##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934274.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1060383
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19638375945295	33.0	31.0	34.0	30.0	34.0
2	32.363983579517964	34.0	31.0	34.0	30.0	34.0
3	32.40852503293621	34.0	31.0	34.0	30.0	34.0
4	35.94140984908283	37.0	35.0	37.0	35.0	37.0
5	35.0595954480598	37.0	35.0	37.0	35.0	37.0
6	25.972621213278597	36.0	0.0	37.0	0.0	37.0
7	31.04985368494214	37.0	19.0	37.0	17.0	37.0
8	34.7343176946443	37.0	32.0	37.0	32.0	37.0
9	37.2536272271434	39.0	37.0	39.0	35.0	39.0
10	37.15869643326987	39.0	37.0	39.0	34.0	39.0
11	37.20373016164914	39.0	37.0	39.0	34.0	39.0
12	37.13321318806506	39.0	37.0	39.0	34.0	39.0
13	37.2110925957885	39.0	37.0	39.0	34.0	39.0
14	38.35807062165274	40.0	38.0	41.0	34.0	41.0
15	38.473612836116764	40.0	38.0	41.0	34.0	41.0
16	38.51800905899095	40.0	38.0	41.0	34.0	41.0
17	38.52190765034898	40.0	38.0	41.0	34.0	41.0
18	38.499734529882126	40.0	38.0	41.0	34.0	41.0
19	38.50842761530504	40.0	38.0	41.0	34.0	41.0
20	38.4229207748521	40.0	38.0	41.0	34.0	41.0
21	38.32488638539094	40.0	37.0	41.0	34.0	41.0
22	38.32016827882001	40.0	37.0	41.0	34.0	41.0
23	38.23081565811598	40.0	37.0	41.0	34.0	41.0
24	38.17461803895385	40.0	37.0	41.0	34.0	41.0
25	38.055485612274055	40.0	37.0	41.0	34.0	41.0
26	38.032903205728495	40.0	37.0	41.0	34.0	41.0
27	38.03338982235664	40.0	37.0	41.0	34.0	41.0
28	37.9918840645314	40.0	37.0	41.0	34.0	41.0
29	37.980987058449635	40.0	37.0	41.0	34.0	41.0
30	37.732152439260155	40.0	37.0	41.0	33.0	41.0
31	37.73798712352046	40.0	37.0	41.0	33.0	41.0
32	37.63597021076347	40.0	36.0	41.0	33.0	41.0
33	37.57151425475512	40.0	37.0	41.0	33.0	41.0
34	37.53469642572542	40.0	37.0	41.0	33.0	41.0
35	37.48516243659131	40.0	37.0	41.0	33.0	41.0
36	37.440480467906404	40.0	37.0	41.0	33.0	41.0
37	37.360648935337515	40.0	36.0	41.0	33.0	41.0
38	37.19874988565452	40.0	36.0	41.0	32.0	41.0
39	37.1461952898151	40.0	36.0	41.0	32.0	41.0
40	37.138650845967916	39.0	36.0	41.0	32.0	41.0
41	37.064358821293816	39.0	36.0	41.0	32.0	41.0
42	37.01650252786022	39.0	36.0	41.0	31.0	41.0
43	36.98594281500175	39.0	36.0	41.0	31.0	41.0
44	36.86628699253006	39.0	35.0	41.0	31.0	41.0
45	36.84109892369078	39.0	35.0	41.0	31.0	41.0
46	36.76696156011554	39.0	35.0	41.0	31.0	41.0
47	36.69369463674917	39.0	35.0	40.0	31.0	41.0
48	36.65869690479761	39.0	35.0	40.0	31.0	41.0
49	36.65651561747029	39.0	35.0	40.0	31.0	41.0
50	36.56463749418842	39.0	35.0	40.0	31.0	41.0
51	35.57934444441301	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	4.0
12	2.0
13	5.0
14	4.0
15	12.0
16	26.0
17	51.0
18	119.0
19	234.0
20	436.0
21	749.0
22	1340.0
23	2286.0
24	3756.0
25	5808.0
26	7628.0
27	8513.0
28	9635.0
29	11453.0
30	14760.0
31	19462.0
32	26409.0
33	39808.0
34	59135.0
35	81955.0
36	107980.0
37	178773.0
38	244532.0
39	235412.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.170008383763225	4.219230221533163	59.07431560106112	14.536445793642486
2	20.657253086856354	3.6992294293665595	53.85384337545962	21.789674108317467
3	18.666180050038523	3.7060194288290176	55.3582997841346	22.26950073699786
