##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728975
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32649953702116	33.0	31.0	34.0	31.0	34.0
2	32.49541067937858	34.0	31.0	34.0	31.0	34.0
3	32.55236324976851	34.0	31.0	34.0	31.0	34.0
4	36.034206934394184	37.0	35.0	37.0	35.0	37.0
5	35.17221578243424	37.0	35.0	37.0	35.0	37.0
6	26.137088377516374	37.0	0.0	37.0	0.0	37.0
7	31.12448986590761	37.0	19.0	37.0	17.0	37.0
8	34.73020473953154	37.0	32.0	37.0	32.0	37.0
9	37.24551184882883	39.0	37.0	39.0	34.0	39.0
10	37.35671868033883	39.0	37.0	39.0	34.0	39.0
11	37.42303919887513	39.0	37.0	39.0	35.0	39.0
12	37.16091086799959	39.0	37.0	39.0	34.0	39.0
13	37.139542508316474	39.0	37.0	39.0	33.0	39.0
14	38.29463150313797	40.0	37.0	41.0	34.0	41.0
15	38.42103501491821	40.0	38.0	41.0	34.0	41.0
16	38.49177269453685	40.0	38.0	41.0	35.0	41.0
17	38.47432079289413	40.0	38.0	41.0	34.0	41.0
18	38.45281799787373	40.0	37.0	41.0	34.0	41.0
19	38.45846428203985	40.0	37.0	41.0	34.0	41.0
20	38.38772660242121	40.0	37.0	41.0	34.0	41.0
21	38.283650330944134	40.0	37.0	41.0	34.0	41.0
22	38.28297266710106	40.0	37.0	41.0	34.0	41.0
23	38.24693302239446	40.0	37.0	41.0	34.0	41.0
24	38.204562570732875	40.0	37.0	41.0	34.0	41.0
25	38.09654103364313	40.0	36.0	41.0	34.0	41.0
26	38.05604993312528	40.0	36.0	41.0	34.0	41.0
27	38.05302513803628	40.0	36.0	41.0	34.0	41.0
28	37.97179464316335	40.0	36.0	41.0	34.0	41.0
29	37.940067903563225	40.0	36.0	41.0	34.0	41.0
30	37.72356116464899	40.0	36.0	41.0	33.0	41.0
31	37.7300936246099	40.0	36.0	41.0	33.0	41.0
32	37.67508213587572	40.0	36.0	41.0	33.0	41.0
33	37.58020782605713	40.0	36.0	41.0	33.0	41.0
34	37.444703865015946	40.0	36.0	41.0	33.0	41.0
35	37.421100860797694	40.0	35.0	41.0	33.0	41.0
36	37.37136801673583	40.0	35.0	41.0	33.0	41.0
37	37.271784354744675	39.0	35.0	41.0	33.0	41.0
38	37.18832607428238	39.0	35.0	41.0	33.0	41.0
39	37.16937069172468	39.0	35.0	41.0	33.0	41.0
40	37.076296169278784	39.0	35.0	41.0	32.0	41.0
41	37.031032614287184	39.0	35.0	41.0	32.0	41.0
42	36.999960218114474	39.0	35.0	41.0	32.0	41.0
43	36.873681539147434	39.0	35.0	41.0	32.0	41.0
44	36.72873280976714	39.0	35.0	41.0	31.0	41.0
45	36.7527034534792	39.0	35.0	41.0	31.0	41.0
46	36.71789567543468	39.0	35.0	41.0	31.0	41.0
47	36.67451147158682	39.0	35.0	41.0	31.0	41.0
48	36.657428581227066	39.0	35.0	41.0	31.0	41.0
49	36.62198703659248	39.0	35.0	40.0	31.0	41.0
50	36.5261209232141	39.0	35.0	40.0	31.0	41.0
51	35.57041050790494	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	8.0
11	3.0
12	10.0
13	5.0
14	9.0
15	9.0
16	24.0
17	53.0
18	74.0
19	197.0
20	313.0
21	533.0
22	888.0
23	1311.0
24	2084.0
25	3213.0
26	4407.0
27	5616.0
28	6436.0
29	7661.0
30	9930.0
31	13116.0
32	18237.0
33	31148.0
34	46949.0
35	64095.0
36	68281.0
37	114010.0
38	161257.0
39	168990.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.568469426249184	4.3728522926026265	52.931719194759765	18.12695908638842
2	20.3469254775541	4.791659521931479	52.91141671525087	21.949998285263554
3	20.06323948009191	4.9457114441510335	51.988065434342744	23.002983641414314
4	19.758839466374017	4.719366233409924	51.17212524434994	24.349669055866112
5	17.661511025755342	8.041702390342605	49.10826845913783	25.188518124764226
6	15.643609177269454	31.454302273740524	39.4695291333722	13.43255941561782
7	81.72721972632806	2.3126993381117322	10.855104770396789	5.104976165163414
8	81.94341369731472	3.144689461229809	9.001131726053705	5.910765115401763
9	76.73527898761961	4.901677012243218	11.782708597688535	6.580335402448643
10	39.52892760382729	25.88154600637882	19.196680270242464	15.392846119551425
11	28.972186974861962	25.22637950547001	26.290201995953222	19.511231523714805
12	28.563942522034363	20.097259851160874	31.956239925923384	19.382557700881375
13	24.206591446894613	23.537844233341335	31.655406564011113	20.60015775575294
14	19.292431153331734	26.4485064645564	31.651702733289895	22.607359648821976
15	17.696354470317914	24.36517027332899	36.5297849720498	21.408690284303304
