FastQCFastQC Report
Sat 14 Jan 2017
SRR2934277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336655
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC160441.2003097283891504No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG139911.0467173653635382No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC118540.8868406582102337No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106350.7956428547381336No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT59170.44267219289943927TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC51660.3864871638530511TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC51140.3825968555835275TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC42440.31750900568957585TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT38800.29027684780291096No Hit
GAATCAGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC38730.2897531524589367No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTC38550.288406507288717No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.2823466040227284No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG35700.2670846254269052No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTTGCCT28250.2113484781039236No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT27640.20678484724929022No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTA27540.2060367110436126No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC19210.14371696511066806No Hit
CTGTCACTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT18330.13713336650070512TruSeq Adapter, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG17870.13369193995458814No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCC17720.13256973564607172No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTT17090.12785647755030283No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT16820.12583650979497327No Hit
GCTGTATCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC16160.12089881083750108TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC15180.11356707602186054TruSeq Adapter, Index 14 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT14590.10915307240836267No Hit
CCTGTATCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC14220.10638496844735552TruSeq Adapter, Index 14 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT13800.10324279638350958No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCA207.033699E-445.00000423
CGAGTGG253.8911472E-545.00000438
CGTGCGA207.033699E-445.00000413
GCGCGAG207.033699E-445.0000041
CCGGCGA502.1827873E-1145.0000048
TAGCGCG207.033699E-445.0000041
TTATCCG406.8157533E-945.0000041
ACGGATA207.033699E-445.00000420
TTGCGAT207.033699E-445.0000041
ACTCGGT207.033699E-445.00000412
CGACTAT253.8911472E-545.00000425
TCGACGG406.8157533E-945.0000042
ATTCGCG453.8562575E-1045.01
CCGCTAG453.8562575E-1045.01
TCCGTAG351.21212E-745.01
CCGTAGG551.8189894E-1244.9999962
ATGCGCG302.1657688E-644.99999612
CGTTTAT21500.044.3720931
CGTTTTT50400.043.7946431
CGCTGAC1250.043.1999971
Produced by FastQC (version 0.11.5)