##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934279.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1262550
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32226367272583	33.0	31.0	34.0	31.0	34.0
2	32.49126688051958	34.0	31.0	34.0	31.0	34.0
3	32.54205536414399	34.0	31.0	34.0	31.0	34.0
4	36.03350362361887	37.0	35.0	37.0	35.0	37.0
5	35.167226644489325	37.0	35.0	37.0	35.0	37.0
6	26.138891133024433	37.0	0.0	37.0	0.0	37.0
7	31.116080155241377	37.0	19.0	37.0	17.0	37.0
8	34.753717476535584	37.0	32.0	37.0	32.0	37.0
9	37.252211001544495	39.0	37.0	39.0	35.0	39.0
10	37.34937784642192	39.0	37.0	39.0	34.0	39.0
11	37.3950528691933	39.0	37.0	39.0	35.0	39.0
12	37.054614074690114	39.0	37.0	39.0	34.0	39.0
13	37.009697041701315	39.0	37.0	39.0	33.0	39.0
14	37.94038731139361	40.0	37.0	41.0	33.0	41.0
15	38.121869232901666	40.0	37.0	41.0	33.0	41.0
16	38.27869628925587	40.0	37.0	41.0	33.0	41.0
17	38.306170052671185	40.0	37.0	41.0	34.0	41.0
18	38.28973426794978	40.0	37.0	41.0	34.0	41.0
19	38.27854263197497	40.0	37.0	41.0	34.0	41.0
20	38.1700019801196	40.0	37.0	41.0	34.0	41.0
21	38.0561783691735	40.0	36.0	41.0	34.0	41.0
22	38.097726030652254	40.0	36.0	41.0	34.0	41.0
23	38.05081462120312	40.0	36.0	41.0	34.0	41.0
24	37.979606352223676	40.0	36.0	41.0	34.0	41.0
25	37.865121381331434	40.0	36.0	41.0	34.0	41.0
26	37.80927171201141	40.0	35.0	41.0	34.0	41.0
27	37.78342085461962	40.0	35.0	41.0	34.0	41.0
28	37.711084709516456	40.0	35.0	41.0	33.0	41.0
29	37.67483505603739	40.0	35.0	41.0	33.0	41.0
30	37.42387469803176	39.0	35.0	41.0	33.0	41.0
31	37.40481881905667	39.0	35.0	41.0	33.0	41.0
32	37.33130331472021	39.0	35.0	41.0	33.0	41.0
33	37.25308779850303	39.0	35.0	41.0	33.0	41.0
34	37.1335741158766	39.0	35.0	41.0	32.0	41.0
35	37.076444497247635	39.0	35.0	41.0	32.0	41.0
36	37.000061779731496	39.0	35.0	41.0	32.0	41.0
37	36.94652647419904	39.0	35.0	41.0	32.0	41.0
38	36.882438715298406	39.0	35.0	41.0	32.0	41.0
39	36.8326941507267	39.0	35.0	41.0	31.0	41.0
40	36.75543701239555	39.0	35.0	41.0	31.0	41.0
41	36.61515979565166	39.0	35.0	41.0	31.0	41.0
42	36.54474278246406	39.0	35.0	41.0	31.0	41.0
43	36.48997267434953	39.0	35.0	41.0	31.0	41.0
44	36.36934458041266	39.0	35.0	40.0	31.0	41.0
45	36.350092273573324	39.0	35.0	40.0	31.0	41.0
46	36.28871252623659	38.0	35.0	40.0	31.0	41.0
47	36.20461130252267	38.0	35.0	40.0	31.0	41.0
48	36.16474199041622	38.0	35.0	40.0	31.0	41.0
49	36.14897469407152	38.0	35.0	40.0	31.0	41.0
50	36.06509207556137	38.0	35.0	40.0	31.0	41.0
51	35.03091758742228	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	14.0
11	10.0
12	16.0
13	11.0
14	11.0
15	42.0
16	51.0
17	139.0
18	235.0
19	455.0
20	791.0
21	1306.0
22	2075.0
23	3215.0
24	4943.0
25	7186.0
26	9688.0
27	11291.0
28	12706.0
29	15339.0
30	19389.0
31	26076.0
32	36880.0
33	61024.0
34	90430.0
35	106014.0
36	120230.0
37	195939.0
38	266545.0
39	270344.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.74579224585165	4.524652489010336	54.388499465367715	16.341055799770306
