##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934247.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1483523
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42108885403192	33.0	31.0	34.0	31.0	34.0
2	32.58356762921775	34.0	31.0	34.0	31.0	34.0
3	32.64600076978921	34.0	31.0	34.0	31.0	34.0
4	36.09701029239183	37.0	35.0	37.0	35.0	37.0
5	35.163713673465125	37.0	35.0	37.0	35.0	37.0
6	25.776440270895698	37.0	0.0	37.0	0.0	37.0
7	30.927090446187893	37.0	19.0	37.0	17.0	37.0
8	34.649933300663356	37.0	32.0	37.0	32.0	37.0
9	37.25422659439725	39.0	37.0	39.0	35.0	39.0
10	37.4013796887544	39.0	37.0	39.0	35.0	39.0
11	37.45632861775652	39.0	37.0	39.0	35.0	39.0
12	37.17739866520438	39.0	37.0	39.0	34.0	39.0
13	37.15920481178924	39.0	37.0	39.0	34.0	39.0
14	38.190603044239964	40.0	38.0	41.0	33.0	41.0
15	38.33728968138681	40.0	38.0	41.0	33.0	41.0
16	38.52337779731086	40.0	38.0	41.0	34.0	41.0
17	38.486470381652325	40.0	38.0	41.0	34.0	41.0
18	38.48832003278682	40.0	38.0	41.0	34.0	41.0
19	38.49686051379049	40.0	38.0	41.0	34.0	41.0
20	38.37283008082787	40.0	37.0	41.0	34.0	41.0
21	38.33878746740024	40.0	37.0	41.0	34.0	41.0
22	38.3789297503308	40.0	37.0	41.0	34.0	41.0
23	38.328166802941375	40.0	37.0	41.0	34.0	41.0
24	38.28720956803501	40.0	37.0	41.0	34.0	41.0
25	38.1933006768348	40.0	37.0	41.0	34.0	41.0
26	38.12602433531533	40.0	37.0	41.0	34.0	41.0
27	38.086384235364065	40.0	37.0	41.0	34.0	41.0
28	38.01723936871892	40.0	36.0	41.0	34.0	41.0
29	37.981101068200495	40.0	36.0	41.0	34.0	41.0
30	37.77301194521419	40.0	36.0	41.0	33.0	41.0
31	37.75427142012628	40.0	36.0	41.0	33.0	41.0
32	37.675342411273704	40.0	36.0	41.0	33.0	41.0
33	37.6274348291196	40.0	36.0	41.0	33.0	41.0
34	37.49302167880107	40.0	36.0	41.0	33.0	41.0
35	37.47894100731839	40.0	36.0	41.0	33.0	41.0
36	37.40443457903922	40.0	36.0	41.0	33.0	41.0
37	37.32615335252638	40.0	35.0	41.0	32.0	41.0
38	37.204569123633405	40.0	35.0	41.0	32.0	41.0
39	37.196388596604166	40.0	35.0	41.0	32.0	41.0
40	37.10734245441426	40.0	35.0	41.0	32.0	41.0
41	37.005454583447644	40.0	35.0	41.0	32.0	41.0
42	36.99327411843294	39.0	35.0	41.0	31.0	41.0
43	36.95199130717892	39.0	35.0	41.0	32.0	41.0
44	36.85566856732252	39.0	35.0	41.0	31.0	41.0
45	36.83376799685613	39.0	35.0	41.0	31.0	41.0
46	36.83224864056708	39.0	35.0	41.0	31.0	41.0
47	36.698825700713776	39.0	35.0	41.0	31.0	41.0
48	36.676462043392654	39.0	35.0	41.0	31.0	41.0
49	36.67620050380075	39.0	35.0	41.0	31.0	41.0
50	36.579588587436795	39.0	35.0	41.0	31.0	41.0
51	35.63009201744766	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	16.0
11	12.0
12	9.0
13	17.0
14	12.0
15	29.0
16	75.0
17	134.0
18	309.0
19	566.0
20	1128.0
21	1956.0
22	2838.0
23	3943.0
24	5340.0
25	7371.0
26	9253.0
27	11065.0
28	12819.0
29	15414.0
30	20115.0
31	27907.0
32	39344.0
33	64527.0
34	92667.0
35	109175.0
36	129406.0
37	221542.0
38	326238.0
39	379975.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.069938922416437	4.081163554592682	52.43828373405738	15.410613788933505
2	19.426190224216274	8.103817736563572	51.92275414671697	20.547237892503183
3	20.648213745253695	8.511630760021921	49.75521107525802	21.084944419466364
4	18.203560039177013	4.190834924702886	53.79822220484617	23.807382831273934
5	20.853805434765757	7.608914725285689	47.95732860225288	23.579951237695674
6	15.803395026568513	33.66540323271025	37.912523095361514	12.61867864535973
7	83.75421210186832	1.831586028662852	10.736200247653727	3.678001621815098
8	85.31044008080765	4.69665788801387	6.585809589740098	3.407092441438387
9	80.51294115426589	4.437207916560782	7.609453982176212	7.440396946997114
10	41.357970183138384	28.82307857714373	15.934771486522283	13.884179753195603
11	30.410853084178672	24.005020481650774	27.35252503668632	18.231601397484233
12	27.018455393007052	22.093894061635712	30.234246452532247	20.653404092824985
13	25.820159175152664	22.343300373502803	32.9990165302459	18.83752392109863
14	20.935233225234796	25.963601508031893	32.445401925012284	20.655763341721027
