##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934255.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362235
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.378939086504616	33.0	31.0	34.0	31.0	34.0
2	32.5715322925725	34.0	31.0	34.0	31.0	34.0
3	32.567551451405855	34.0	31.0	34.0	31.0	34.0
4	36.057766367137354	37.0	35.0	37.0	35.0	37.0
5	35.256079616823335	37.0	35.0	37.0	35.0	37.0
6	26.571940867116652	37.0	8.0	37.0	0.0	37.0
7	31.34870180959874	37.0	19.0	37.0	17.0	37.0
8	34.867373390202495	37.0	32.0	37.0	32.0	37.0
9	37.3442544204729	39.0	37.0	39.0	35.0	39.0
10	37.46084724005135	39.0	37.0	39.0	35.0	39.0
11	37.46142421356302	39.0	37.0	39.0	35.0	39.0
12	37.08847019200243	39.0	37.0	39.0	34.0	39.0
13	37.029616133173214	39.0	37.0	39.0	33.0	39.0
14	37.966941350228446	40.0	37.0	41.0	33.0	41.0
15	38.17971758664955	40.0	37.0	41.0	33.0	41.0
16	38.356213507805705	40.0	37.0	41.0	34.0	41.0
17	38.397418802710945	40.0	37.0	41.0	34.0	41.0
18	38.351462448410565	40.0	37.0	41.0	34.0	41.0
19	38.30874156279763	40.0	37.0	41.0	34.0	41.0
20	38.15751652932489	40.0	36.0	41.0	34.0	41.0
21	38.04502049774318	40.0	36.0	41.0	34.0	41.0
22	38.06999875771253	40.0	36.0	41.0	34.0	41.0
23	38.01268789597913	40.0	35.0	41.0	34.0	41.0
24	37.94328543624995	40.0	35.0	41.0	34.0	41.0
25	37.80457161787238	40.0	35.0	41.0	34.0	41.0
26	37.76208814719726	40.0	35.0	41.0	34.0	41.0
27	37.74283269148481	40.0	35.0	41.0	34.0	41.0
28	37.6754316948942	39.0	35.0	41.0	34.0	41.0
29	37.62552762709291	39.0	35.0	41.0	34.0	41.0
30	37.32944635388629	39.0	35.0	41.0	33.0	41.0
31	37.26888345963256	39.0	35.0	41.0	33.0	41.0
32	37.098576890692506	39.0	35.0	41.0	33.0	41.0
33	36.89926428975665	39.0	35.0	41.0	32.0	41.0
34	36.73301585986997	39.0	35.0	41.0	31.0	41.0
35	36.62266484464505	39.0	35.0	41.0	31.0	41.0
36	36.50304084365122	39.0	35.0	41.0	31.0	41.0
37	36.43294270294146	39.0	35.0	41.0	31.0	41.0
38	36.353825555233485	39.0	35.0	41.0	31.0	41.0
39	36.2270901486604	39.0	35.0	41.0	30.0	41.0
40	36.11232763261419	39.0	35.0	41.0	30.0	41.0
41	35.976976272309415	39.0	35.0	41.0	30.0	41.0
42	35.92899913039877	39.0	35.0	40.0	30.0	41.0
43	35.861010117741245	38.0	35.0	40.0	30.0	41.0
44	35.75911217855812	38.0	35.0	40.0	29.0	41.0
45	35.74856929893578	38.0	35.0	40.0	29.0	41.0
46	35.69823181084103	38.0	35.0	40.0	29.0	41.0
47	35.60079230333899	38.0	35.0	40.0	29.0	41.0
48	35.54793987328668	38.0	35.0	40.0	29.0	41.0
49	35.51724433033804	38.0	35.0	40.0	28.0	41.0
50	35.40926194321366	38.0	35.0	40.0	28.0	41.0
51	34.36736096732784	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	5.0
11	2.0
12	5.0
13	4.0
14	5.0
15	10.0
16	19.0
17	45.0
18	77.0
19	141.0
20	231.0
21	432.0
22	726.0
23	1086.0
24	1935.0
25	3039.0
26	4093.0
27	4490.0
28	4637.0
29	4868.0
30	5778.0
31	7627.0
32	10657.0
33	17211.0
34	26370.0
35	31746.0
36	32760.0
37	53105.0
38	73507.0
39	77568.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.361270998108964	4.406531671428769	52.171380457437856	22.060816873024418
2	24.036882134525932	4.117768851712286	52.47477466285698	19.3705743509048
