##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1548545
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42603347012841	33.0	31.0	34.0	31.0	34.0
2	32.59210420104033	34.0	31.0	34.0	31.0	34.0
3	32.653344914096785	34.0	31.0	34.0	31.0	34.0
4	36.09646668324137	37.0	35.0	37.0	35.0	37.0
5	35.161237161335315	37.0	35.0	37.0	35.0	37.0
6	25.882337290811698	37.0	0.0	37.0	0.0	37.0
7	31.022716162591337	37.0	19.0	37.0	17.0	37.0
8	34.80818058241769	37.0	32.0	37.0	32.0	37.0
9	37.34302716420898	39.0	37.0	39.0	35.0	39.0
10	37.41541446971189	39.0	37.0	39.0	35.0	39.0
11	37.430789547607596	39.0	37.0	39.0	35.0	39.0
12	37.16368332854389	39.0	37.0	39.0	34.0	39.0
13	37.16774843482107	39.0	37.0	39.0	34.0	39.0
14	38.10782637895573	40.0	38.0	41.0	33.0	41.0
15	38.312242782741215	40.0	38.0	41.0	33.0	41.0
16	38.4782424792305	40.0	38.0	41.0	34.0	41.0
17	38.49226984039857	40.0	38.0	41.0	34.0	41.0
18	38.47811397150228	40.0	38.0	41.0	34.0	41.0
19	38.48742077240248	40.0	38.0	41.0	34.0	41.0
20	38.39900035194328	40.0	37.0	41.0	34.0	41.0
21	38.321866009705886	40.0	37.0	41.0	34.0	41.0
22	38.36711235385475	40.0	37.0	41.0	34.0	41.0
23	38.3281441611319	40.0	37.0	41.0	34.0	41.0
24	38.29134639290431	40.0	37.0	41.0	34.0	41.0
25	38.18520869590486	40.0	37.0	41.0	34.0	41.0
26	38.143000687742365	40.0	37.0	41.0	34.0	41.0
27	38.13035591474578	40.0	37.0	41.0	34.0	41.0
28	38.06252966494355	40.0	36.0	41.0	34.0	41.0
29	38.03434514334423	40.0	36.0	41.0	34.0	41.0
30	37.81002747740621	40.0	36.0	41.0	34.0	41.0
31	37.81511289629943	40.0	36.0	41.0	33.0	41.0
32	37.7779586644237	40.0	36.0	41.0	33.0	41.0
33	37.75269817796706	40.0	36.0	41.0	33.0	41.0
34	37.64862499959639	40.0	36.0	41.0	33.0	41.0
35	37.625832636442595	40.0	36.0	41.0	33.0	41.0
36	37.602090995095395	40.0	36.0	41.0	33.0	41.0
37	37.53201230832814	40.0	36.0	41.0	33.0	41.0
38	37.46166239921991	40.0	35.0	41.0	33.0	41.0
39	37.41766755244439	40.0	35.0	41.0	33.0	41.0
40	37.345495287511824	40.0	35.0	41.0	33.0	41.0
41	37.253760788352935	40.0	35.0	41.0	33.0	41.0
42	37.24453212531764	40.0	35.0	41.0	32.0	41.0
43	37.20505635935669	40.0	35.0	41.0	32.0	41.0
44	37.107952303614034	39.0	35.0	41.0	32.0	41.0
45	37.082377974162846	39.0	35.0	41.0	32.0	41.0
46	37.0532932526985	39.0	35.0	41.0	32.0	41.0
47	36.982931719775664	39.0	35.0	41.0	32.0	41.0
48	36.92838503240138	39.0	35.0	41.0	32.0	41.0
49	36.91365443044923	39.0	35.0	41.0	32.0	41.0
50	36.82133357441986	39.0	35.0	41.0	32.0	41.0
51	35.884454116606236	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	16.0
11	14.0
12	4.0
13	10.0
14	11.0
15	30.0
16	83.0
17	148.0
18	238.0
19	473.0
20	770.0
21	1296.0
22	2118.0
23	3031.0
24	4610.0
25	6772.0
26	8883.0
27	10542.0
28	12692.0
29	15721.0
30	20331.0
31	28192.0
32	40882.0
33	67208.0
34	98406.0
35	109377.0
36	131485.0
37	230194.0
38	343980.0
39	410768.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.955713266324192	3.9853539935875286	56.08593873603931	15.972994004048962
2	20.171063805055713	4.261161283656594	54.53577390389043	21.032001007397266
