FastQCFastQC Report
Sat 14 Jan 2017
SRR2934250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509173
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC31300.6147223045998119No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC29000.5695510170413592TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT28700.5636591099685175Illumina PCR Primer Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG26640.5232013480683383No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC22100.4340371543660014TruSeq Adapter, Index 13 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC21780.4277524534883036TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC20310.39888210883137953TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18720.3676550013453188No Hit
CTGTCACTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT8580.1685085422832711Illumina PCR Primer Index 4 (95% over 23bp)
GAATCAGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC8130.15967068167400864TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC8030.15770671264972808Illumina PCR Primer Index 4 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC7620.14965443965017783RNA PCR Primer, Index 13 (96% over 25bp)
GCTGTATCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC7470.146708486113757RNA PCR Primer, Index 13 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG7450.1463156923089009Illumina PCR Primer Index 4 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC7360.1445481201870484No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT7190.14120937284577148No Hit
CGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG7070.13885261001663482Illumina PCR Primer Index 4 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.12373004852967459No Hit
TGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG5510.10821469323785825RNA PCR Primer, Index 13 (95% over 24bp)
GCTTGTGGGGAGAGGCTAAATATCAATTTGATTTTGTGTATCAAAGGAATA5300.10409035828686909No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA5290.10389396138444104No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG5130.10075161094559218TruSeq Adapter, Index 13 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG207.029581E-445.041
TAATACG207.029581E-445.04
ATCTCGG253.8877337E-545.041
CACGGTA207.029581E-445.021
ATGGCCG253.8877337E-545.011
CTATCGA207.029581E-445.018
GCGCGGA253.8877337E-545.030
CGTCTTA207.029581E-445.010
TATGCGC207.029581E-445.017
CGTTCCG207.029581E-445.013
GCCGCTA253.8877337E-545.09
CCGTCGT207.029581E-445.016
ATCCGTC207.029581E-445.014
ATCCGAT253.8877337E-545.010
CCGTATG207.029581E-445.01
TGCGCTA253.8877337E-545.019
AGGCCGC253.8877337E-545.036
AGTGATG253.8877337E-545.034
GTTCGCG207.029581E-445.01
AGCATCG207.029581E-445.017
Produced by FastQC (version 0.11.5)