2	21.43717080511663	4.192388420260584	52.27998891133024	22.090451863292543
3	20.773989148944597	4.091243911132232	52.234446160548096	22.900320779375075
4	18.622232782860085	4.679893865589482	51.721436774781196	24.97643657676924
5	17.774266365688487	7.918815096431825	49.87216347867411	24.434755059205575
6	16.36125301968239	30.79624569323987	39.807611579739415	13.034889707338323
7	82.79854263197497	2.1345689279632487	10.250524731693794	4.8163637083679856
8	84.5694031919528	2.289968714110332	8.424458437289612	4.716169656647262
9	78.96447665438993	4.4273098095124945	11.038533127400894	5.569680408696685
10	42.71910023365411	22.90198407983842	18.763692527028635	15.615223159478834
11	32.082531384895645	22.787057938299473	25.180626509841193	19.949784166963685
12	28.85929270127916	20.747930775018812	29.74915844917033	20.6436180745317
13	26.963050968278484	21.645162567819096	31.632806621519936	19.75897984238248
14	22.111441131044316	22.97231792800285	33.76872203081066	21.14751891014217
15	19.166924082214564	23.07607619500218	36.39309334283791	21.363906379945348
16	24.10336224307948	22.43831927448418	31.54797829788919	21.91034018454715
17	24.04609718426993	23.2374955447309	28.85010494633876	23.86630232466041
18	25.516850817789393	22.090847887212387	31.41388459862976	20.97841669636846
19	25.562155954219634	25.048512930180983	28.110173854500815	21.279157261098568
20	24.668646786265892	27.850698982218525	28.053225614827138	19.427428616688445
21	24.588887568809156	26.011801512811374	30.03413726189062	19.365173656488853
22	23.219674468337885	23.089144984357056	30.889232109619417	22.80194843768564
23	22.137737119322008	23.985347114965744	30.09227357332383	23.784642192388418
24	22.444101223713915	24.88638073739654	29.676052433566984	22.993465605322562
25	23.316304304780008	25.18094332897707	26.935250089105384	24.56750227713754
26	20.635301572214964	25.79501801908835	27.871292226050453	25.69838818264623
27	20.19492297334759	24.763217298324818	30.512454952279118	24.529404776048473
28	18.490594431903688	24.913627183081857	29.030850263355905	27.56492812165855
29	20.359272900083162	25.161775771256583	28.622708011563898	25.85624331709635
30	22.954021622906023	23.874064393489366	28.675299988119285	24.496613995485326
31	22.097659498633718	24.87434161023326	27.877311789632092	25.150687101500928
32	23.019761593600254	25.39875648489169	26.39523187200507	25.186250049502988
33	23.163043047800087	24.230485921349647	26.784760999564373	25.82171003128589
34	19.41491426082135	24.367034968912122	29.629321611025304	26.58872915924122
35	18.78032553166211	26.89841986455982	27.463070769474474	26.85818383430359
36	21.455942338917268	26.947051601916755	26.345887291592412	25.251118767573562
37	19.407627420696212	27.222684250128708	26.45431864084591	26.91536968832918
38	19.985980753237495	25.546314997425846	27.888400459387746	26.57930378994891
39	20.045146726862303	24.223199081224507	28.673715892439905	27.05793829947329
40	22.020434834263988	22.877351392024078	29.456892796324897	25.64532097738703
