##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934256.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957562
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2930149692657	33.0	31.0	34.0	31.0	34.0
2	32.46651078468026	34.0	31.0	34.0	31.0	34.0
3	32.477410340009314	34.0	31.0	34.0	30.0	34.0
4	35.9999968670436	37.0	35.0	37.0	35.0	37.0
5	35.09415473880542	37.0	35.0	37.0	35.0	37.0
6	25.73681704161193	36.0	0.0	37.0	0.0	37.0
7	30.949386044976723	37.0	19.0	37.0	17.0	37.0
8	34.766059012366824	37.0	32.0	37.0	32.0	37.0
9	37.37415749580706	39.0	37.0	39.0	35.0	39.0
10	37.2636925859631	39.0	37.0	39.0	34.0	39.0
11	37.312509268329364	39.0	37.0	39.0	34.0	39.0
12	37.222516139947075	39.0	37.0	39.0	34.0	39.0
13	37.30647832725192	39.0	37.0	39.0	34.0	39.0
14	38.56077308832222	40.0	38.0	41.0	34.0	41.0
15	38.67070017398351	40.0	38.0	41.0	35.0	41.0
16	38.699375079629306	40.0	38.0	41.0	35.0	41.0
17	38.69407202875636	40.0	38.0	41.0	35.0	41.0
18	38.65156825354389	40.0	38.0	41.0	35.0	41.0
19	38.60775699119222	40.0	38.0	41.0	35.0	41.0
20	38.52543438440539	40.0	38.0	41.0	35.0	41.0
21	38.420564934698746	40.0	38.0	41.0	34.0	41.0
22	38.40648960589497	40.0	37.0	41.0	34.0	41.0
23	38.332261514136945	40.0	37.0	41.0	34.0	41.0
24	38.28280048707029	40.0	37.0	41.0	34.0	41.0
25	38.157685873081846	40.0	37.0	41.0	34.0	41.0
26	38.1193301321481	40.0	37.0	41.0	34.0	41.0
27	38.09479699486822	40.0	37.0	41.0	34.0	41.0
28	38.03442388064689	40.0	37.0	41.0	34.0	41.0
29	37.99012178845861	40.0	37.0	41.0	34.0	41.0
30	37.74503374194047	40.0	36.0	41.0	33.0	41.0
31	37.732068524022466	40.0	36.0	41.0	33.0	41.0
32	37.57239426794296	40.0	36.0	41.0	33.0	41.0
33	37.41289023582807	40.0	36.0	41.0	33.0	41.0
34	37.29960879817704	40.0	36.0	41.0	32.0	41.0
35	37.2238298929991	40.0	36.0	41.0	32.0	41.0
36	37.1685864727297	40.0	36.0	41.0	32.0	41.0
37	37.098107485468304	40.0	36.0	41.0	32.0	41.0
38	36.97803693129009	40.0	36.0	41.0	31.0	41.0
39	36.91081517436991	40.0	36.0	41.0	31.0	41.0
40	36.9074033848461	40.0	36.0	41.0	31.0	41.0
41	36.85325649931806	40.0	36.0	41.0	31.0	41.0
42	36.80915387202082	40.0	36.0	41.0	31.0	41.0
43	36.74955459803125	40.0	35.0	41.0	31.0	41.0
44	36.61083251006201	39.0	35.0	41.0	31.0	41.0
45	36.56687922035336	39.0	35.0	41.0	30.0	41.0
46	36.483575998212125	39.0	35.0	41.0	30.0	41.0
47	36.419941476374376	39.0	35.0	41.0	30.0	41.0
48	36.360818411758196	39.0	35.0	41.0	30.0	41.0
49	36.325312616833166	39.0	35.0	41.0	30.0	41.0
50	36.235655759104894	39.0	35.0	41.0	30.0	41.0
51	35.293394056990564	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	0.0
12	1.0
13	6.0
14	6.0
15	8.0
16	29.0
17	59.0
18	120.0
19	294.0
20	537.0
21	960.0
22	1662.0
23	2644.0
24	4712.0
25	7639.0
26	9763.0
27	9902.0
28	9898.0
29	10460.0
30	12775.0
31	16561.0
32	22114.0
33	33357.0
34	51200.0
35	70927.0
36	89225.0
37	153001.0
38	218079.0
39	231491.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.304247662292365	4.2878685662129445	58.0426123843678	15.365271387126892
2	22.20848362821415	3.9716488331826034	53.08491773900802	20.734949799595224
3	18.947702603069043	3.8687834312556264	55.34095964543289	21.84255432024245
4	16.797345759334643	4.081615602958346	55.84265039757217	23.27838824013484
5	16.440293161173898	7.617783496003391	53.912018229629	22.029905113193713
6	13.940089519007644	31.533623932445103	42.590662536733916	11.935624011813333
7	83.32588385921747	1.1936563898734494	11.59663812891489	3.8838216219941892
8	85.67351252451539	1.3453959117007566	9.084215956773555	3.8968756070103034
9	81.85600514640305	3.0228852022114494	10.957828318166344	4.163281333219154
10	37.55370409435629	23.322667357309502	23.086233580697645	16.03739496763656
11	24.876613733627693	27.027701600522995	26.63911057456332	21.456574091285997
12	20.73442764019458	22.73106075637922	34.38095914415985	22.153552459266347
