SRR2934263.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934263.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467070
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	5595	1.1978932494058707	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	4660	0.9977091228295545	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	3115	0.6669235874708288	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	2739	0.5864217355000322	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1740	0.3725351660350697	No Hit
CCTGTATCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	1532	0.32800222664696943	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	1462	0.3130151797375126	No Hit
CTGTCACTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	1326	0.2838974885991393	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	1220	0.261202817564819	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	1216	0.2603464148842786	No Hit
CGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	1211	0.25927591153360313	No Hit
TCTGTATCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	999	0.2138865694649624	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	981	0.2100327574025307	No Hit
GCTGTATCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	786	0.16828312672618667	No Hit
TGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	750	0.16057550260132314	No Hit
TTCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	750	0.16057550260132314	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.13595392553578692	No Hit
GCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	624	0.13359881816430086	No Hit
TTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	554	0.11861177125484404	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	30	2.1627238E-6	45.000004	15
GACACGT	30	2.1627238E-6	45.000004	23
TAACGAA	30	2.1627238E-6	45.000004	19
ATTCCGA	30	2.1627238E-6	45.000004	12
ATATCCG	30	2.1627238E-6	45.000004	12
TGACACG	30	2.1627238E-6	45.000004	22
GGCGATA	60	0.0	45.000004	8
TTACACG	30	2.1627238E-6	45.000004	34
TATCCGT	30	2.1627238E-6	45.000004	13
AACAATC	25	3.887237E-5	45.0	35
ATCTCGC	20	7.028982E-4	45.0	11
TCGTTAG	50	2.1827873E-11	45.0	1
CGAACGA	25	3.887237E-5	45.0	22
ACACGCG	35	1.2098644E-7	45.0	36
GATCGGA	20	7.028982E-4	45.0	13
TACTTAG	20	7.028982E-4	45.0	15
GTTTCAC	20	7.028982E-4	45.0	37
CCCTATA	20	7.028982E-4	45.0	31
TTAGCCG	45	3.8380676E-10	45.0	1
CACGCGG	35	1.2098644E-7	45.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content