SRR2934260.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934260.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562130
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2178	0.3874548591962713	RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	1730	0.3077579919235764	RNA PCR Primer, Index 30 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1307	0.23250849447636668	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1204	0.2141853307953676	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1198	0.21311796203725117	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	643	0.11438635191147956	RNA PCR Primer, Index 30 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	20	7.0302055E-4	45.000004	19
CGGGTAC	20	7.0302055E-4	45.000004	6
AAATCGG	20	7.0302055E-4	45.000004	40
ACACGGC	20	7.0302055E-4	45.000004	27
TCCGCGA	20	7.0302055E-4	45.000004	32
TTAATCG	20	7.0302055E-4	45.000004	20
CCGATAC	20	7.0302055E-4	45.000004	39
GGCGTTA	20	7.0302055E-4	45.000004	22
TTATGCG	30	2.163515E-6	45.000004	1
ATTACGA	20	7.0302055E-4	45.000004	20
CGACAAT	20	7.0302055E-4	45.000004	20
GTTACCG	20	7.0302055E-4	45.000004	1
CTACGTT	20	7.0302055E-4	45.000004	29
CGGATCC	20	7.0302055E-4	45.000004	25
TATTACG	20	7.0302055E-4	45.000004	19
ACTCGTT	20	7.0302055E-4	45.000004	31
AATTCGG	20	7.0302055E-4	45.000004	30
TCCGTGA	20	7.0302055E-4	45.000004	25
CCGAATA	20	7.0302055E-4	45.000004	6
CACGTTA	20	7.0302055E-4	45.000004	28