SRR2934248.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1488169
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	4007	0.2692570534663738	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	3586	0.24096725573506772	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	3322	0.2232273350674554	RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	3198	0.21489494808721324	TruSeq Adapter, Index 3 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	2825	0.18983059047729123	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	2409	0.16187677609196266	RNA PCR Primer, Index 24 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.13983626859583823	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1982	0.1331837983454836	RNA PCR Primer, Index 24 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1807	0.12142438123627089	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	25	3.8913615E-5	45.000004	28
CGACCGT	20	7.033959E-4	45.0	42
CCGTCGC	20	7.033959E-4	45.0	14
AACGCGC	20	7.033959E-4	45.0	40
CGATCGT	20	7.033959E-4	45.0	10
CGATATT	20	7.033959E-4	45.0	11
TAGGGCG	685	0.0	43.357666	5
TTTCGCG	345	0.0	41.73913	1
CGTCATA	65	0.0	41.538464	38
TTAGGGA	2705	0.0	41.090572	4
TCGTTAG	220	0.0	40.909092	1
TTTAGGG	5695	0.0	40.77261	3
CGTTTAT	510	0.0	40.147057	1
TTAGGGG	5365	0.0	39.79963	4
CTTAGGG	2575	0.0	39.757282	3
CACGACC	1145	0.0	39.69432	27
TGTAGGG	1990	0.0	39.685932	3
TCTCGAG	125	0.0	39.6	1
AACACGT	1075	0.0	39.55814	41
GCGATCT	405	0.0	39.444447	9