3	20.60430383590763	4.260769942164617	53.87773130702445	21.25719491490331
4	18.77924551741273	4.459535936615733	53.37529504327302	23.385923502698525
5	18.160862423564815	7.4067939321158915	51.20377655389458	23.228567090424722
6	15.959252971137522	29.580796996424972	41.396883238781456	13.063066793656052
7	81.28811406959571	1.8142918271287976	12.464560299253247	4.433033804022251
8	82.76836860049416	2.1585434869628832	10.63066793656052	4.4424199759824425
9	77.39478515328447	4.548980634118735	12.99570720664762	5.060527005949177
10	42.74352285118776	22.94863831490607	20.098278741700828	14.209560092205336
11	31.039518544591218	23.332643173630377	27.399340207323974	18.22849807445443
12	28.847019200242936	20.380968156031308	31.3699670103662	19.40204563335956
13	25.620384556986487	22.41003768271978	33.328364183472054	18.641213576821677
14	20.109321296948114	24.168840669730976	35.40795340041686	20.313884632904053
15	17.610667108368876	23.772965064115837	39.02577056330835	19.590597264206938
16	22.33494830703825	22.971551617044184	33.55860146037793	21.134898615539637
17	23.158170800723287	22.668709539387415	30.47331152428672	23.69980813560258
18	23.230223473711817	22.519635043549076	33.62568498350518	20.624456499233922
19	23.63603737904951	24.291413032975832	31.422419147790798	20.650130440183858
20	23.328502215412648	27.889905724184576	30.694714756994767	18.08687730340801
21	22.918547351857217	25.52900741231521	33.378883873728384	18.17356136209919
22	22.165445083992434	22.11740996866675	33.601391361961156	22.115753585379657
23	20.552679890126573	23.725758140433697	33.09509020387318	22.626471765566553
24	20.888097505762833	24.769003547420873	32.387262412522254	21.955636534294033
25	21.57466837826273	25.05031264234544	30.067497618949023	23.307521360442806
26	18.881389153450108	26.41572459867213	30.220713073005097	24.482173174872663
27	18.615815699752922	25.64108934807515	33.69304457051362	22.050050381658316
28	17.2570292765746	24.755752481124134	33.53182326390326	24.455394978398
29	19.59391003078112	24.766795036371416	32.452413488481234	23.186881444366225
30	22.672298369842782	24.02280287658564	32.52115339489558	20.783745358675997
31	23.33291923751156	24.98598975803001	30.579043990779464	21.102047013678966
32	24.52772371526771	25.61817604593703	28.727759603572267	21.12634063522299
33	24.434138059546978	23.64349110384143	30.084337515701133	21.83803332091046
34	21.24449597636893	23.742874101066988	31.504962248264253	23.507667674299835
35	21.533810923847778	26.15318784766795	29.661407649730148	22.651593578754124
36	23.661435256118267	24.98102060816873	30.24141786409375	21.116126271619255
37	21.62629232404378	25.947520256187286	30.374204590942345	22.051982828826592
38	23.779866661145387	24.350490703548804	29.0466133863376	22.82302924896821
39	22.536751004182367	23.768548042016924	30.71459135643988	22.98010959736083
40	25.324168012478086	22.627023893328914	30.388835976644994	21.659972117548
41	22.915234585283034	22.775270197523707	29.184369263047472	25.125125954145787
