SRR2934258.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1548545
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	2423	0.15646946004152285	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2213	0.14290834299293853	No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2159	0.13942119860901686	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1858	0.119983597506046	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1726	0.11145946678979299	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1720	0.1110720063026906	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1675	0.10816605264942253	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	40	6.8175723E-9	45.0	22
ACGTTAG	40	6.8175723E-9	45.0	1
TCGAATG	20	7.0340466E-4	45.0	31
CGTTAGG	505	0.0	42.772278	2
TTAGGGA	4580	0.0	41.95415	4
TAGGGAT	3205	0.0	41.419655	5
GTTAGGG	2380	0.0	41.313026	3
CGTTTAT	610	0.0	41.311478	1
TATTGCG	60	3.6379788E-12	41.250004	1
TGTAGGG	2275	0.0	41.043953	3
CTAGGGA	2415	0.0	40.90062	4
TAGGGAC	2045	0.0	40.709045	5
TCTAGGG	2660	0.0	40.68609	3
TAGGGGT	1990	0.0	40.590454	5
CTTAGGG	3165	0.0	40.450237	3
CGTAGGG	610	0.0	40.204918	3
TCGTTAG	230	0.0	40.108692	1
TAGGGAA	1825	0.0	39.82192	5
TTTAGGG	6435	0.0	39.615383	3
AGGGATC	1285	0.0	39.571983	6