##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934260.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562130
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39040079696867	33.0	31.0	34.0	31.0	34.0
2	32.565129062672334	34.0	31.0	34.0	31.0	34.0
3	32.61697294220198	34.0	31.0	34.0	31.0	34.0
4	36.08121608880508	37.0	35.0	37.0	35.0	37.0
5	35.21797626883461	37.0	35.0	37.0	35.0	37.0
6	26.322092754345082	37.0	0.0	37.0	0.0	37.0
7	31.215042783697722	37.0	19.0	37.0	17.0	37.0
8	34.786359027271274	37.0	32.0	37.0	32.0	37.0
9	37.27519079216551	39.0	37.0	39.0	35.0	39.0
10	37.41159696155694	39.0	37.0	39.0	35.0	39.0
11	37.48473306886307	39.0	37.0	39.0	35.0	39.0
12	37.181166278263035	39.0	37.0	39.0	34.0	39.0
13	37.15290057460018	39.0	37.0	39.0	34.0	39.0
14	38.34949388931386	40.0	38.0	41.0	34.0	41.0
15	38.46071371390959	40.0	38.0	41.0	34.0	41.0
16	38.55488054364649	40.0	38.0	41.0	35.0	41.0
17	38.54383683489584	40.0	38.0	41.0	35.0	41.0
18	38.510406845391635	40.0	38.0	41.0	35.0	41.0
19	38.53766210663014	40.0	37.0	41.0	35.0	41.0
20	38.46782594773451	40.0	37.0	41.0	34.0	41.0
21	38.33874904381549	40.0	37.0	41.0	34.0	41.0
22	38.30581715973173	40.0	37.0	41.0	34.0	41.0
23	38.2621635564727	40.0	37.0	41.0	34.0	41.0
24	38.22420614448615	40.0	37.0	41.0	34.0	41.0
25	38.10090014765268	40.0	36.0	41.0	34.0	41.0
26	38.03649689573586	40.0	36.0	41.0	34.0	41.0
27	37.986640101044244	40.0	36.0	41.0	34.0	41.0
28	37.93063170441001	40.0	36.0	41.0	34.0	41.0
29	37.89618949353353	40.0	36.0	41.0	34.0	41.0
30	37.669800579937025	40.0	36.0	41.0	33.0	41.0
31	37.65003468948464	40.0	36.0	41.0	33.0	41.0
32	37.56914770604664	40.0	35.0	41.0	33.0	41.0
33	37.48359098429189	40.0	35.0	41.0	33.0	41.0
34	37.37616031878747	40.0	35.0	41.0	33.0	41.0
35	37.361521356269904	40.0	35.0	41.0	33.0	41.0
36	37.315923362923165	40.0	35.0	41.0	33.0	41.0
37	37.24272143454361	40.0	35.0	41.0	33.0	41.0
38	37.17194599114084	39.0	35.0	41.0	33.0	41.0
39	37.117213100172556	39.0	35.0	41.0	32.0	41.0
40	36.943374308433995	39.0	35.0	41.0	32.0	41.0
41	36.83306886307438	39.0	35.0	41.0	32.0	41.0
42	36.84098340241581	39.0	35.0	41.0	32.0	41.0
43	36.81745325814313	39.0	35.0	41.0	32.0	41.0
44	36.70239090601818	39.0	35.0	41.0	31.0	41.0
45	36.6337537580275	39.0	35.0	41.0	31.0	41.0
46	36.52189707007276	39.0	35.0	40.0	31.0	41.0
47	36.42198068062548	39.0	35.0	40.0	31.0	41.0
48	36.39891484176258	39.0	35.0	40.0	31.0	41.0
49	36.37792681408215	38.0	35.0	40.0	31.0	41.0
50	36.26171170369843	38.0	35.0	40.0	31.0	41.0
51	35.26297119883301	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	6.0
12	4.0
13	6.0
14	9.0
15	11.0
16	23.0
17	47.0
18	92.0
19	165.0
20	306.0
21	466.0
22	775.0
23	1138.0
24	1803.0
25	2654.0
26	3646.0
27	4428.0
28	5037.0
29	5936.0
30	7456.0
31	10071.0
32	14232.0
33	24070.0
34	36875.0
35	49939.0
36	51744.0
37	87588.0
38	123146.0
39	130364.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.395477914361447	5.022147901730916	52.27651966626937	19.30585451763827
2	20.025794744987817	4.96095209293224	53.906391759913184	21.106861402166757
