SRR2934249.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934249.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1126980
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15116	1.3412837849828745	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	11532	1.0232657190012244	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG	10449	0.9271681839961669	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	8624	0.7652309712683455	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5485	0.4866989653764929	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	3713	0.3294645867717262	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3391	0.30089265115618735	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3321	0.2946813608049832	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	2805	0.2488952776446787	No Hit
GAATCAGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	2654	0.2354966370299384	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	2617	0.23221352641573054	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC	2515	0.22316278904683315	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	2426	0.21526557702887364	No Hit
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	2327	0.20648103781788496	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	2318	0.20568244334415875	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	2089	0.18536265062379104	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATAGTGT	2079	0.18447532343076187	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	1947	0.172762604482777	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA	1922	0.1705442865002041	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	1383	0.1227173507959325	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	1287	0.11419900974285258	No Hit
CTGTCACTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	1131	0.10035670553159771	TruSeq Adapter, Index 14 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCA	35	1.2119017E-7	45.000004	17
TCCGGCG	25	3.890758E-5	45.0	7
GATCGTA	20	7.033228E-4	45.0	9
GTCGAAT	20	7.033228E-4	45.0	43
CGCGGCC	20	7.033228E-4	45.0	35
TCTAGCG	25	3.890758E-5	45.0	1
TCTCGAG	50	2.1827873E-11	45.0	1
TCCGTAG	40	6.8157533E-9	45.0	1
GTGCGTC	20	7.033228E-4	45.0	37
TCGCGCA	20	7.033228E-4	45.0	34
TCGCGAG	20	7.033228E-4	45.0	1
TACGTTG	20	7.033228E-4	45.0	1
TATCCGG	20	7.033228E-4	45.0	2
ATCCCCG	30	2.165465E-6	44.999996	38
CGTTTAT	2730	0.0	44.752747	1
CGTTTTT	6750	0.0	44.3	1
TTCGTAG	215	0.0	42.906975	1
CGTTAGG	305	0.0	42.786884	2
TCGCTAG	100	0.0	42.75	1
CCTCGTC	80	0.0	42.1875	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content