3	21.522203100329666	4.3040402442292605	52.39660455459803	21.77715210084305
4	19.450516452540935	4.494670803883646	51.52959713795854	24.525215605616886
5	18.471274648137445	7.850918119912563	50.162119925478436	23.51568730647156
6	16.195654630637147	31.51164480205612	39.23024516562321	13.062455401683515
7	87.38816114481659	1.6774456021620296	7.456870804529412	3.4775224484919716
8	89.5150609120174	1.9608083717295912	5.343725884620724	3.180404831632274
9	84.23281209135027	4.622920225114543	7.092980830392401	4.051286853142789
10	41.37309538954309	33.3093323087156	13.52618102799725	11.791391273744063
11	28.378122689363238	23.196355288351324	29.466047160398958	18.95947486188648
12	27.743074951002395	21.271580741922254	30.986054651301703	19.999289655773644
13	26.498099829194505	21.754614815843258	32.46105214895273	19.286233206009513
14	20.69039001126864	24.450952345588924	33.897755635128455	20.960902008013974
15	18.781953382045728	25.409787897671688	35.756468168506565	20.051790551776023
16	25.40068257622478	25.025491671213945	29.970843598345542	19.60298215421573
17	25.366263169620513	24.16403785488959	29.035061945245378	21.43463703024452
18	24.86669744824981	23.13817163853811	31.450619775337497	20.544511137874586
19	25.128620737531037	25.165171176814365	29.118495103468096	20.587712982186503
20	24.352731112108465	29.51480260502601	28.3474487341343	17.785017548731226
21	24.723272491274066	25.968957957308312	31.285109570596916	18.022659980820706
22	22.843959975331682	22.732565085289743	32.668085202561116	21.755389736817467
23	21.53783067330946	24.599543442392697	32.11246686405626	21.750159020241583
24	21.912052927102536	26.019134090388075	29.958380286010417	22.11043269649897
25	23.103171041203193	26.591219499594782	27.32797561582001	22.977633843382016
26	21.015985973930366	26.752467638977233	27.60927192945636	24.62227445763604
27	20.36828119299084	25.125004439651416	32.14804865212183	22.358665715235915
28	18.810819188334857	26.431327471917186	29.81418040806047	24.943672931687487
29	20.561559399307093	26.660058312803308	29.37861024380951	23.399772044080088
30	23.384467354839543	24.216667904387666	30.041167676754633	22.357697064018158
31	23.357345120742373	25.427869387069794	29.530817638492906	21.68396785369492
32	24.045862406323355	26.140990413581783	27.59487131468572	22.218275865409144
33	23.983804151639116	24.71061544869539	28.147389969293755	23.158190430371736
34	20.779764230293598	24.335359966936704	30.85334943446913	24.031526368300565
35	19.79406475110507	26.521282881672796	29.290850443480814	24.393801923741318
36	22.51591009625164	25.646332525047704	28.049168735813296	23.78858864288736
37	21.11000971880055	27.1649839042456	29.251910664527024	22.473095712426826
38	22.5896567422968	26.48602397734648	28.66755567322874	22.25676360712798
39	20.35220158277609	24.949936876228975	28.558420969361563	26.139440571633372
40	22.21943824687045	23.513620850540345	29.542764336845234	24.72417656574397
