FastQCFastQC Report
Sat 14 Jan 2017
SRR2934259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968870
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT29480.3042719869538741No Hit
CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC29330.30272379163355245No Hit
TCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC22540.23264215013366085No Hit
GCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC20280.2093160073074819No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.15647094037383757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT1000.045.0000044
TCGATAC253.8903498E-545.0000045
GGCGTAA207.032739E-445.022
TTATCCG207.032739E-445.01
GCCGCAA207.032739E-445.017
ATATCGG207.032739E-445.013
ATAGTCG351.2116652E-745.028
AATCGTG207.032739E-445.038
TAGGGCG2700.044.1666685
AGGGCGA4300.042.9069756
CGTTTAT4100.042.2560961
TGCGTAG750.042.0000041
TGTTCCG700.041.7857131
TTAGGGA19450.041.6452454
TTAGGGT10650.041.6197174
TAGGGCA9950.041.3819125
TGTAGGG14100.041.010643
TTTAGGG42450.040.9187283
GCGTTAG1100.040.9090921
TTTCGCG1650.040.909091
Produced by FastQC (version 0.11.5)