SRR2934274.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934274.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1060383
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	4138	0.3902363579951772	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	4093	0.38599260833114074	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3913	0.36901760967499475	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3869	0.364868165559048	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	2946	0.2778241446722552	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1774	0.16729804231112722	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1645	0.15513262660755595	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.1358942947972572	No Hit
TCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1396	0.13165054513322072	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	1332	0.12561499005547996	No Hit
GCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1268	0.11957943497773918	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1216	0.11467554647707479	No Hit
CTGTCACTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1145	0.1079798525627061	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1088	0.10260443632159325	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGACG	90	0.0	45.000004	6
TCACCCG	25	3.8906008E-5	45.0	28
CACGGGT	25	3.8906008E-5	45.0	4
ACGTTAG	25	3.8906008E-5	45.0	1
GCGCGAC	35	1.2118107E-7	45.0	9
GGCGTAC	20	7.0330396E-4	45.0	9
AGGCGAT	70	0.0	45.0	6
ACGATTA	20	7.0330396E-4	45.0	10
CCCGTAG	20	7.0330396E-4	45.0	1
TGCGATT	20	7.0330396E-4	45.0	44
AGTTGCG	20	7.0330396E-4	45.0	1
CTTGCGA	25	3.8906008E-5	45.0	20
CCGAGTA	20	7.0330396E-4	45.0	16
TCGCTAG	35	1.2118107E-7	45.0	1
GGAACGT	20	7.0330396E-4	45.0	8
CGAATAT	25	3.8906008E-5	45.0	14
GACGATT	25	3.8906008E-5	45.0	9
TATCGCT	20	7.0330396E-4	45.0	3
GCGTACC	20	7.0330396E-4	45.0	10
AACCGTG	30	2.1653432E-6	44.999996	38