FastQCFastQC Report
Sat 14 Jan 2017
SRR2934253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320134
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC295182.2359851348423723No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG265912.014265218530846No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC191351.4494740685415268No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT65470.49593450361857205No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC62690.4748760353115669No Hit
GAATCAGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC57080.43238034926757435No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTAGGGTT53920.40844338529270513No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC49270.3732196883043691No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTA48130.36458420130077707No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT42440.3214825161688132No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32270.24444488211045243No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC25080.1899807140790253No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT24210.18339047399733663Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGT18730.1418795364712976No Hit
GCTGTATCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC15050.11400357842461448No Hit
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC14190.10748908822892221No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTAT13480.10211084632317628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG302.1657488E-644.9999962
TACGGCT31950.043.0281687
TTAGGGA19100.042.526184
CGTTTAT5550.042.1621631
ACGGCTG33100.041.6691868
TGATACC30850.041.6450544
GATACCT30850.041.572125
TCGTTAG2500.041.41
CGTTAGG4250.041.2941172
TTTAGGG41950.040.5482753
TCGAGGG6500.040.53
CTTAGGG21050.040.1900253
CGGCTGT35350.039.8444149
TAGGGAG12650.039.6640325
ATCAGTC7150.039.650353
GGCGACT6200.039.55645410
CGTTTTT17450.039.4555851
TAGGGAC8450.039.4082835
CCGATCG800.039.37540
GAATGAT114250.039.3282281
Produced by FastQC (version 0.11.5)