##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934263.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467070
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14905260453465	33.0	31.0	34.0	30.0	34.0
2	32.32133299077226	34.0	31.0	34.0	30.0	34.0
3	32.38566381912776	34.0	31.0	34.0	30.0	34.0
4	35.91795662320423	37.0	35.0	37.0	35.0	37.0
5	35.17156743100606	37.0	35.0	37.0	35.0	37.0
6	26.969589140814012	36.0	17.0	37.0	0.0	37.0
7	31.510675059412936	37.0	28.0	37.0	17.0	37.0
8	34.74237266362644	37.0	32.0	37.0	32.0	37.0
9	37.191500203395634	39.0	37.0	39.0	34.0	39.0
10	37.24356092234569	39.0	37.0	39.0	34.0	39.0
11	37.28287622840259	39.0	37.0	39.0	34.0	39.0
12	37.04996253238273	39.0	37.0	39.0	34.0	39.0
13	37.02808144389492	39.0	37.0	39.0	33.0	39.0
14	38.0841479863832	40.0	37.0	41.0	33.0	41.0
15	38.20104267026356	40.0	37.0	41.0	33.0	41.0
16	38.31056372706447	40.0	37.0	41.0	34.0	41.0
17	38.30930481512407	40.0	37.0	41.0	34.0	41.0
18	38.33828976384696	40.0	37.0	41.0	34.0	41.0
19	38.340246643971994	40.0	37.0	41.0	34.0	41.0
20	38.24565910891301	40.0	37.0	41.0	34.0	41.0
21	38.14085897188858	40.0	37.0	41.0	34.0	41.0
22	38.147513220716384	40.0	37.0	41.0	34.0	41.0
23	38.0830410859186	40.0	37.0	41.0	34.0	41.0
24	38.00991500203396	40.0	36.0	41.0	34.0	41.0
25	37.87206200355407	40.0	36.0	41.0	34.0	41.0
26	37.75961633159912	39.0	36.0	41.0	33.0	41.0
27	37.71890723017963	39.0	36.0	41.0	33.0	41.0
28	37.706772004196374	39.0	36.0	41.0	33.0	41.0
29	37.664566339092644	39.0	36.0	41.0	33.0	41.0
30	37.33937739525125	39.0	36.0	41.0	33.0	41.0
31	37.402749052604534	39.0	35.0	41.0	33.0	41.0
32	37.300008564026804	39.0	35.0	41.0	33.0	41.0
33	37.17621127454129	39.0	35.0	41.0	32.0	41.0
34	37.0900143447449	39.0	35.0	41.0	32.0	41.0
35	37.072687177510865	39.0	35.0	41.0	32.0	41.0
36	36.944995396835594	39.0	35.0	40.0	32.0	41.0
37	36.84324191234719	39.0	35.0	40.0	31.0	41.0
38	36.809660222236495	39.0	35.0	40.0	31.0	41.0
39	36.78495728691631	39.0	35.0	40.0	31.0	41.0
40	36.68480099342711	39.0	35.0	40.0	31.0	41.0
41	36.67312822489134	39.0	35.0	40.0	31.0	41.0
42	36.62057935641339	39.0	35.0	40.0	31.0	41.0
43	36.47002804718779	39.0	35.0	40.0	31.0	41.0
44	36.29658081229794	38.0	35.0	40.0	30.0	41.0
45	36.30502280172137	38.0	35.0	40.0	31.0	41.0
46	36.23737983599889	38.0	35.0	40.0	31.0	41.0
47	36.169753998330016	38.0	35.0	40.0	30.0	41.0
48	36.19601986854219	38.0	35.0	40.0	30.0	41.0
49	36.20725801271758	38.0	35.0	40.0	30.0	41.0
50	36.083869655512025	38.0	35.0	40.0	30.0	41.0
51	35.0280471877877	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	9.0
12	4.0
13	4.0
14	6.0
15	11.0
16	14.0
17	25.0
18	45.0
19	123.0
20	244.0
21	418.0
22	630.0
23	1022.0
24	1513.0
25	2282.0
26	3195.0
27	3722.0
28	4428.0
29	5326.0
30	7416.0
31	9836.0
32	13582.0
33	21452.0
34	31924.0
35	42492.0
36	49633.0
37	77851.0
38	103204.0
39	86624.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.89335645620571	4.7857922795298355	52.33862161988567	19.982229644378787
2	19.058813454086113	5.852655918813026	52.517181578778334	22.57134904832252
3	19.865544779155158	5.674310060590489	53.167833515318904	21.29231164493545
4	20.186695784357806	4.379643308283555	51.75348448840646	23.680176418952193