41	19.894094133525343	23.711225698962572	30.019857349963996	26.37482281754809
42	19.819055952523176	24.2219631977114	32.060676312280236	23.89830453748519
43	20.144329031445647	23.9081847799063	31.7893893945607	24.158096794087353
44	21.459499078166925	24.166168887568652	29.242999073323666	25.131332960940757
45	20.843566057169795	23.395057941487007	27.92750614286314	27.83386985848006
46	20.969232408486675	24.305138048942716	29.189594102851384	25.536035439719218
47	18.430655873739543	24.764989070385425	32.254987746562094	24.549367309312935
48	19.180133609291303	26.307469269540118	30.696363360444806	23.816033760723776
49	21.987026531356854	23.79097798255782	30.559525231749802	23.66247025433552
50	20.408964544136595	24.085770836494902	28.87362007561937	26.631644543749132
51	18.844528250712766	23.965464355249605	28.383740866426226	28.806266527611402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	811.0
2	1374.0
3	3516.0
4	5658.0
5	3852.0
6	2046.0
7	2022.5
8	1999.0
9	2102.0
10	2205.0
11	2227.0
12	2249.0
13	2270.5
14	2292.0
15	2466.5
16	2641.0
17	2596.0
18	2551.0
19	2749.5
20	2948.0
21	3882.5
22	4817.0
23	6093.5
24	7370.0
25	9440.0
26	13520.0
27	15530.0
28	19422.0
29	23314.0
30	26772.5
31	30231.0
32	34441.0
33	38651.0
34	44492.5
35	50334.0
36	55207.0
37	60080.0
38	64451.0
39	68822.0
40	74812.5
41	80803.0
42	84965.0
43	89127.0
44	98703.5
45	108280.0
46	120485.5
47	132691.0
48	144413.5
49	156136.0
50	151768.5
51	147401.0
52	131338.5
53	115276.0
54	104467.5
55	93659.0
56	86551.5
57	79444.0
58	71769.5
59	64095.0
60	55984.0
61	47873.0
62	40614.0
63	33355.0
64	28055.0
65	22755.0
66	18832.0
67	14909.0
68	12197.5
69	9486.0
70	7833.0
71	6180.0
72	5552.5
73	4925.0
74	3887.0
75	2032.5
76	1216.0
77	932.0
78	648.0
79	454.5
80	261.0
81	238.5
82	216.0
83	141.0
84	66.0
85	39.0
86	12.0
87	9.0
88	6.0
89	4.5
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1548545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.091631456400826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.80269489782054	21.305686009852067
2	10.782324844194708	6.489154909094032
3	3.9844734852908856	3.596979230015227
4	2.0536213744254557	2.471872702007925
5	1.285877113619919	1.9347070100635526
6	1.00371384531295	1.812203227250652
7	0.8209275338894506	1.729213416154934
8	0.6925138757857059	1.6671097862869761
9	0.6113786479398299	1.6557642858706187
>10	7.737341003133019	49.90584420339257
>50	0.16921769002889261	3.2757381536798995
>100	0.05125604864433477	2.549761197352484
>500	0.003028765944310092	0.640297126702992
>1k	0.0016308739700131264	0.9656687422760333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	2423	0.15646946004152285	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2213	0.14290834299293853	No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2159	0.13942119860901686	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1858	0.119983597506046	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1726	0.11145946678979299	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1720	0.1110720063026906	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1675	0.10816605264942253	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9373024355120452E-4	0.0	0.0	0.16479986051422466	0.0