3	21.094408766655402	5.152188995428104	51.382064646967784	22.371337590948713
4	19.760019924216817	4.850123636881149	50.2116948036931	25.17816163520894
5	17.631686620532616	8.629854304164516	48.651379574119865	25.087079501183002
6	16.352267269137034	31.15791720776333	39.03225232597442	13.45756319712522
7	82.78156298365147	2.5949513457741094	9.606852507427108	5.016633163147315
8	83.85462437514454	3.2609894508387742	8.035507800686673	4.848878373330013
9	78.68873748065394	5.3574795865725005	10.14996531051536	5.8038176222581965
10	40.54364648746731	26.202124063828652	17.691992955366196	15.562236493337839
11	31.484887837333	25.563303862095953	23.404372654012416	19.547435646558625
12	30.11118424563713	21.098144557308807	29.09149129204988	19.69917990500418
13	25.27333534947432	23.739704338854	30.13786846459004	20.849091847081635
14	20.278761140661413	27.473893939124398	29.445146140572465	22.80219877964172
15	17.725792966039883	25.577713340330526	36.2071051180332	20.489388575596394
16	21.03712664330315	22.882073541707435	34.57972355149165	21.50107626349777
17	20.924341344528845	23.31951683774216	29.850924163449736	25.905217654279262
18	21.076975076939497	23.807304360201375	31.496628893672284	23.619091669186844
19	24.872716275594613	24.914521551954174	28.444310035045273	21.768452137405937
20	28.239908917865975	24.519950901037127	28.185651005995055	19.054489175101843
21	26.521623112091508	24.412858235639444	28.754202764485083	20.311315887783966
22	22.80433351715795	24.552327753366658	28.059167808158254	24.584170921317135
23	22.94060092861082	25.891875544802804	27.52050237489549	23.64702115169089
24	23.6196253535659	22.862149324889263	29.0512870688275	24.466938252717345
25	23.522672691370325	24.56478038887802	27.28194545745646	24.6306014622952
26	21.586465764147082	27.960258303239467	27.105473822781207	23.347802109832244
27	21.030188746375394	27.364132851831425	29.27276608613666	22.332912315656518
28	19.677120950669774	26.280041983171152	30.362905377759592	23.67993168839948
29	22.121573301549464	25.174070054969487	29.601159874050488	23.10319676943056
30	23.094835714158645	24.964509988792628	28.243644708519383	23.697009588529344
31	23.36167790368776	28.095102556348177	25.213029014640743	23.330190525323324
32	25.014498425631082	28.026435166242685	25.045274224823437	21.913792183302792
33	24.386885595858608	26.418977816519313	25.749737605180297	23.444398982441783
34	21.56387312543362	27.41305391991176	26.851795847935534	24.171277106719085
35	21.282443562876914	26.965114831088894	26.764805294149042	24.98763631188515
36	22.461174461423514	27.456104459822463	26.22720722964439	23.855513849109634
37	21.49716257805134	27.794994040524436	27.522992901997757	23.184850479426466
38	22.742604023980217	27.033426431608348	25.460658566523758	24.763310977887677
39	24.23976660203156	25.173536370590433	26.515396794335828	24.071300233042177
40	24.20632238094391	25.689964954725774	27.989788838880685	22.11392382544963