41	19.014296463506398	24.353174131717555	28.27436537166845	28.358164033107602
42	19.34038255910657	25.36596570432854	29.131044315076633	26.16260742148826
43	19.663934101619738	24.42231990812245	29.162171795176427	26.751574195081385
44	20.63910340184547	23.33665993426003	27.724763375707894	28.299473288186604
45	20.401885073858463	22.701516771613008	26.69462595540771	30.201972199120824
46	19.946457566036987	23.593837867807217	28.741356777949388	27.718347788206408
47	17.304265177616728	24.67767613163835	30.97469407152192	27.043364619223002
48	17.790107322482278	25.172310007524455	29.814502395944714	27.22308027404855
49	20.673557482871967	22.515385529285968	30.05750267316146	26.753554314680606
50	19.073383232347233	22.817234960991644	28.75006930418597	29.35931250247515
51	17.910894618034927	22.974298047602076	27.721595184349134	31.39321215001386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	782.5
2	1291.0
3	5325.0
4	9359.0
5	6049.0
6	2739.0
7	2787.0
8	2835.0
9	2993.0
10	3151.0
11	3192.5
12	3234.0
13	3275.5
14	3317.0
15	3251.0
16	3185.0
17	3243.5
18	3302.0
19	3309.5
20	3317.0
21	3744.0
22	4171.0
23	4292.0
24	4413.0
25	5147.0
26	6844.5
27	7808.0
28	9433.5
29	11059.0
30	13506.5
31	15954.0
32	18907.0
33	21860.0
34	25862.5
35	29865.0
36	31408.0
37	32951.0
38	37036.0
39	41121.0
40	46218.5
41	51316.0
42	58112.5
43	64909.0
44	72066.5
45	79224.0
46	94876.0
47	110528.0
48	126507.0
49	142486.0
50	138820.5
51	135155.0
52	118963.5
53	102772.0
54	92659.5
55	82547.0
56	74687.5
57	66828.0
58	61805.0
59	56782.0
60	51272.5
61	45763.0
62	40079.5
63	34396.0
64	30007.0
65	25618.0
66	22248.0
67	18878.0
68	16373.5
69	13869.0
70	10771.5
71	7674.0
72	6433.0
73	5192.0
74	4345.5
75	2838.5
76	2178.0
77	1553.5
78	929.0
79	769.5
80	610.0
81	386.0
82	162.0
83	131.5
84	101.0
85	63.5
86	26.0
87	19.5
88	13.0
89	8.0
90	3.0
91	2.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1262550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.41567037323403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98451271452514	26.577799573632554
2	10.836803746289531	7.8925894624861455
3	3.9064402101099147	4.267669170723292
4	2.1009912340599532	3.060360169463258
5	1.391055611823449	2.5328111315500053
6	1.0099808175685157	2.206747712151869
7	0.8412917436029306	2.144534197593735
8	0.6899567945953593	2.010019136300619
9	0.5541181831224437	1.8160726593962018
>10	5.5090089263846505	38.870867499788744
>50	0.11844802109487361	2.9305085192101186
>100	0.05260933063087564	3.3073743731489404
>500	0.0027329521098891363	0.7326498746735072
>1k	0.0020497140824168518	1.6499965198810762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4217	0.3340065739970694	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	3022	0.2393568571541721	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2815	0.22296146687259913	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2386	0.1889826145499188	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2373	0.18795295235832246	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1527	0.12094570512058928	No Hit
CGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	1281	0.10146132826422714	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.920478396895172E-5	0.0	0.0	0.25210882737317336	0.0
2	7.920478396895172E-5	0.0	0.0	0.9020632846223912	0.0
3	7.920478396895172E-5	0.0	0.0	1.3315908280860165	0.0
4	7.920478396895172E-5	0.0	0.0	1.8461843095322958	0.0
5	7.920478396895172E-5	0.0	0.0	2.721793196309057	0.0
6	7.920478396895172E-5	0.0	0.0	3.5086135202566235	0.0
7	7.920478396895172E-5	0.0	0.0	4.40045938774702	0.0
8	1.5840956793790345E-4	0.0	0.0	5.748128786978733	0.0
9	1.5840956793790345E-4	0.0	0.0	6.458199675260386	0.0
10	1.5840956793790345E-4	0.0	0.0	7.679379034493683	0.0
11	1.5840956793790345E-4	0.0	0.0	9.855847293176508	0.0
12	1.5840956793790345E-4	0.0	0.0	11.612134172904044	0.0
13	1.5840956793790345E-4	0.0	0.0	12.234842184467942	0.0
14	2.3761435190685517E-4	0.0	0.0	12.497247633757079	0.0
15	2.3761435190685517E-4	0.0	0.0	12.799176270246724	0.0
16	2.3761435190685517E-4	0.0	0.0	13.526513801433607	0.0
17	2.3761435190685517E-4	0.0	0.0	14.495188309373885	0.0
18	2.3761435190685517E-4	0.0	0.0	15.513128192942855	0.0
19	2.3761435190685517E-4	0.0	0.0	16.20632846223912	0.0
20	2.3761435190685517E-4	0.0	0.0	16.989267751772207	0.0
21	2.3761435190685517E-4	0.0	0.0	18.127044473486198	0.0
22	2.3761435190685517E-4	0.0	0.0	19.314165775612846	0.0
23	2.3761435190685517E-4	0.0	0.0	20.514039047958498	0.0
24	2.3761435190685517E-4	0.0	0.0	21.380064155875015	0.0
25	2.3761435190685517E-4	0.0	0.0	22.14573680250287	0.0
26	3.168191358758069E-4	0.0	0.0	22.839649914854856	0.0
27	3.168191358758069E-4	0.0	0.0	23.54290919171518	0.0
28	3.168191358758069E-4	0.0	0.0	24.272226842501286	0.0
29	3.168191358758069E-4	0.0	0.0	25.07686824284187	0.0
30	3.168191358758069E-4	0.0	0.0	26.012989584570906	0.0
31	3.168191358758069E-4	0.0	0.0	26.758068987366837	0.0
32	3.168191358758069E-4	0.0	0.0	27.46362520296226	0.0
33	3.168191358758069E-4	0.0	0.0	28.15421171438755	0.0
34	3.168191358758069E-4	0.0	0.0	28.907053186012437	0.0
35	3.168191358758069E-4	0.0	0.0	29.762385648093144	0.0
36	3.168191358758069E-4	0.0	0.0	30.486792602273177	0.0
37	3.168191358758069E-4	0.0	0.0	31.197893152746428	0.0
38	3.168191358758069E-4	0.0	0.0	32.017266642905234	0.0
39	3.168191358758069E-4	0.0	0.0	33.410082768999246	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	235	0.0	43.085106	1
CGTTAGG	360	0.0	42.5	2
CGTTTAT	775	0.0	41.51613	1
GTTAGGG	1775	0.0	40.94366	3
TTTAGGG	4475	0.0	40.826817	3
TTAGGGA	2230	0.0	40.56054	4
TAGGGAT	1105	0.0	40.520363	5
CGTTTTT	2165	0.0	40.2194	1
TTCAGGG	2030	0.0	40.12315	3
ATACGGA	45	1.9284926E-8	40.0	4
TTAGGGG	4425	0.0	39.966103	4
TAGGGGA	2125	0.0	39.600002	5
TTAGGGT	855	0.0	39.473686	4
AGGGCGA	855	0.0	39.473686	6
CGTAGGG	475	0.0	39.31579	3
AGGGGGG	3960	0.0	39.261364	6
CTAGGGA	1285	0.0	39.22179	4
TCGAGGG	620	0.0	39.19355	3
TAGGGCG	575	0.0	39.130436	5
TAGGGGG	3740	0.0	39.044117	5
>>END_MODULE