15	18.82956988196341	24.442560041199226	36.65113382131588	20.076736255521485
16	24.299724372321833	25.20736112618409	29.127152056287635	21.365762445206443
17	24.50484421205468	24.683877499708466	29.940149225863028	20.87112906237382
18	24.12837549535801	23.38163951620568	31.109190757406523	21.380794231029785
19	24.102828200169462	25.7788386159163	28.87956573642606	21.238767447488176
20	24.18809819598348	28.638720127696033	29.78174251427177	17.39143916204872
21	24.42537122781379	27.472509694827785	30.396225741023226	17.705893336335198
22	22.74187862271094	22.184017369464442	32.66063283144245	22.413471176382167
23	21.35976321229937	26.35968569412136	31.80644991685333	20.47410117672594
24	21.65049008340282	26.759679492667114	29.524112534824198	22.065717889105866
25	22.410707484818236	29.154586750593015	26.574377343661006	21.86032842092775
26	21.034928342870316	26.556312237828468	28.391740471836297	24.01701894746492
27	21.356797299401492	26.666792493274453	30.03357548214621	21.942834725177836
28	17.95745667576438	28.24155742782552	30.43437816602776	23.36660773038234
29	21.72099792183876	27.84068733683266	28.362890228193294	22.075424513135285
30	24.366390005412793	26.07192473591579	29.013031816830615	20.54865344184081
31	23.56626759409864	27.40779886796497	27.322191836594378	21.703741701342008
32	24.98538950862238	28.865140614604563	26.028312334894704	20.121157541878354
33	25.329233183442383	25.2222580977848	26.02231310198763	23.42619561678518
34	21.463233128168554	27.535602751019027	28.89075531690442	22.110408803907994
35	21.610248037947507	29.679216297961002	26.712224886301055	21.998310777790437
36	24.075797948531974	29.97230241796049	25.45481263182303	20.497087001684502
37	22.27151179995187	29.5695449278508	26.98347110223434	21.175472169962987
38	22.713028379067936	29.454548395946677	26.11776157161028	21.71466165337511
39	22.58286524711784	26.659445118141072	26.893347794405614	23.86434184033547
40	24.171785675045147	26.04550114828014	27.5298057394459	22.252907437228814
41	21.77964210868318	26.719572261434436	26.103606078234044	25.397179551648343
42	21.56097343957593	26.880473036144366	29.27187512428186	22.286678399997843
43	23.37294399884599	25.506109443534076	28.117528343005134	23.003418214614804
44	22.85128036437588	25.568730649946108	27.926901032205098	23.653087953472916
45	21.171764778840636	24.893715837233398	27.017174657892056	26.91734472603391
46	23.108101458487667	26.291065254802255	27.378881217210655	23.221952069499427
47	19.718804494436554	26.612934211333428	31.713023660570144	21.955237633659877
48	21.046252737571308	28.90450636761277	27.7049968217547	22.34424407306122
49	22.920979317476036	25.88668999402099	29.550064272680636	21.642266415822338
50	21.885201645003143	25.712981868161126	27.5651944728865	24.836622013949228
51	20.839245498721624	25.78753413327599	26.168856161987375	27.204364206015008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	172.0
1	819.5
2	1467.0
3	4300.5
4	7134.0
5	4813.5
6	2493.0
7	2456.0
8	2419.0
9	2429.0
10	2439.0
11	2466.5
12	2494.0
13	2561.5
14	2629.0
15	2651.5
16	2674.0
17	2768.0
18	2862.0
19	3246.5
20	3631.0
21	4232.5
22	4834.0
23	5955.0
24	7076.0
25	8303.0
26	12446.5
27	15363.0
28	18534.0
29	21705.0
30	24020.5
31	26336.0
32	31911.5
33	37487.0
34	41439.5
35	45392.0
36	50770.0
37	56148.0
38	60363.5
39	64579.0
40	71702.5
41	78826.0
42	84055.5
43	89285.0
44	96349.0
45	103413.0
46	134146.5
47	164880.0
48	155482.5
49	146085.0
50	139457.5
51	132830.0
52	120008.0
53	107186.0
54	98435.5
55	89685.0
56	80413.0
57	71141.0
58	63894.5
59	56648.0
60	49160.5
61	41673.0
62	35262.5
63	28852.0
64	24092.5
65	19333.0
66	15739.0
67	12145.0
68	10413.5
69	8682.0
70	7098.5
71	5515.0
72	4613.0
73	3711.0
74	3069.0
75	1888.0
76	1349.0
77	991.0
78	633.0
79	415.5
80	198.0
81	132.5
82	67.0
83	62.5
84	58.0
85	44.0
86	30.0
87	17.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1483523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.569654718717054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69225561366322	21.19915244502026
