SRR2934257.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934257.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263527
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1783	0.6765910134445428	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	1597	0.6060100103594699	Illumina PCR Primer Index 7 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1209	0.45877652005297365	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1176	0.4462540840217511	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.4064099693769519	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	548	0.20794833166999965	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	525	0.19922057322399606	No Hit
CCTGTATCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	503	0.19087228253651428	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCACTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	476	0.18062665305642306	Illumina PCR Primer Index 7 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	466	0.17683197547120408	Illumina PCR Primer Index 7 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	426	0.1616532651303282	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	412	0.15634071651102166	Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	388	0.14723349030649613	Illumina PCR Primer Index 7 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	388	0.14723349030649613	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	383	0.14533615151388662	No Hit
GCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	335	0.12712169910483556	Illumina PCR Primer Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	276	0.10473310135204361	Illumina PCR Primer Index 7 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAT	20	7.02338E-4	45.000004	9
TTAGGGT	80	0.0	45.000004	4
GGCAGTC	20	7.02338E-4	45.000004	18
AACAACG	20	7.02338E-4	45.000004	38
GATACTA	20	7.02338E-4	45.000004	28
GGTACGA	20	7.02338E-4	45.000004	25
ACACGTC	20	7.02338E-4	45.000004	30
GATGTAC	40	6.7811925E-9	45.000004	9
CTCGTCA	20	7.02338E-4	45.000004	12
ATCTAGC	20	7.02338E-4	45.000004	12
GGTAAAT	20	7.02338E-4	45.000004	7
CGTGCTC	20	7.02338E-4	45.000004	21
ACGTTGC	20	7.02338E-4	45.000004	14
GCGGTAA	20	7.02338E-4	45.000004	32
CTATCAG	20	7.02338E-4	45.000004	38
TAGACGG	20	7.02338E-4	45.000004	2
TATGGTA	20	7.02338E-4	45.000004	10
TATGGAT	20	7.02338E-4	45.000004	12
ATGTGAG	20	7.02338E-4	45.000004	44
TTCACAT	20	7.02338E-4	45.000004	40

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content