##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934277.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1336655
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.154221545574586	33.0	31.0	34.0	30.0	34.0
2	32.32077761277218	34.0	31.0	34.0	30.0	34.0
3	32.32359808626759	34.0	31.0	34.0	30.0	34.0
4	35.921388091916015	37.0	35.0	37.0	35.0	37.0
5	35.07120535964778	37.0	35.0	37.0	35.0	37.0
6	25.950319267125774	35.0	0.0	37.0	0.0	37.0
7	31.01587170960345	37.0	19.0	37.0	17.0	37.0
8	34.575460384317566	37.0	32.0	37.0	32.0	37.0
9	37.203767613931795	39.0	37.0	39.0	34.0	39.0
10	37.22138472530309	39.0	37.0	39.0	34.0	39.0
11	37.27363006909038	39.0	37.0	39.0	34.0	39.0
12	37.16935559287924	39.0	37.0	39.0	34.0	39.0
13	37.22493986855247	39.0	37.0	39.0	34.0	39.0
14	38.38100931055508	40.0	38.0	41.0	34.0	41.0
15	38.43568160819359	40.0	38.0	41.0	34.0	41.0
16	38.54471797135386	40.0	38.0	41.0	34.0	41.0
17	38.50050761041555	40.0	38.0	41.0	34.0	41.0
18	38.452120405040944	40.0	38.0	41.0	34.0	41.0
19	38.420754046481704	40.0	37.0	41.0	34.0	41.0
20	38.277899682416184	40.0	37.0	41.0	34.0	41.0
21	38.22384160460253	40.0	37.0	41.0	34.0	41.0
22	38.24006269381404	40.0	37.0	41.0	34.0	41.0
23	38.13069789885947	40.0	37.0	41.0	34.0	41.0
24	38.07116720470129	40.0	37.0	41.0	34.0	41.0
25	37.96967205449424	40.0	36.0	41.0	34.0	41.0
26	37.938928145258124	40.0	36.0	41.0	34.0	41.0
27	37.93834684342632	40.0	36.0	41.0	34.0	41.0
28	37.87036295828018	40.0	36.0	41.0	34.0	41.0
29	37.85542417452521	40.0	36.0	41.0	34.0	41.0
30	37.59437700827813	40.0	36.0	41.0	33.0	41.0
31	37.57164114898759	40.0	36.0	41.0	33.0	41.0
32	37.38336519146676	40.0	36.0	41.0	33.0	41.0
33	37.23096460941679	40.0	36.0	41.0	32.0	41.0
34	37.085935413401366	40.0	36.0	41.0	32.0	41.0
35	37.006334469253474	40.0	36.0	41.0	32.0	41.0
36	36.931613617575216	40.0	36.0	41.0	31.0	41.0
37	36.83518783829784	40.0	36.0	41.0	31.0	41.0
38	36.73067844731812	40.0	35.0	41.0	31.0	41.0
39	36.64668743991531	39.0	35.0	41.0	31.0	41.0
40	36.59906183719808	39.0	35.0	41.0	30.0	41.0
41	36.49727865455185	39.0	35.0	41.0	30.0	41.0
42	36.49431528704116	39.0	35.0	41.0	30.0	41.0
43	36.46096786380929	39.0	35.0	41.0	30.0	41.0
44	36.36343035413027	39.0	35.0	41.0	30.0	41.0
45	36.33503484444378	39.0	35.0	41.0	30.0	41.0
46	36.239145478825876	39.0	35.0	41.0	30.0	41.0
47	36.123738735874255	39.0	35.0	40.0	30.0	41.0
48	36.05845038547718	39.0	35.0	40.0	29.0	41.0
49	36.106670008341716	39.0	35.0	40.0	29.0	41.0
50	36.033397548357655	39.0	35.0	40.0	29.0	41.0
51	35.03786766218658	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	6.0
12	3.0
13	7.0
14	6.0
15	14.0
16	29.0
17	75.0
18	188.0
19	478.0
20	904.0
21	1646.0
22	2771.0
23	4602.0
24	7503.0
25	11898.0
26	15009.0
27	15324.0
28	14526.0
29	15615.0
30	18873.0
31	24761.0
32	34705.0
33	50901.0
34	75280.0
35	101888.0
36	131805.0
37	214712.0
38	294522.0
39	298494.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.3848599676057	3.9375156641018068	54.493867153453955	16.183757214838536
2	22.188747283330404	9.343547886328185	49.22541717945169	19.242287650889722
3	17.1914218702657	9.27120311523916	53.69119181838246	19.846183196112683
4	16.139766805944692	3.378732732081203	58.605848180719775	21.875652281254325
5	19.526504595426644	7.021781985628303	51.8139684510962	21.637744967848846
6	13.480067781140233	33.2568987509866	40.89147910268544	12.371554365187725