41	19.184352374006014	27.455214985857364	27.17752121395407	26.182911426182553
42	21.270702506537635	26.439079928130504	25.85416184868269	26.436055716649175
43	21.652464732357284	23.70981801362674	28.261434187821322	26.37628306619465
44	22.731574546813015	24.296692935797772	27.437069716969386	25.534662800419834
45	22.9825840997634	24.258267660505577	25.942753455606354	26.816394784124668
46	22.19789016775479	26.447085193816378	27.15261594293135	24.202408695497482
47	20.180919004500737	26.59206945012719	29.315105046875278	23.911906498496787
48	21.18175511002793	23.58369060537598	29.31403767811716	25.920516606478927
49	22.11961645882625	22.81678615266931	30.185900058705283	24.877697329799155
50	21.760268976927044	24.25328660630103	27.69910874708697	26.287335669684946
51	19.939693665166423	25.667194421219293	25.25376692224219	29.139344991372102
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	467.5
2	747.0
3	2025.5
4	3304.0
5	2262.0
6	1220.0
7	1214.5
8	1209.0
9	1283.0
10	1357.0
11	1368.0
12	1379.0
13	1346.5
14	1314.0
15	1214.5
16	1115.0
17	1251.5
18	1388.0
19	1393.0
20	1398.0
21	1512.5
22	1627.0
23	2010.0
24	2393.0
25	2934.0
26	4116.0
27	4757.0
28	5437.0
29	6117.0
30	7119.0
31	8121.0
32	9335.5
33	10550.0
34	11702.0
35	12854.0
36	13988.0
37	15122.0
38	16969.0
39	18816.0
40	22397.5
41	25979.0
42	29484.0
43	32989.0
44	35993.0
45	38997.0
46	44168.0
47	49339.0
48	54565.0
49	59791.0
50	56807.0
51	53823.0
52	47666.0
53	41509.0
54	36720.5
55	31932.0
56	29264.5
57	26597.0
58	26683.0
59	26769.0
60	25722.5
61	24676.0
62	21007.5
63	17339.0
64	14434.0
65	11529.0
66	9772.5
67	8016.0
68	6669.5
69	5323.0
70	4322.0
71	3321.0
72	2877.5
73	2434.0
74	1950.0
75	1232.5
76	999.0
77	699.5
78	400.0
79	338.0
80	276.0
81	180.0
82	84.0
83	63.0
84	42.0
85	32.0
86	22.0
87	19.5
88	17.0
89	10.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.759457040736734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.62537032837565	23.136487852585443
2	11.152028242030248	7.3066878022374535
3	4.232172819102918	4.159310509691271
4	2.2592414091234896	2.9604608754733794
5	1.5004107032842582	2.457631998885112
6	1.082618892130342	2.127960425294036
7	0.9170909928204721	2.10303820872242
8	0.699736516086972	1.8338390670868756
9	0.6288080283031149	1.8539468631059635
>10	6.707084198935574	44.88661259417913
>50	0.14964956888073178	3.171768032936193
>100	0.04132114961632063	2.307072981797718
>500	0.001675181741202188	0.30182924357486796
>1k	0.0027919695686703127	1.393353544430163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2178	0.3874548591962713	RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	1730	0.3077579919235764	RNA PCR Primer, Index 30 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1307	0.23250849447636668	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1204	0.2141853307953676	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1198	0.21311796203725117	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	643	0.11438635191147956	RNA PCR Primer, Index 30 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7789479301940833E-4	0.0	0.0	0.32554747122551725	0.0