2	9.827264225336863	5.811776199456229
3	3.57976397760317	3.1755715437668073
4	1.9651089129709154	2.3243036816489346
5	1.285851605954218	1.901109400378702
6	1.0841316433583712	1.9234439018245402
7	0.9361979856402456	1.937813582861834
8	0.76215494776716	1.8029326918109403
9	0.6979232708891986	1.8573615126316108
>10	7.983782666040852	48.46742784477954
>50	0.13803106903618667	2.6515219629708007
>100	0.04050097822616755	2.023083299705525
>500	0.002910249729718738	0.5531190244698034
>1k	0.003395291351338527	2.0461029438833696
>5k	2.425208108098948E-4	0.5996604800488132
>10k+	4.850416216197896E-4	1.7256194847422377
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	12518	0.8438022194465471	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	11545	0.7782151001366342	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	8362	0.5636582648196219	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	3030	0.20424354728575156	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2765	0.18638066278716273	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCT	2749	0.18530214900611583	No Hit
GAATCAGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	2681	0.18071846543666664	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC	2619	0.17653922453511	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	2232	0.15045267245603877	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT	2126	0.14330751865660324	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTA	2020	0.1361623648571677	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1961	0.13218534528955736	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCT	1608	0.10839063499521072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16042892493072233	0.0
2	0.0	0.0	0.0	0.6258750285637634	0.0
3	0.0	0.0	0.0	0.8567443848191096	0.0
4	0.0	0.0	0.0	1.218046501469812	0.0
5	0.0	0.0	0.0	2.2385901667854156	0.0
6	0.0	0.0	0.0	2.6164744328197136	0.0
7	0.0	0.0	0.0	3.240933912045853	0.0
8	0.0	0.0	0.0	4.1021945733230964	0.0
9	0.0	0.0	0.0	4.447386390369411	0.0
10	0.0	0.0	0.0	5.928320625969398	0.0
11	0.0	0.0	0.0	7.117449476684891	0.0
12	0.0	0.0	0.0	9.069154977711838	0.0
13	0.0	0.0	0.0	9.472653946046	0.0
14	0.0	0.0	0.0	9.652563526146881	0.0
15	0.0	0.0	0.0	9.986296134269573	0.0
16	0.0	0.0	0.0	10.449922245897097	0.0
17	0.0	0.0	0.0	11.04762110193101	0.0
18	0.0	0.0	0.0	11.65071252687016	0.0
19	0.0	0.0	0.0	12.29182156259121	0.0
20	0.0	0.0	0.0	12.812204461946326	0.0
21	0.0	0.0	0.0	13.518968024088606	0.0
22	0.0	0.0	0.0	14.342211074583947	0.0
23	0.0	0.0	0.0	15.081667085714209	0.0
24	0.0	0.0	0.0	15.637169090064663	0.0
25	0.0	0.0	0.0	16.136858006245944	0.0
26	0.0	0.0	0.0	16.577228664469644	0.0
27	0.0	0.0	0.0	17.098757484717122	0.0
28	0.0	0.0	0.0	17.568180607917775	0.0
29	6.740711131542956E-5	0.0	0.0	18.133928493188176	0.0
30	6.740711131542956E-5	0.0	0.0	18.82896321796157	0.0
31	6.740711131542956E-5	0.0	0.0	19.351233516433517	0.0
32	6.740711131542956E-5	0.0	0.0	19.84276617214563	0.0
33	6.740711131542956E-5	0.0	0.0	20.352700969246854	0.0
34	6.740711131542956E-5	0.0	0.0	20.906382981591793	0.0
35	6.740711131542956E-5	0.0	0.0	21.501924810063613	0.0
36	6.740711131542956E-5	0.0	0.0	22.022779559197936	0.0
37	6.740711131542956E-5	0.0	0.0	22.54855502745829	0.0
38	6.740711131542956E-5	0.0	0.0	23.184878158275943	0.0
39	6.740711131542956E-5	0.0	0.0	24.325945738623535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGAC	20	7.0339505E-4	45.0	10
TCGTTAG	180	0.0	43.750004	1
CGTTTAT	610	0.0	42.418034	1
TTAGGGA	3730	0.0	41.621983	4
CGTAAGG	65	0.0	41.53846	2
TAGGGAC	1680	0.0	41.11607	5
ACACGAC	540	0.0	40.833332	26
CGATTAG	50	1.0822987E-9	40.5	10
ATAGTCG	50	1.0822987E-9	40.5	16
CACGACC	545	0.0	40.458714	27
TCTAGGG	2635	0.0	40.388996	3
CTAGGGA	2010	0.0	40.298508	4
TTTAGGG	5910	0.0	40.126904	3
CGTTAGG	490	0.0	39.94898	2
GTTAGGG	2220	0.0	39.93243	3
TTTCGCG	305	0.0	39.836067	1
CTTAGGG	2590	0.0	39.700775	3
TACGGCT	1545	0.0	39.611652	7
TATTGCG	40	3.4591358E-7	39.375	1
CGACCAA	555	0.0	39.324326	29
>>END_MODULE