7	75.9285679550819	1.0310065050443085	17.962301416595906	5.078124123277885
8	76.69450980245463	4.627222432115992	12.880361798669066	5.797905966760308
9	72.65270395128137	3.2979340218680218	14.314538904953036	9.734823121897572
10	35.69185765960551	27.453082508201444	22.16144031182317	14.693619520369877
11	23.86457238404824	23.99781544227942	31.36127123304069	20.776340940631652
12	20.457186035289585	20.87060610254703	35.500783672675446	23.171424189487936
13	21.340136385230295	22.520321249686717	38.575099782666435	17.564442582416554
14	19.445705885213464	26.24536623137608	34.08082115429935	20.228106729111104
15	15.434723245714116	25.0588222091714	39.197324664928495	20.309129880185985
16	16.979100815094398	26.773625206205043	32.588962746557634	23.65831123214292
17	17.87364727622311	26.60364866027509	32.81677022118647	22.705933842315332
18	17.681151830502262	25.26306339332139	34.406933726354225	22.64885104982213
19	18.10048217378456	27.959495905824618	32.10394604441685	21.83607587597398
20	18.64759418099659	28.577157157232048	34.052391978483605	18.72285668328776
21	19.35495696346477	29.851307929121575	32.69026038880639	18.10347471860727
22	18.119335206167637	24.71378179111289	32.529635545447405	24.63724745727207
23	17.308205931971976	28.597132393923637	31.030295775648913	23.06436589845547
24	19.160366736368022	25.552816545780328	31.06007159663488	24.22674512121677
25	17.543045886934173	30.057868335509163	28.85239646730084	23.546689310255825
26	16.704235573128443	26.683250352559185	32.6273421339089	23.98517194040347
27	19.44383554469927	26.527712835398816	32.317538931137804	21.710912688764118
28	16.813463459157376	24.701886425442616	35.08048075232577	23.404169363074242
29	17.45446656018195	21.85044009112299	36.74949781357194	23.945595535123125
30	19.95758067713808	24.270959970972314	36.681417418855276	19.09004193303433
31	22.407876377973373	24.85877058777321	31.669428536159295	21.06392449809412
32	19.208696335254796	26.725071166456566	32.483849609659934	21.582382888628704
33	19.463810781390862	23.35651308677258	32.181602582566185	24.99807354927038
34	21.641485648877236	22.230119215504374	32.010728273189414	24.117666862428973
35	16.894187355749988	22.163385465957933	33.61151531247779	27.33091186581429
36	19.618749789586694	22.673389917368354	34.11441246993427	23.593447823110676
37	19.686306488959378	23.213319817005885	35.596769547863886	21.50360414617085
38	20.140200724943984	23.747863135962533	32.23359804886077	23.87833809023271
39	20.828486034167383	22.762118871361718	34.30099763963027	22.108397454840627
40	20.53027894258429	22.054756088893544	33.80146709509933	23.613497873422833
41	20.957913597749606	23.19476603910508	28.14323815793903	27.704082205206284
42	19.045078947073105	22.089918490560393	31.01578193325877	27.849220629107734
43	20.015561233078095	21.816025825661818	31.17640677661775	26.992006164642333
44	20.015112351354688	22.237076882217178	31.540225413438773	26.207585352989366
45	20.28257104488443	20.924845977458656	30.993861542432416	27.7987214352245
46	23.366912180031495	22.032760884446624	31.87494155185893	22.72538538366295
47	17.475788442043758	21.707920143941408	37.040672424821665	23.775618989193173
48	18.92762156278172	22.664412282900226	32.97140997490003	25.436556179418023
49	20.176560144539916	19.450269516068094	36.10744732185942	24.265723017532572
50	20.94310050087719	18.642880922900822	33.30672462228473	27.107293953937255