16	19.833190438629583	23.83044686031757	33.25354093075894	23.082821770293908
17	19.095030693782366	24.426214890771288	30.27524949415275	26.203504921293597
18	20.949826811619054	24.134298158373056	32.41194828354882	22.50392674645907
19	22.32449672485339	25.685654514901056	30.354401728454338	21.635447031791212
20	25.258342192804967	25.735724819095307	29.95479954730958	19.05113344079015
21	24.265441201687302	26.53300867656641	29.292499742789534	19.909050378956756
22	21.43914400356665	24.758050687609316	29.32418807229329	24.478617236530745
23	21.07123015192565	25.799101478102816	28.535683665420624	24.593984704550913
24	22.647964607839775	23.194348228677253	30.55920984944614	23.598477314036835
25	20.38382660585068	24.644329366576358	28.3065948763675	26.66524915120546
26	18.38362083747728	27.361294968963268	29.527761583044686	24.72732261051476
27	19.570355636338697	26.216125381528858	30.487053739840185	23.72646524229226
28	16.971912617030764	25.476456668610037	33.31979834699407	24.231832367365136
29	18.247813711032617	24.743509722555643	32.41455468294523	24.59412188346651
30	20.732946946054394	25.070818615178847	29.876881923248394	24.319352515518364
31	21.36108920058987	27.85445317054769	27.586817106210777	23.197640522651668
32	22.515586954285126	27.76926506395967	26.639596693988132	23.07555128776707
33	23.58379917006756	25.40649542165369	26.237525292362562	24.772180115916186
34	20.413457251620425	24.584519359374465	29.819815494358515	25.18220789464659
35	20.052127987928255	24.593298809972907	29.12431839226311	26.230254809835728
36	21.49826811619054	25.535717960149523	28.682602284028945	24.283411639630987
37	19.74059467059913	27.065674405843822	30.392949003738124	22.800781919818924
38	20.094790630680066	28.435680235947736	27.687917967008467	23.78161116636373
39	21.574128056517715	26.085256696045818	27.812887959120687	24.527727288315788
40	22.211186940567234	25.423779965019378	28.66092801536404	23.704105079049352
41	20.837888816488906	24.892486024897973	27.997942316265988	26.271682842347133
42	23.94910662231215	22.793785795123288	27.585719674885972	25.67138790767859
43	22.727528378888167	23.060324428135395	28.672862581021295	25.539284611955143
44	19.96131554580061	23.875578723550188	28.719640591241124	27.443465139408076
45	20.098905998148084	23.91892726088	27.645118145341062	28.337048595630854
46	21.14928495490243	24.708940635824273	29.38001989094276	24.761754518330534
47	18.785006344524845	24.56476559552797	31.676943653760414	24.973284406186767
48	19.6936794814637	23.104770396790013	31.58750300078878	25.614047120957512
49	21.346959772283	21.979354573202098	31.921670839192018	24.752014815322884
50	20.563942522034363	22.148084639390923	29.92352275455262	27.364450084022085
51	19.4079358002675	22.306663465825302	27.678864158578826	30.606536575328374
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	222.0
1	535.5
2	849.0
3	3279.0
4	5709.0
5	3665.5
6	1622.0
7	1611.5
8	1601.0
9	1748.5
10	1896.0
11	1970.5
12	2045.0
13	2048.5
14	2052.0
15	1906.0
16	1760.0
17	1774.0
18	1788.0
19	1918.5
20	2049.0
21	1870.0
22	1691.0
23	2020.5
24	2350.0
25	3088.5
26	5145.0
27	6463.0
28	6914.5
29	7366.0
30	8896.5
31	10427.0
32	12802.5
33	15178.0
34	16958.0
35	18738.0
36	19315.0
37	19892.0
38	22852.0
39	25812.0
40	30393.5
41	34975.0
42	39885.5
43	44796.0
44	49371.0
45	53946.0
46	62331.0
47	70716.0
48	79945.5
49	89175.0
50	84704.0
51	80233.0
52	66697.0
53	53161.0
54	45360.0
55	37559.0
56	34144.5
57	30730.0
58	29217.0
59	27704.0
60	25792.5
61	23881.0
62	20517.5
63	17154.0
64	14248.5
65	11343.0
66	9499.0
67	7655.0
68	6305.0
69	4955.0
70	4076.5
71	3198.0
72	2744.0
73	2290.0
74	1680.5
75	834.5
76	598.0
77	469.0
78	340.0
79	208.0
80	76.0
81	67.0
82	58.0
83	34.5
84	11.0
85	8.5
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	728975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.38078265835827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.01338899134686	24.071151100865897