4	17.58987082969078	3.937068021648782	54.67854539350404	23.794515755156393
5	16.622861739579	7.444574271748981	53.288481614661876	22.644082374010146
6	14.84642813021333	30.617333548349983	41.94663626255796	12.589602058878727
7	82.50103971866768	1.260676566863105	11.265080635958894	4.973203078510312
8	84.49088678336035	1.2608651779592845	8.865381659268397	5.3828663794119676
9	79.43837273890661	4.154913837735988	11.202178835383064	5.204534587974345
10	36.12166547370148	29.78395541988131	19.858673705632775	14.235705400784434
11	22.675014593783565	26.364624857244976	29.86741582994069	21.092944719030765
12	20.58888156449132	22.199054492574852	34.912668347191534	22.299395595742293
13	21.56579273715252	24.74596442983337	35.360619700617605	18.327623132396504
14	19.569061367449308	26.76202843689497	33.24147972949397	20.427430466161756
15	17.11032711765466	26.317094861007767	35.865531605089856	20.707046416247714
16	18.22813077916187	27.109450076057424	32.23325911486698	22.42916002991372
17	18.609313804540434	27.190175625222206	30.142127891525988	24.05838267871137
18	19.097345015904633	25.358290353579793	33.59097609071439	21.953388539801185
19	19.796526349441663	28.11757638513631	31.344052101929208	20.741845163492815
20	20.582185870576954	28.507624132035314	30.728708400643917	20.18148159674382
21	20.835113350553527	28.41416733387842	31.963073719589996	18.787645595978056
22	20.20581242815096	26.062752797809846	29.876846384749662	23.85458838928953
23	18.78245879083312	27.395950331153934	29.059122977263875	24.76246790074907
24	20.02031341505852	26.8651987065051	29.90928749329252	23.205200385143858
25	18.64005741321768	28.336742478896777	27.94094209356431	25.08225801432124
26	17.701528598628986	28.430765110342204	29.24622518467384	24.621481106354967
27	18.735777544528723	26.58313081216881	29.913248326312285	24.76784331699018
28	16.300336765112228	25.9350630856964	30.663071739173485	27.101528410017888
29	16.751117284980992	24.45446598068811	30.774446591467424	28.019970142863475
30	19.717121077950136	25.68109824469083	29.54026988361752	25.06151079374151
31	19.749373575396813	27.872287654555006	26.861237873485333	25.517100896562845
32	17.860904974900578	28.868720075670772	27.58418420514097	25.68619074428768
33	18.800093928325897	25.889041978228622	28.98452728872492	26.326336804720558
34	17.93861274652649	24.999646354194663	29.60345460083762	27.458286298441227
35	16.764980200550177	25.027655101977302	29.470389472483056	28.736975224989465
36	18.11468120480996	24.816127757612108	30.014438179412533	27.054752858165397
37	17.654753046776495	26.262114726471474	30.189186360022745	25.893945866729283
38	16.867301720227506	28.199150684233903	30.28905593544974	24.644491660088853
39	18.729647683902893	26.214584730234264	32.72534546479904	22.330422121063805
40	21.635295926094628	23.583648549627824	32.06058565631474	22.7204698679628
41	19.28265541790089	23.0757188676167	31.289354884037184	26.352270830445224
42	18.74520809933769	23.161348305282146	31.68977624122605	26.403667354154116
43	18.58941533389351	23.338076902402243	31.714389989277457	26.358117774426788