51	18.829166838114546	18.854902723589856	30.46799660346163	31.84793383483397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	392.0
2	612.0
3	9930.0
4	19248.0
5	12354.0
6	5460.0
7	5581.0
8	5702.0
9	6270.5
10	6839.0
11	7459.0
12	8079.0
13	8217.0
14	8355.0
15	8164.0
16	7973.0
17	7738.5
18	7504.0
19	7220.5
20	6937.0
21	6685.5
22	6434.0
23	6351.0
24	6268.0
25	6749.5
26	8453.5
27	9676.0
28	11325.0
29	12974.0
30	14939.0
31	16904.0
32	19317.0
33	21730.0
34	26030.0
35	30330.0
36	34565.0
37	38800.0
38	44620.0
39	50440.0
40	57957.0
41	65474.0
42	75552.5
43	85631.0
44	101766.0
45	117901.0
46	166823.5
47	215746.0
48	202549.5
49	189353.0
50	170589.5
51	151826.0
52	119184.0
53	86542.0
54	69557.5
55	52573.0
56	43832.0
57	35091.0
58	28875.5
59	22660.0
60	18944.5
61	15229.0
62	12239.5
63	9250.0
64	7319.0
65	5388.0
66	4115.5
67	2843.0
68	2150.5
69	1458.0
70	1187.5
71	917.0
72	729.5
73	542.0
74	434.5
75	206.0
76	85.0
77	69.5
78	54.0
79	58.5
80	63.0
81	43.5
82	24.0
83	14.5
84	5.0
85	4.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1336655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.89009109792438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87922842678309	30.209283806155252
2	12.20133311541419	9.978272452108158
3	4.353704356158237	5.340701032102216
4	2.2380764863079703	3.660606056370217
5	1.3542336523228118	2.7687368705677318
6	0.9806171698127123	2.4058515243498353
7	0.6946845478446405	1.9884000111981437
8	0.577840144342474	1.8902348913761229
9	0.4648465267288614	1.7106855142047201
>10	3.0800080391746065	21.946410313763284
>50	0.10731629803383504	3.071436626612454
>100	0.05807785575840314	4.267870278655798
>500	0.004244892096169409	1.1701256170707546
>1k	0.004437841736904382	4.169484760659306
>5k	5.788489222049195E-4	1.2778993068366942
>10k+	7.717985629398926E-4	4.14400093796941
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	16044	1.2003097283891504	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG	13991	1.0467173653635382	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	11854	0.8868406582102337	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10635	0.7956428547381336	No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	5917	0.44267219289943927	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	5166	0.3864871638530511	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	5114	0.3825968555835275	TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	4244	0.31750900568957585	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT	3880	0.29027684780291096	No Hit
GAATCAGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	3873	0.2897531524589367	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTC	3855	0.288406507288717	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3774	0.2823466040227284	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG	3570	0.2670846254269052	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTTGCCT	2825	0.2113484781039236	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	2764	0.20678484724929022	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTA	2754	0.2060367110436126	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	1921	0.14371696511066806	No Hit
CTGTCACTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	1833	0.13713336650070512	TruSeq Adapter, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG	1787	0.13369193995458814	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCC	1772	0.13256973564607172	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTT	1709	0.12785647755030283	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT	1682	0.12583650979497327	No Hit
GCTGTATCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	1616	0.12089881083750108	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	1518	0.11356707602186054	TruSeq Adapter, Index 14 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	1459	0.10915307240836267	No Hit
CCTGTATCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	1422	0.10638496844735552	TruSeq Adapter, Index 14 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	1380	0.10324279638350958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46324593855557344	0.0
2	0.0	0.0	0.0	1.6290665878629864	0.0
3	0.0	0.0	0.0	2.3923151448952797	0.0
4	0.0	0.0	0.0	3.4065633989324096	0.0
5	0.0	0.0	0.0	5.6535156790645305	0.0
6	0.0	0.0	0.0	6.856069816070714	0.0
7	0.0	0.0	0.0	8.472717342919452	0.0
8	0.0	0.0	0.0	10.641863457661103	0.0
9	0.0	0.0	0.0	11.532145542417453	0.0
10	0.0	0.0	0.0	14.144637172643652	0.0
11	0.0	0.0	0.0	17.139501217591675	0.0
12	0.0	0.0	0.0	21.0530765231118	0.0
13	0.0	0.0	0.0	22.073459494035482	0.0
14	0.0	0.0	0.0	22.463687338916923	0.0
15	0.0	0.0	0.0	23.05381717795542	0.0
16	0.0	0.0	0.0	23.94709180753448	0.0
17	0.0	0.0	0.0	25.248474737310676	0.0
18	7.481362056776057E-5	0.0	0.0	26.655120431225708	0.0
19	7.481362056776057E-5	0.0	0.0	27.954184138764305	0.0
20	7.481362056776057E-5	0.0	0.0	29.025141117191797	0.0
21	7.481362056776057E-5	0.0	0.0	30.495752456692266	0.0
22	7.481362056776057E-5	0.0	0.0	32.1374625464312	0.0
23	7.481362056776057E-5	0.0	0.0	33.708024883010204	0.0
24	7.481362056776057E-5	0.0	0.0	34.85057849632104	0.0
25	7.481362056776057E-5	0.0	0.0	35.781185122563414	0.0
26	7.481362056776057E-5	0.0	0.0	36.643112845124584	0.0
27	1.4962724113552113E-4	0.0	0.0	37.48828231667857	0.0
28	1.4962724113552113E-4	0.0	0.0	38.37587111109448	0.0
29	1.4962724113552113E-4	0.0	0.0	39.31253764060285	0.0
30	1.4962724113552113E-4	0.0	0.0	40.341449364271256	0.0
31	2.244408617032817E-4	0.0	0.0	41.279238098088136	0.0
32	2.244408617032817E-4	0.0	0.0	42.10533009639735	0.0
33	2.244408617032817E-4	0.0	0.0	42.91773120214266	0.0
34	2.244408617032817E-4	0.0	0.0	43.725793117895044	0.0
35	2.244408617032817E-4	0.0	0.0	44.61218489438187	0.0
36	2.244408617032817E-4	0.0	0.0	45.43865096079392	0.0
37	2.244408617032817E-4	0.0	0.0	46.22307177244689	0.0
38	2.244408617032817E-4	0.0	0.0	47.03958762732343	0.0
39	2.244408617032817E-4	0.0	0.0	47.930318593803186	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCA	20	7.033699E-4	45.000004	23
CGAGTGG	25	3.8911472E-5	45.000004	38
CGTGCGA	20	7.033699E-4	45.000004	13
GCGCGAG	20	7.033699E-4	45.000004	1
CCGGCGA	50	2.1827873E-11	45.000004	8
TAGCGCG	20	7.033699E-4	45.000004	1
TTATCCG	40	6.8157533E-9	45.000004	1
ACGGATA	20	7.033699E-4	45.000004	20
TTGCGAT	20	7.033699E-4	45.000004	1
ACTCGGT	20	7.033699E-4	45.000004	12
CGACTAT	25	3.8911472E-5	45.000004	25
TCGACGG	40	6.8157533E-9	45.000004	2
ATTCGCG	45	3.8562575E-10	45.0	1
CCGCTAG	45	3.8562575E-10	45.0	1
TCCGTAG	35	1.21212E-7	45.0	1
CCGTAGG	55	1.8189894E-12	44.999996	2
ATGCGCG	30	2.1657688E-6	44.999996	12
CGTTTAT	2150	0.0	44.372093	1
CGTTTTT	5040	0.0	43.794643	1
CGCTGAC	125	0.0	43.199997	1
>>END_MODULE