGGG37400.039.0441175
Produced by FastQC (version 0.11.5)