13	20.82162826010222	25.25068872824945	35.53148516754007	18.39619784410827
14	19.903045442488317	26.497500945108516	34.04688155962747	19.552572052775695
15	18.0632690102573	26.640572620885123	35.36972018522038	19.926438183637195
16	18.684116537623673	26.264304556780658	33.68523395874105	21.366344946854614
17	19.289299282970713	26.770172584125103	31.1095260672416	22.831002065662588
18	20.252683377159915	25.776085517178	33.35428933061254	20.61694177504955
19	20.810767344568813	29.748569805401637	30.326809125675414	19.11385372435414
20	21.823756581819246	27.9564143105094	31.149210181690584	19.070618925980774
21	21.10923365797724	28.60232548910671	31.62061568859249	18.667825164323563
22	20.48619306112816	25.500803081158192	30.474580236057825	23.53842362165583
23	18.841286517217686	27.807285585685314	29.64664429039582	23.704783606701184
24	19.716112377057566	26.796489417917584	31.564118041442747	21.9232801635821
25	18.425229906784104	28.84377199596475	29.566649470217072	23.164348627034073
26	18.81392536462391	28.782157186688696	30.187496997583445	22.21642045110395
27	20.471885893550496	27.960173858194036	30.22822543083372	21.339714817421743
28	19.055163007721692	26.458234558180045	31.708860627301416	22.777741806796843
29	19.30935020395546	25.569205962642627	31.763791796249226	23.357652037152686
30	21.394437122609293	25.983591662994144	31.054594898293793	21.567376316102767
31	20.888882390905238	28.570160470027005	29.43005257100846	21.1109045680593
32	19.029994924610627	29.976440167842917	28.38949331740399	22.604071590142468
33	20.406093809069283	28.226475152522763	28.51700464304139	22.850426395366565
34	18.60119762480132	27.58098170144596	29.37115299061575	24.446667683136965
35	17.964372019775222	27.501195745027474	28.644620400558917	25.889811834638383
36	21.484457403280413	27.128165069207004	28.04946311570426	23.33791441180832
37	20.152011044715643	28.865807122671956	29.752538216846535	21.229643615765873
38	19.381094905604023	30.804898272905568	28.57465104087255	21.23935578061786
39	18.43222684275274	28.4451555095127	31.084357984130534	22.03825966360403
40	19.40542753367406	26.721820623625415	31.671160718574882	22.201591124125645
41	18.14942531136365	27.327316664612837	29.31475977534614	25.20849824867737
42	19.9185013607474	27.23980274906481	27.016631821229332	25.825064068958458
43	20.728579454907358	26.256785461411376	28.51773566620229	24.496899417478975
44	20.169868896217686	26.71169073125291	27.75757601074395	25.360864361785453
45	20.79270062930651	25.899419567610245	27.91808781050209	25.389791992581156
46	21.189124046275854	26.640468189004995	28.636579145788993	23.53382861893016
47	19.00555786466046	27.07271174085856	29.992209381742384	23.9295210127386
48	19.63465551055702	26.616553288455474	29.631606099657255	24.117185101330254
49	20.84804952577483	24.710567044222724	30.546847097107026	23.894536332895417
50	19.922260908432037	24.775732537423163	29.73624684354642	25.565759710598375
51	18.508566547126975	24.55653002103258	28.860167801144993	28.074735630695454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	270.0
2	461.0
3	5426.0
4	10391.0
5	7007.0
6	3623.0
7	3635.5
8	3648.0
9	3845.5
10	4043.0
11	4292.0
12	4541.0
13	4608.0
14	4675.0
15	4562.0
16	4449.0
17	4518.0
18	4587.0
19	4383.0
20	4179.0
21	4152.0
22	4125.0
23	4461.0
24	4797.0
25	5359.0
26	7402.0
27	8883.0
28	10133.0
29	11383.0
30	13317.5
31	15252.0
32	18390.0
33	21528.0
34	24904.5
35	28281.0
36	30947.5
37	33614.0
38	37625.5
39	41637.0
40	47220.5
41	52804.0
42	59677.0
43	66550.0
44	74492.0
45	82434.0
46	94245.0
47	106056.0
48	113812.0
49	121568.0
50	113566.0
51	105564.0
52	88307.0
53	71050.0
54	58990.0
55	46930.0
56	39305.5
57	31681.0
58	26023.5
59	20366.0
60	17246.5
61	14127.0
62	11335.5
63	8544.0
64	6650.0