2	1.9373024355120452E-4	0.0	0.0	0.5556183385048545	0.0
3	1.9373024355120452E-4	0.0	0.0	0.7989435244051675	0.0
4	1.9373024355120452E-4	0.0	0.0	1.0976109832132743	0.0
5	1.9373024355120452E-4	0.0	0.0	1.6422512745835607	0.0
6	1.9373024355120452E-4	0.0	0.0	2.058577567975099	0.0
7	1.9373024355120452E-4	0.0	0.0	2.596243570577542	0.0
8	1.9373024355120452E-4	0.0	0.0	3.4717105411854354	0.0
9	1.9373024355120452E-4	0.0	0.0	3.9063120542186374	0.0
10	1.9373024355120452E-4	0.0	0.0	4.707063727563616	0.0
11	1.9373024355120452E-4	0.0	0.0	6.200594751847702	0.0
12	1.9373024355120452E-4	0.0	0.0	7.637039931032033	0.0
13	1.9373024355120452E-4	0.0	0.0	8.050266540526753	0.0
14	1.9373024355120452E-4	0.0	0.0	8.212612484622662	0.0
15	1.9373024355120452E-4	0.0	0.0	8.42300352911927	0.0
16	2.58306991401606E-4	0.0	0.0	8.867743591564984	0.0
17	2.58306991401606E-4	0.0	0.0	9.522358084524505	0.0
18	2.58306991401606E-4	0.0	0.0	10.219141193830337	0.0
19	2.58306991401606E-4	0.0	0.0	10.78418773752135	0.0
20	2.58306991401606E-4	0.0	0.0	11.399668721283527	0.0
21	2.58306991401606E-4	0.0	0.0	12.227671782221375	0.0
22	3.8746048710240903E-4	0.0	0.0	13.192319241610674	0.0
23	3.8746048710240903E-4	0.0	0.0	14.054225095169983	0.0
24	3.8746048710240903E-4	0.0	0.0	14.681846507528034	0.0
25	3.8746048710240903E-4	0.0	0.0	15.24101656716466	0.0
26	3.8746048710240903E-4	0.0	0.0	15.727021171486783	0.0
27	3.8746048710240903E-4	0.0	0.0	16.27340503504903	0.0
28	3.8746048710240903E-4	0.0	0.0	16.804548786118584	0.0
29	3.8746048710240903E-4	0.0	0.0	17.427456095883556	0.0
30	3.8746048710240903E-4	0.0	0.0	18.26630805046027	0.0
31	3.8746048710240903E-4	0.0	0.0	18.868809107904518	0.0
32	3.8746048710240903E-4	0.0	0.0	19.438440600692907	0.0
33	3.8746048710240903E-4	0.0	0.0	20.026282736375112	0.0
34	3.8746048710240903E-4	0.0	0.0	20.667658996025303	0.0
35	3.8746048710240903E-4	0.0	0.0	21.39763455372624	0.0
36	3.8746048710240903E-4	0.0	0.0	22.0182171005686	0.0
37	3.8746048710240903E-4	0.0	0.0	22.6379601496889	0.0
38	3.8746048710240903E-4	0.0	0.0	23.40345291870756	0.0
39	3.8746048710240903E-4	0.0	0.0	24.820977110771725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	40	6.8175723E-9	45.0	22
ACGTTAG	40	6.8175723E-9	45.0	1
TCGAATG	20	7.0340466E-4	45.0	31
CGTTAGG	505	0.0	42.772278	2
TTAGGGA	4580	0.0	41.95415	4
TAGGGAT	3205	0.0	41.419655	5
GTTAGGG	2380	0.0	41.313026	3
CGTTTAT	610	0.0	41.311478	1
TATTGCG	60	3.6379788E-12	41.250004	1
TGTAGGG	2275	0.0	41.043953	3
CTAGGGA	2415	0.0	40.90062	4
TAGGGAC	2045	0.0	40.709045	5
TCTAGGG	2660	0.0	40.68609	3
TAGGGGT	1990	0.0	40.590454	5
CTTAGGG	3165	0.0	40.450237	3
CGTAGGG	610	0.0	40.204918	3
TCGTTAG	230	0.0	40.108692	1
TAGGGAA	1825	0.0	39.82192	5
TTTAGGG	6435	0.0	39.615383	3
AGGGATC	1285	0.0	39.571983	6
>>END_MODULE