42	21.7309205350118	22.940080334589425	32.1042969343106	23.224702196088177
43	21.092660841718775	22.832415420928402	32.833105580631354	23.241818156721468
44	21.714356702140876	23.408284677074278	29.833671511587784	25.043687109197066
45	21.304125774704268	23.722997501621876	27.926622220381798	27.04625450329206
46	22.649385067704667	23.59517992463456	30.510028020483944	23.245406987176835
47	19.620964291136968	24.23095504299695	32.78507046530567	23.36301020056041
48	20.587740003036703	24.867558353002885	31.9314809446906	22.613220699269814
49	23.00661172995431	21.757422667605283	32.75277099120736	22.48319461123304
50	22.28001159468301	22.529849407152817	30.241969991856116	24.94816900630806
51	20.641296395986032	23.682416111088106	28.050298839151377	27.625988653774485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	236.5
2	403.0
3	3330.0
4	6257.0
5	4016.0
6	1775.0
7	1766.5
8	1758.0
9	1809.5
10	1861.0
11	1873.0
12	1885.0
13	1868.5
14	1852.0
15	1761.0
16	1670.0
17	1590.0
18	1510.0
19	1412.5
20	1315.0
21	1342.5
22	1370.0
23	1480.0
24	1590.0
25	1821.5
26	2661.0
27	3269.0
28	3792.5
29	4316.0
30	4744.5
31	5173.0
32	6170.0
33	7167.0
34	8120.5
35	9074.0
36	10081.0
37	11088.0
38	11739.5
39	12391.0
40	14258.0
41	16125.0
42	18435.5
43	20746.0
44	22538.5
45	24331.0
46	27960.0
47	31589.0
48	35192.0
49	38795.0
50	37029.5
51	35264.0
52	30023.5
53	24783.0
54	22589.5
55	20396.0
56	18607.0
57	16818.0
58	15617.5
59	14417.0
60	12959.5
61	11502.0
62	10058.0
63	8614.0
64	7582.0
65	6550.0
66	5765.5
67	4981.0
68	4208.5
69	3436.0
70	2979.5
71	2523.0
72	2031.0
73	1539.0
74	1269.5
75	753.0
76	506.0
77	388.0
78	270.0
79	189.5
80	109.0
81	92.5
82	76.0
83	44.0
84	12.0
85	8.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	362235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.507094999074425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53993276289232	30.93936070413991
2	9.277771924150267	7.701867212709013
3	3.657603568850018	4.554494964036341
4	2.2176228972075918	3.681883370660726
5	1.6220440623111687	3.366316849351713
6	1.2566993608183157	3.1297163852857155
7	1.0038895608668652	2.9167977560036054
8	0.806912737950561	2.6794082936061745
9	0.6524214667140424	2.4372107818501707
>10	4.8874300290964525	33.30682052932452
>50	0.05356664078786057	1.5413690329503658
>100	0.02008749029544771	1.6222450496450207
>500	0.001339166019696514	0.3507406634661669
>1k	0.002678332039393028	1.771768406970534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2999	0.8279155796651345	No Hit
CCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	1200	0.33127665741852663	No Hit
CTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCT	1100	0.3036702693003161	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.29704473615194554	No Hit
TCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	686	0.1893798224909244	No Hit
GCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	576	0.1590127955608928	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCC	430	0.11870746890830539	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	418	0.11539470233412011	No Hit
CGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	364	0.1004872527502864	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7606388118210553E-4	0.0	0.0	0.32575537979488456	0.0
2	2.7606388118210553E-4	0.0	0.0	1.0628459425511063	0.0
3	2.7606388118210553E-4	0.0	0.0	1.4852236807597279	0.0
4	2.7606388118210553E-4	0.0	0.0	2.0028434579761756	0.0
5	2.7606388118210553E-4	0.0	0.0	2.874377130868083	0.0
6	2.7606388118210553E-4	0.0	0.0	3.4149102102226454	0.0
7	2.7606388118210553E-4	0.0	0.0	4.129363534721935	0.0
8	2.7606388118210553E-4	0.0	0.0	5.24052065647991	0.0
9	2.7606388118210553E-4	0.0	0.0	5.730534045578147	0.0
10	2.7606388118210553E-4	0.0	0.0	6.685715074468232	0.0
11	2.7606388118210553E-4	0.0	0.0	8.680000552127762	0.0
12	2.7606388118210553E-4	0.0	0.0	10.524107278424227	0.0
13	2.7606388118210553E-4	0.0	0.0	11.067677060471793	0.0
14	2.7606388118210553E-4	0.0	0.0	11.261197841180449	0.0
15	2.7606388118210553E-4	0.0	0.0	11.520697889491629	0.0
16	2.7606388118210553E-4	0.0	0.0	12.15564481621047	0.0
17	2.7606388118210553E-4	0.0	0.0	13.010614656231452	0.0
18	2.7606388118210553E-4	0.0	0.0	13.895951523182465	0.0
19	2.7606388118210553E-4	0.0	0.0	14.552983560395875	0.0
20	2.7606388118210553E-4	0.0	0.0	15.269921459825804	0.0
21	2.7606388118210553E-4	0.0	0.0	16.25795409057656	0.0
22	2.7606388118210553E-4	0.0	0.0	17.32411279970185	0.0
23	2.7606388118210553E-4	0.0	0.0	18.291716703245132	0.0
24	2.7606388118210553E-4	0.0	0.0	19.027703010476625	0.0
25	2.7606388118210553E-4	0.0	0.0	19.685563239333582	0.0
26	2.7606388118210553E-4	0.0	0.0	20.222507488232775	0.0
27	2.7606388118210553E-4	0.0	0.0	20.787334189131364	0.0
28	2.7606388118210553E-4	0.0	0.0	21.29418747498171	0.0
29	2.7606388118210553E-4	0.0	0.0	21.912294504948445	0.0
30	2.7606388118210553E-4	0.0	0.0	22.664844645050866	0.0
31	2.7606388118210553E-4	0.0	0.0	23.270252736483222	0.0
32	2.7606388118210553E-4	0.0	0.0	23.83866826783718	0.0
33	2.7606388118210553E-4	0.0	0.0	24.39880188275567	0.0
34	2.7606388118210553E-4	0.0	0.0	25.0138722100294	0.0
35	2.7606388118210553E-4	0.0	0.0	25.676425524866453	0.0
36	2.7606388118210553E-4	0.0	0.0	26.278244785843444	0.0
37	2.7606388118210553E-4	0.0	0.0	26.877855535770976	0.0
38	2.7606388118210553E-4	0.0	0.0	27.534059381340843	0.0
39	2.7606388118210553E-4	0.0	0.0	28.840117603213383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGC	65	0.0	45.000004	4
TAGGGCC	75	0.0	45.000004	5
TCGTTTG	70	0.0	45.0	1
ATGATCG	20	7.0268824E-4	45.0	27
GGTAACA	40	6.7921064E-9	45.0	8
ATCCGGT	20	7.0268824E-4	45.0	21
CCATCGA	20	7.0268824E-4	45.0	15
TATTCGA	20	7.0268824E-4	45.0	14
GCGATTA	20	7.0268824E-4	45.0	23
ATGACCG	20	7.0268824E-4	45.0	42
TCCGTAG	35	1.2088458E-7	45.0	1
CTATTCG	20	7.0268824E-4	45.0	13
ATAGTCG	20	7.0268824E-4	45.0	14
ATTGCGT	20	7.0268824E-4	45.0	12
CCGTTAG	20	7.0268824E-4	45.0	1
TAGTCGA	20	7.0268824E-4	45.0	15
ATTTCGG	20	7.0268824E-4	45.0	2
TGTCCGA	20	7.0268824E-4	45.0	12
TTTCACG	30	2.1613705E-6	44.999996	1
GTAGGGA	130	0.0	43.269234	4
>>END_MODULE