5	18.327659665574753	7.084162973430106	50.218810884878074	24.369366476117072
6	15.572826342946453	28.653306784850237	40.99171430406577	14.782152568137539
7	76.87027640396515	1.3734557989166505	14.6684651122958	7.087802684822403
8	76.29627250733296	2.730854047573169	11.912775386986961	9.060098058106922
9	70.96537992163915	4.472348898452052	15.734900550238724	8.827370629670071
10	37.91701458025564	25.823110026334383	20.8210760699681	15.438799323441883
11	26.1928618836577	23.520243218361273	28.97445779005288	21.312437107928147
12	22.806645684800994	20.377031280107907	34.8099856552551	22.006337379836
13	21.684329971952813	24.430171066435438	34.942085768728454	18.943413192883295
14	17.098721818999294	27.23767315391697	33.03658980452609	22.62701522255765
15	14.489262851392725	25.47112852463228	38.24694371293382	21.79266491104117
16	16.180658145459994	25.077611492923975	32.444173250262274	26.297557111353758
17	16.16652750123108	25.1698888817522	30.40657717258655	28.25700644443017
18	17.425867642965724	22.891857751514763	35.12385723767316	24.55841736784636
19	18.469180208534052	24.79692551437686	33.204872931252275	23.529021345836814
20	21.444109020061234	26.01794163615732	32.44310274691159	20.094846596869846
21	21.02554220994712	27.76007878904661	31.693322200098486	19.521056800907786
22	18.37219260496285	25.42745198792472	29.63281735071831	26.56753805639412
23	17.67657952769392	26.358361701672127	29.11704883636286	26.848009934271094
24	20.593701158284624	24.271094268525058	28.822874515597235	26.31233005759308
25	17.430149656368425	27.641895219132035	27.293553428822232	27.634401695677308
26	15.574539148307535	30.805446721048234	28.424219067805684	25.195795062838545
27	16.799409082150426	30.665210782109746	28.655661892221723	23.879718243518102
28	14.585394052283384	27.28884321407926	31.043740766908602	27.082021966728753
29	18.179073800500994	25.82674973772668	30.14751536172308	25.846661100049246
30	19.65015950499925	27.0347057186289	31.245423598175865	22.069711178195988
31	20.87609994219282	27.67936283640568	27.99623182820562	23.44830539319588
32	24.377074100241934	28.76892114672319	25.156400539533692	21.697604213501187
33	23.6290063587899	25.539640739075516	27.530991072002053	23.30036183013253
34	19.909007215192585	24.004110732866593	30.362258333868585	25.724623718072237
35	21.251204316269508	26.089023058642173	26.405249748431714	26.254522876656605
36	22.442888646241464	28.05082749909007	28.186781424625856	21.319502430042604
37	20.162288307962402	27.54918962896354	29.99721669128824	22.291305371785814
38	22.012974500610184	26.318110775686726	27.412379300747215	24.256535422955874
39	24.50767550902434	21.939537970753847	32.93553428822232	20.617252231999487
40	23.612306506519364	21.650073864731198	33.7058684993684	21.031751129381036
41	21.84383497120346	24.08075877277496	27.643822125163254	26.431584130858333
42	25.949215321043955	23.409767272571564	25.876635193868157	24.764382212516324
43	25.522298584794573	23.14171323356242	26.89618258505149	24.43980559659152
44	20.633095681589484	22.578200269766842	28.438563812704732	28.350140235938937
45	21.559081079923782	21.597833301218234	27.745948144817696	29.09713747404029