2	12.022239473889977	8.266680048370937
3	4.576847801078899	4.720668285278358
4	2.446444549978727	3.3644271343417493
5	1.5552634090671609	2.67355866218177
6	1.1309068986268038	2.332887857311569
7	0.8986901585428025	2.162836971264594
8	0.7104269169031824	1.9540026739756693
9	0.6116289428638227	1.8925453576946263
>10	5.849300024534788	39.52803838874391
>50	0.12804530337177583	2.9427449430161503
>100	0.050229990108855406	2.91528365156844
>500	0.0041172123040045415	1.0532957344656841
>1k	0.002470327382402725	2.1218791909206565
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	3436	0.4713467540039096	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	3219	0.4415789293185637	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2552	0.3500805926129154	No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2412	0.3308755444288213	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2369	0.3249768510579924	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1002	0.13745327343187352	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	959	0.13155458006104462	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	944	0.12949689632703454	No Hit
CTGTCACTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	895	0.12277512946260159	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	820	0.1124867107925512	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	750	0.10288418670050413	No Hit
GCTGTATCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	731	0.10027778730409137	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46517370280187936	0.0
2	0.0	0.0	0.0	1.6792071058678282	0.0
3	0.0	0.0	0.0	2.3678452621832022	0.0
4	0.0	0.0	0.0	3.064028258856614	0.0
5	0.0	0.0	0.0	4.205905552316609	0.0
6	0.0	0.0	0.0	4.9244487122329295	0.0
7	0.0	0.0	0.0	5.794163037141192	0.0
8	0.0	0.0	0.0	7.193250797352447	0.0
9	0.0	0.0	0.0	7.837031448266401	0.0
10	0.0	0.0	0.0	9.136390136835969	0.0
11	0.0	0.0	0.0	11.612058026681298	0.0
12	0.0	0.0	0.0	13.753832435954594	0.0
13	0.0	0.0	0.0	14.451935937446414	0.0
14	0.0	0.0	0.0	14.693645186734798	0.0
15	0.0	0.0	0.0	15.055660345004974	0.0
16	0.0	0.0	0.0	15.930038753043657	0.0
17	0.0	0.0	0.0	17.107033848897423	0.0
18	0.0	0.0	0.0	18.39747590795295	0.0
19	0.0	0.0	0.0	19.27747865153126	0.0
20	0.0	0.0	0.0	20.19424534449055	0.0
21	0.0	0.0	0.0	21.4694605439144	0.0
22	0.0	0.0	0.0	22.763743612606742	0.0
23	0.0	0.0	0.0	23.939778456051304	0.0
24	0.0	0.0	0.0	24.852292602626978	0.0
25	0.0	0.0	0.0	25.670702013100588	0.0
26	0.0	0.0	0.0	26.396241297712542	0.0
27	0.0	0.0	0.0	27.086388422099525	0.0
28	1.3717891560067216E-4	0.0	0.0	27.76501251757605	0.0
29	1.3717891560067216E-4	0.0	0.0	28.529236256387392	0.0
30	1.3717891560067216E-4	0.0	0.0	29.357522548784253	0.0
31	1.3717891560067216E-4	0.0	0.0	30.189512671902328	0.0
32	1.3717891560067216E-4	0.0	0.0	30.905860969169037	0.0
33	1.3717891560067216E-4	0.0	0.0	31.62220926643575	0.0
34	1.3717891560067216E-4	0.0	0.0	32.32237045166158	0.0
35	1.3717891560067216E-4	0.0	0.0	33.056140471209574	0.0
36	1.3717891560067216E-4	0.0	0.0	33.75959395040982	0.0
37	1.3717891560067216E-4	0.0	0.0	34.46318460852567	0.0
38	1.3717891560067216E-4	0.0	0.0	35.14564971363902	0.0
39	1.3717891560067216E-4	0.0	0.0	35.87118899825097	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	20	7.031586E-4	45.000004	26
ACGTTAT	20	7.031586E-4	45.000004	15
TCGATCC	20	7.031586E-4	45.000004	44
GCGTTAC	20	7.031586E-4	45.000004	25
AGTATAC	20	7.031586E-4	45.000004	14
CGTTACC	20	7.031586E-4	45.000004	19
AATTCGC	20	7.031586E-4	45.000004	13
ACTCGAT	20	7.031586E-4	45.000004	42
ACCACGC	20	7.031586E-4	45.000004	26
CGTAAGA	20	7.031586E-4	45.000004	39
CGACTCG	20	7.031586E-4	45.000004	13
CGTCAAC	20	7.031586E-4	45.000004	43
GCCCGTA	20	7.031586E-4	45.000004	30
TTTCGTA	25	3.889397E-5	45.0	23
GCGATAT	25	3.889397E-5	45.0	9
CGTAAAT	25	3.889397E-5	45.0	26
GCGTGTA	25	3.889397E-5	45.0	17
GGACCGT	35	1.2111013E-7	45.0	8
ACACGAC	30	2.1644046E-6	44.999996	26
ACGGACT	30	2.1644046E-6	44.999996	42
>>END_MODULE