SRR2934271.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728975
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	3436	0.4713467540039096	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	3219	0.4415789293185637	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2552	0.3500805926129154	No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2412	0.3308755444288213	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2369	0.3249768510579924	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1002	0.13745327343187352	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	959	0.13155458006104462	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	944	0.12949689632703454	No Hit
CTGTCACTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	895	0.12277512946260159	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	820	0.1124867107925512	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	750	0.10288418670050413	No Hit
GCTGTATCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	731	0.10027778730409137	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	20	7.031586E-4	45.000004	26
ACGTTAT	20	7.031586E-4	45.000004	15
TCGATCC	20	7.031586E-4	45.000004	44
GCGTTAC	20	7.031586E-4	45.000004	25
AGTATAC	20	7.031586E-4	45.000004	14
CGTTACC	20	7.031586E-4	45.000004	19
AATTCGC	20	7.031586E-4	45.000004	13
ACTCGAT	20	7.031586E-4	45.000004	42
ACCACGC	20	7.031586E-4	45.000004	26
CGTAAGA	20	7.031586E-4	45.000004	39
CGACTCG	20	7.031586E-4	45.000004	13
CGTCAAC	20	7.031586E-4	45.000004	43
GCCCGTA	20	7.031586E-4	45.000004	30
TTTCGTA	25	3.889397E-5	45.0	23
GCGATAT	25	3.889397E-5	45.0	9
CGTAAAT	25	3.889397E-5	45.0	26
GCGTGTA	25	3.889397E-5	45.0	17
GGACCGT	35	1.2111013E-7	45.0	8
ACACGAC	30	2.1644046E-6	44.999996	26
ACGGACT	30	2.1644046E-6	44.999996	42