65	4756.0
66	3574.5
67	2393.0
68	1812.5
69	1232.0
70	884.0
71	536.0
72	467.5
73	399.0
74	321.0
75	180.0
76	117.0
77	88.0
78	59.0
79	45.0
80	31.0
81	25.5
82	20.0
83	12.0
84	4.0
85	2.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	957562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.940060272501555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47528416123535	32.72448474856362
2	11.12758941905421	9.778938995217851
3	4.147450982886197	5.467177384957959
4	2.115139882122979	3.717574956210222
5	1.4363236146133254	3.15560730984634
6	1.0497650140680659	2.7676042794068554
7	0.8162101784400889	2.510502710498073
8	0.6502906548549287	2.285904845517605
9	0.5474603889687523	2.1649898239284706
>10	3.4930627795012152	26.877298335351067
>50	0.0993394552474604	3.016012220486196
>100	0.037679385046464585	2.891447305889828
>500	0.0026913846428472067	0.8312575033346503
>1k	0.0017126993181754949	1.8111995807913677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4784	0.4996021145367089	No Hit
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	2800	0.29240926436095	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	2347	0.24510162266255348	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	2143	0.22379751911625564	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	1847	0.1928856825980981	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1731	0.18077158450314446	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	1195	0.1247960967540483	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3072385913392553	0.0
2	0.0	0.0	0.0	1.0194640138184263	0.0
3	0.0	0.0	0.0	1.5190661283551352	0.0
4	0.0	0.0	0.0	2.1070176134809024	0.0
5	0.0	0.0	0.0	3.0857531940490537	0.0
6	0.0	0.0	0.0	3.8497768290721646	0.0
7	0.0	0.0	0.0	4.738492128969194	0.0
8	0.0	0.0	0.0	6.127331702803578	0.0
9	0.0	0.0	0.0	6.712359095285736	0.0
10	0.0	0.0	0.0	7.916354241291947	0.0
11	0.0	0.0	0.0	10.311917139569031	0.0
12	0.0	0.0	0.0	12.2064158769876	0.0
13	0.0	0.0	0.0	12.884387642784487	0.0
14	0.0	0.0	0.0	13.113406755907189	0.0
15	0.0	0.0	0.0	13.384094189201326	0.0
16	0.0	0.0	0.0	14.14007656945451	0.0
17	0.0	0.0	0.0	15.323916362595842	0.0
18	0.0	0.0	0.0	16.571250738855554	0.0
19	0.0	0.0	0.0	17.473959910689857	0.0
20	0.0	0.0	0.0	18.299493923108894	0.0
21	0.0	0.0	0.0	19.58452820809514	0.0
22	0.0	0.0	0.0	21.026941336435655	0.0
23	0.0	0.0	0.0	22.586631466160938	0.0
24	0.0	0.0	0.0	23.69298280424662	0.0
25	0.0	0.0	0.0	24.641433139577384	0.0
26	0.0	0.0	0.0	25.44253009204626	0.0
27	0.0	0.0	0.0	26.25647216577099	0.0
28	0.0	0.0	0.0	27.133073367573065	0.0
29	0.0	0.0	0.0	28.039124359571495	0.0
30	0.0	0.0	0.0	28.960735701709133	0.0
31	0.0	0.0	0.0	29.866891125587692	0.0
32	0.0	0.0	0.0	30.70391264482091	0.0
33	0.0	0.0	0.0	31.594194422919873	0.0
34	0.0	0.0	0.0	32.37795568328735	0.0
35	1.0443188012891071E-4	0.0	0.0	33.234401532224545	0.0
36	1.0443188012891071E-4	0.0	0.0	34.03863144109729	0.0
37	1.0443188012891071E-4	0.0	0.0	34.872102276406125	0.0
38	1.0443188012891071E-4	0.0	0.0	35.69502549182194	0.0
39	1.0443188012891071E-4	0.0	0.0	36.48735016635999	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8903156E-5	45.000004	1
CGAACCT	25	3.8903156E-5	45.000004	13
CGCTAGG	130	0.0	45.000004	2
TACGAAC	30	2.1651213E-6	45.000004	11
TCGTCAG	30	2.1651213E-6	45.000004	1
GTCGTAG	30	2.1651213E-6	45.000004	1
CGTAAGA	25	3.8903156E-5	45.000004	18
AGGATCG	25	3.8903156E-5	45.000004	41
CGTCCGG	60	0.0	45.000004	2
TCGCGCA	25	3.8903156E-5	45.000004	34
TGCGTCG	25	3.8903156E-5	45.000004	1
TGTCACG	20	7.0326973E-4	45.0	1
CTATCGG	20	7.0326973E-4	45.0	2
TTCCGCG	40	6.8121153E-9	45.0	1
TCGTCCG	20	7.0326973E-4	45.0	1
CGAATAT	70	0.0	45.0	14
GCGATCC	20	7.0326973E-4	45.0	9
TAGGCGA	40	6.8121153E-9	45.0	5
CGTCAGG	75	0.0	45.0	2
CGTTTTT	3050	0.0	44.040985	1
>>END_MODULE