2	1.7789479301940833E-4	0.0	0.0	1.2184014373899277	0.0
3	1.7789479301940833E-4	0.0	0.0	1.7033426431608347	0.0
4	1.7789479301940833E-4	0.0	0.0	2.190952270827033	0.0
5	1.7789479301940833E-4	0.0	0.0	3.023144112571825	0.0
6	1.7789479301940833E-4	0.0	0.0	3.5434863821535942	0.0
7	1.7789479301940833E-4	0.0	0.0	4.193336061053493	0.0
8	1.7789479301940833E-4	0.0	0.0	5.189902691548219	0.0
9	1.7789479301940833E-4	0.0	0.0	5.626100724031808	0.0
10	1.7789479301940833E-4	0.0	0.0	6.571433654136944	0.0
11	1.7789479301940833E-4	0.0	0.0	8.255919449237721	0.0
12	1.7789479301940833E-4	0.0	0.0	9.895397861704588	0.0
13	1.7789479301940833E-4	0.0	0.0	10.420365395904861	0.0
14	1.7789479301940833E-4	0.0	0.0	10.596303346201056	0.0
15	1.7789479301940833E-4	0.0	0.0	10.885738174443635	0.0
16	1.7789479301940833E-4	0.0	0.0	11.577571024496113	0.0
17	1.7789479301940833E-4	0.0	0.0	12.551900805863413	0.0
18	1.7789479301940833E-4	0.0	0.0	13.60236955864302	0.0
19	1.7789479301940833E-4	0.0	0.0	14.309145571309127	0.0
20	1.7789479301940833E-4	0.0	0.0	15.088680554320176	0.0
21	1.7789479301940833E-4	0.0	0.0	16.081333499368473	0.0
22	3.5578958603881667E-4	0.0	0.0	17.1200611958088	0.0
23	3.5578958603881667E-4	0.0	0.0	18.11502677316635	0.0
24	3.5578958603881667E-4	0.0	0.0	18.873214381015067	0.0
25	3.5578958603881667E-4	0.0	0.0	19.561845124793198	0.0
26	3.5578958603881667E-4	0.0	0.0	20.143916887552702	0.0
27	3.5578958603881667E-4	0.0	0.0	20.740931812925837	0.0
28	3.5578958603881667E-4	0.0	0.0	21.312685677690215	0.0
29	3.5578958603881667E-4	0.0	0.0	21.937807980360414	0.0
30	3.5578958603881667E-4	0.0	0.0	22.611495561524915	0.0
31	3.5578958603881667E-4	0.0	0.0	23.29870314695889	0.0
32	3.5578958603881667E-4	0.0	0.0	23.9211570277338	0.0
33	3.5578958603881667E-4	0.0	0.0	24.570650917047658	0.0
34	3.5578958603881667E-4	0.0	0.0	25.14347215057015	0.0
35	3.5578958603881667E-4	0.0	0.0	25.76770497927526	0.0
36	3.5578958603881667E-4	0.0	0.0	26.42235781758668	0.0
37	3.5578958603881667E-4	0.0	0.0	27.07736644548414	0.0
38	3.5578958603881667E-4	0.0	0.0	27.681141372992013	0.0
39	3.5578958603881667E-4	0.0	0.0	28.350203689538006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	20	7.0302055E-4	45.000004	19
CGGGTAC	20	7.0302055E-4	45.000004	6
AAATCGG	20	7.0302055E-4	45.000004	40
ACACGGC	20	7.0302055E-4	45.000004	27
TCCGCGA	20	7.0302055E-4	45.000004	32
TTAATCG	20	7.0302055E-4	45.000004	20
CCGATAC	20	7.0302055E-4	45.000004	39
GGCGTTA	20	7.0302055E-4	45.000004	22
TTATGCG	30	2.163515E-6	45.000004	1
ATTACGA	20	7.0302055E-4	45.000004	20
CGACAAT	20	7.0302055E-4	45.000004	20
GTTACCG	20	7.0302055E-4	45.000004	1
CTACGTT	20	7.0302055E-4	45.000004	29
CGGATCC	20	7.0302055E-4	45.000004	25
TATTACG	20	7.0302055E-4	45.000004	19
ACTCGTT	20	7.0302055E-4	45.000004	31
AATTCGG	20	7.0302055E-4	45.000004	30
TCCGTGA	20	7.0302055E-4	45.000004	25
CCGAATA	20	7.0302055E-4	45.000004	6
CACGTTA	20	7.0302055E-4	45.000004	28
>>END_MODULE