44	18.134956897649246	25.007662325782288	28.18160985228922	28.67577092427925
45	20.26918575646724	23.256219686660387	27.112467853596296	29.362126703276083
46	21.07870458126922	23.219912050645853	29.272253515946595	26.429129852138335
47	17.149935447852332	23.730953815743934	31.83698720179407	27.282123534609664
48	17.057327399628246	23.60364132582284	31.962602191849548	27.376429082699367
49	19.257853058753298	21.673301061974776	33.31022847405136	25.75861740522057
50	19.896395924868656	21.000053754162412	31.107816703964513	27.99573361700442
51	18.18418439375207	20.695541139380772	29.70775653702483	31.41251792984233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	294.0
2	491.0
3	3983.5
4	7476.0
5	4862.0
6	2248.0
7	2351.0
8	2454.0
9	2816.0
10	3178.0
11	3453.0
12	3728.0
13	3889.5
14	4051.0
15	3987.0
16	3923.0
17	3877.5
18	3832.0
19	3693.5
20	3555.0
21	3523.5
22	3492.0
23	3981.0
24	4470.0
25	4985.0
26	7045.5
27	8591.0
28	9451.0
29	10311.0
30	12787.5
31	15264.0
32	17882.5
33	20501.0
34	23691.5
35	26882.0
36	30586.5
37	34291.0
38	38048.5
39	41806.0
40	47984.5
41	54163.0
42	62728.5
43	71294.0
44	81793.0
45	92292.0
46	109736.5
47	127181.0
48	141915.0
49	156649.0
50	142698.5
51	128748.0
52	105272.5
53	81797.0
54	66876.0
55	51955.0
56	42796.0
57	33637.0
58	28125.5
59	22614.0
60	18191.5
61	13769.0
62	11474.5
63	9180.0
64	7075.5
65	4971.0
66	3846.0
67	2721.0
68	2022.0
69	1323.0
70	1089.0
71	855.0
72	659.0
73	463.0
74	396.5
75	238.0
76	146.0
77	100.5
78	55.0
79	48.0
80	41.0
81	32.5
82	24.0
83	23.5
84	23.0
85	15.0
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1060383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.69902227885585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87140218882892	31.115376255522715
2	12.17214873101187	10.394776996940456
3	4.517335520559867	5.78657430100358
4	2.388379077643614	4.079258057866313
5	1.4889974889410058	3.178936847672621
6	1.0584135866083233	2.711593518889951
7	0.7921471341767473	2.3676735691241326
8	0.6170753489310921	2.1078811257393153
9	0.5093842621064231	1.9575188960562637
>10	3.4244004653943527	25.441580075403873
>50	0.1028856724889468	3.0465559228535866
>100	0.05132570398198652	3.956967847704196
>500	0.0029393574536633353	0.8369431995416877
>1k	0.0031654618731758997	3.0183633856813152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	4138	0.3902363579951772	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	4093	0.38599260833114074	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3913	0.36901760967499475	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3869	0.364868165559048	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	2946	0.2778241446722552	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1774	0.16729804231112722	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1645	0.15513262660755595	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.1358942947972572	No Hit
TCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1396	0.13165054513322072	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	1332	0.12561499005547996	No Hit
GCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1268	0.11957943497773918	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1216	0.11467554647707479	No Hit
CTGTCACTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1145	0.1079798525627061	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1088	0.10260443632159325	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.82916644269099E-4	0.0	0.0	0.4162646892679343	0.0
2	2.82916644269099E-4	0.0	0.0	1.542367239007038	0.0
3	2.82916644269099E-4	0.0	0.0	2.3067137062740537	0.0
4	2.82916644269099E-4	0.0	0.0	3.1446185010510352	0.0
5	2.82916644269099E-4	0.0	0.0	4.530910057969621	0.0
6	2.82916644269099E-4	0.0	0.0	5.552333449329158	0.0
7	2.82916644269099E-4	0.0	0.0	6.779908768812778	0.0
8	2.82916644269099E-4	0.0	0.0	8.790220137440906	0.0
9	3.772221923587987E-4	0.0	0.0	9.653870346846375	0.0
10	3.772221923587987E-4	0.0	0.0	11.214627167730905	0.0
11	3.772221923587987E-4	0.0	0.0	14.335386365115246	0.0
12	3.772221923587987E-4	0.0	0.0	16.983391849925923	0.0
13	3.772221923587987E-4	0.0	0.0	17.884669973019182	0.0
14	3.772221923587987E-4	0.0	0.0	18.167586617288283	0.0
15	4.715277404484983E-4	0.0	0.0	18.53613269922283	0.0
16	4.715277404484983E-4	0.0	0.0	19.464099292425473	0.0
17	4.715277404484983E-4	0.0	0.0	20.961577090541812	0.0
18	4.715277404484983E-4	0.0	0.0	22.52601182780184	0.0
19	4.715277404484983E-4	0.0	0.0	23.643815489309052	0.0
20	5.65833288538198E-4	0.0	0.0	24.70305540545256	0.0
21	5.65833288538198E-4	0.0	0.0	26.284182224724464	0.0
22	5.65833288538198E-4	0.0	0.0	27.975363618617045	0.0
23	5.65833288538198E-4	0.0	0.0	29.722373896978734	0.0
24	6.601388366278976E-4	0.0	0.0	30.96664129847423	0.0
25	6.601388366278976E-4	0.0	0.0	32.0014560776625	0.0
26	6.601388366278976E-4	0.0	0.0	32.952810446791396	0.0
27	6.601388366278976E-4	0.0	0.0	33.88068273444595	0.0
28	6.601388366278976E-4	0.0	0.0	34.85382168518356	0.0
29	6.601388366278976E-4	0.0	0.0	35.84742493985664	0.0
30	7.544443847175974E-4	0.0	0.0	36.90732499483677	0.0
31	8.48749932807297E-4	0.0	0.0	37.91912922029116	0.0
32	8.48749932807297E-4	0.0	0.0	38.82003012119206	0.0
33	8.48749932807297E-4	0.0	0.0	39.71678157797702	0.0
34	8.48749932807297E-4	0.0	0.0	40.59731248049054	0.0
35	8.48749932807297E-4	0.0	0.0	41.565170320535124	0.0
36	8.48749932807297E-4	0.0	0.0	42.45796094430031	0.0
37	8.48749932807297E-4	0.0	0.0	43.32472323679274	0.0
38	8.48749932807297E-4	0.0	0.0	44.18214928002429	0.0
39	8.48749932807297E-4	0.0	0.0	45.104646151437734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGACG	90	0.0	45.000004	6
TCACCCG	25	3.8906008E-5	45.0	28
CACGGGT	25	3.8906008E-5	45.0	4
ACGTTAG	25	3.8906008E-5	45.0	1
GCGCGAC	35	1.2118107E-7	45.0	9
GGCGTAC	20	7.0330396E-4	45.0	9
AGGCGAT	70	0.0	45.0	6
ACGATTA	20	7.0330396E-4	45.0	10
CCCGTAG	20	7.0330396E-4	45.0	1
TGCGATT	20	7.0330396E-4	45.0	44
AGTTGCG	20	7.0330396E-4	45.0	1
CTTGCGA	25	3.8906008E-5	45.0	20
CCGAGTA	20	7.0330396E-4	45.0	16
TCGCTAG	35	1.2118107E-7	45.0	1
GGAACGT	20	7.0330396E-4	45.0	8
CGAATAT	25	3.8906008E-5	45.0	14
GACGATT	25	3.8906008E-5	45.0	9
TATCGCT	20	7.0330396E-4	45.0	3
GCGTACC	20	7.0330396E-4	45.0	10
AACCGTG	30	2.1653432E-6	44.999996	38
>>END_MODULE