46	22.791444537221402	22.840045389342066	31.80700965594022	22.56150041749631
47	17.776778641317147	23.267818528271995	34.831181621598475	24.124221208812383
48	20.193118804461857	22.409274841030253	31.77660736078104	25.62099899372685
49	23.598818164300855	18.681353972637936	35.25617145181664	22.463656411244568
50	23.290513199306314	18.037125056201425	31.222086625131134	27.450275119361123
51	20.861112895283362	18.529770698182286	28.028132828055753	32.5809835784786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114.0
1	315.5
2	517.0
3	1996.5
4	3476.0
5	2167.0
6	858.0
7	968.0
8	1078.0
9	1287.5
10	1497.0
11	1723.5
12	1950.0
13	1940.5
14	1931.0
15	1888.0
16	1845.0
17	1808.5
18	1772.0
19	1810.5
20	1849.0
21	1822.0
22	1795.0
23	1839.0
24	1883.0
25	2105.5
26	2720.0
27	3112.0
28	3514.5
29	3917.0
30	4369.0
31	4821.0
32	5686.0
33	6551.0
34	7493.5
35	8436.0
36	9391.0
37	10346.0
38	12231.5
39	14117.0
40	17186.0
41	20255.0
42	23908.0
43	27561.0
44	32570.0
45	37579.0
46	48416.0
47	59253.0
48	68152.5
49	77052.0
50	70821.5
51	64591.0
52	50307.5
53	36024.0
54	28505.5
55	20987.0
56	17478.5
57	13970.0
58	12239.5
59	10509.0
60	9109.5
61	7710.0
62	6456.0
63	5202.0
64	4580.5
65	3959.0
66	3187.5
67	2416.0
68	2180.0
69	1944.0
70	1751.5
71	1559.0
72	1341.0
73	1123.0
74	831.5
75	437.5
76	335.0
77	235.0
78	135.0
79	112.0
80	89.0
81	64.0
82	39.0
83	28.5
84	18.0
85	20.0
86	22.0
87	12.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	467070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.46522211402424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.64707159383077	27.486371500418798
2	13.226197237004284	10.439496233645356
3	5.362132836465581	6.348532901880509
4	2.817013101242045	4.446961909545342
5	1.6918083731894211	3.338379661114326
6	1.2288253077856084	2.909751822665594
7	0.9093130279966438	2.5120368434744442
8	0.7118968698633366	2.2476134473148153
9	0.5677491357814426	2.0165711173793945
>10	3.658828149401889	23.923842960895325
>50	0.10816424659630478	2.926156957204415
>100	0.0604610404051129	4.422181244294577
>500	0.004437507552668837	1.3307479720838935
>1k	0.005546884440836047	4.4265594248245455
>5k	5.546884440836046E-4	1.2247960032586585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	5595	1.1978932494058707	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	4660	0.9977091228295545	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	3115	0.6669235874708288	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	2739	0.5864217355000322	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1740	0.3725351660350697	No Hit
CCTGTATCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	1532	0.32800222664696943	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	1462	0.3130151797375126	No Hit
CTGTCACTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	1326	0.2838974885991393	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	1220	0.261202817564819	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	1216	0.2603464148842786	No Hit
CGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	1211	0.25927591153360313	No Hit
TCTGTATCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	999	0.2138865694649624	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	981	0.2100327574025307	No Hit
GCTGTATCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	786	0.16828312672618667	No Hit
TGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	750	0.16057550260132314	No Hit
TTCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	750	0.16057550260132314	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.13595392553578692	No Hit
GCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	624	0.13359881816430086	No Hit
TTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	554	0.11861177125484404	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.049521485002248	0.0
2	0.0	0.0	0.0	3.7112210161217805	0.0
3	0.0	0.0	0.0	5.092812640503565	0.0
4	0.0	0.0	0.0	6.483396493031023	0.0
5	0.0	0.0	0.0	8.775130066157107	0.0
6	0.0	0.0	0.0	9.949900443188387	0.0
7	0.0	0.0	0.0	11.495707281563792	0.0
8	0.0	0.0	0.0	13.928104994968635	0.0
9	0.0	0.0	0.0	14.876999165007387	0.0
10	0.0	0.0	0.0	17.120988288693344	0.0
11	0.0	0.0	0.0	20.85019376110647	0.0
12	0.0	0.0	0.0	24.636778213115807	0.0
13	0.0	0.0	0.0	25.766159248078445	0.0
14	0.0	0.0	0.0	26.141691823495407	0.0
15	0.0	0.0	0.0	26.68443702228788	0.0
16	0.0	0.0	0.0	27.85257027854497	0.0
17	0.0	0.0	0.0	29.720384524803563	0.0
18	0.0	0.0	0.0	31.92776243389642	0.0
19	0.0	0.0	0.0	33.33782944740617	0.0
20	0.0	0.0	0.0	34.72734279658295	0.0
21	2.1410067013509753E-4	0.0	0.0	36.48125548632967	0.0
22	2.1410067013509753E-4	0.0	0.0	38.148885606011945	0.0
23	2.1410067013509753E-4	0.0	0.0	39.838568094718134	0.0
24	2.1410067013509753E-4	0.0	0.0	41.11353758537264	0.0
25	2.1410067013509753E-4	0.0	0.0	42.050870319224096	0.0
26	2.1410067013509753E-4	0.0	0.0	42.901278181000706	0.0
27	2.1410067013509753E-4	0.0	0.0	43.735842593187314	0.0
28	2.1410067013509753E-4	0.0	0.0	44.63527950842486	0.0
29	2.1410067013509753E-4	0.0	0.0	45.59894662470293	0.0
30	2.1410067013509753E-4	0.0	0.0	46.59515704284154	0.0
31	2.1410067013509753E-4	0.0	0.0	47.49673496478044	0.0
32	2.1410067013509753E-4	0.0	0.0	48.29554456505449	0.0
33	2.1410067013509753E-4	0.0	0.0	49.098422078061105	0.0
34	2.1410067013509753E-4	0.0	0.0	49.94476202710514	0.0
35	2.1410067013509753E-4	0.0	0.0	50.79452758687135	0.0
36	2.1410067013509753E-4	0.0	0.0	51.593765388485664	0.0
37	2.1410067013509753E-4	0.0	0.0	52.33005759308027	0.0
38	2.1410067013509753E-4	0.0	0.0	53.07148821375811	0.0
39	2.1410067013509753E-4	0.0	0.0	53.91247564604877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	30	2.1627238E-6	45.000004	15
GACACGT	30	2.1627238E-6	45.000004	23
TAACGAA	30	2.1627238E-6	45.000004	19
ATTCCGA	30	2.1627238E-6	45.000004	12
ATATCCG	30	2.1627238E-6	45.000004	12
TGACACG	30	2.1627238E-6	45.000004	22
GGCGATA	60	0.0	45.000004	8
TTACACG	30	2.1627238E-6	45.000004	34
TATCCGT	30	2.1627238E-6	45.000004	13
AACAATC	25	3.887237E-5	45.0	35
ATCTCGC	20	7.028982E-4	45.0	11
TCGTTAG	50	2.1827873E-11	45.0	1
CGAACGA	25	3.887237E-5	45.0	22
ACACGCG	35	1.2098644E-7	45.0	36
GATCGGA	20	7.028982E-4	45.0	13
TACTTAG	20	7.028982E-4	45.0	15
GTTTCAC	20	7.028982E-4	45.0	37
CCCTATA	20	7.028982E-4	45.0	31
TTAGCCG	45	3.8380676E-10	45.0	1
CACGCGG	35	1.2098644E-7	45.0	37
>>END_MODULE