FastQCFastQC Report
Sat 14 Jan 2017
SRR2934254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880382
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC74950.8513349886753705No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG60630.6886783237276546No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC47950.5446499360504872Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC24200.27488067679711764Illumina Single End Adapter 1 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT23520.2671567569532317Illumina Single End Adapter 1 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC20320.23080889886435663Illumina Single End Adapter 1 (95% over 24bp)
GAATCAGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC16120.1831023351227081Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC15860.180149071652987Illumina Single End Adapter 1 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC15430.17526482822229442No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT14990.1702669977350741No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.16924471422632448No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA12260.13925773130300256No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT12220.13880338307689163No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT10730.12187891165425918Illumina Single End Adapter 1 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC10250.11642673294092792Illumina Single End Adapter 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG1700.045.01
TATCTCG207.032387E-445.01
CGTACCG207.032387E-445.029
CGTAACG207.032387E-445.021
ATACGCC207.032387E-445.039
CGATAGG253.8900587E-545.02
CCGTTAG551.8189894E-1245.01
CGCTTAT253.8900587E-545.028
GGTCGAA302.1649212E-644.99999610
CGTTAGG2950.042.7118642
GTTAGGG12750.042.529413
TAGGGCA3850.042.0779235
ATCAGTC2100.041.7857133
TTTAGGG30000.041.4750023
TTAGGGG29300.041.3907854
TTAGGGA15350.041.1889274
TTCGTCG556.002665E-1140.9090921
CTTAGGG11550.040.7142873
TAGGGGA14150.040.388695
TAGGGAC4800.040.3124965
Produced by FastQC (version 0.11.5)