##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289190
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.348584770282116	33.0	31.0	34.0	31.0	34.0
2	32.518279695002285	34.0	31.0	34.0	31.0	34.0
3	32.57944368169161	34.0	31.0	34.0	31.0	34.0
4	36.04706210876597	37.0	35.0	37.0	35.0	37.0
5	35.1632342788883	37.0	35.0	37.0	35.0	37.0
6	26.218008206703434	37.0	0.0	37.0	0.0	37.0
7	31.1626641534607	37.0	19.0	37.0	17.0	37.0
8	34.750748144183554	37.0	32.0	37.0	32.0	37.0
9	37.250927326460804	39.0	37.0	39.0	35.0	39.0
10	37.39038776285885	39.0	37.0	39.0	35.0	39.0
11	37.43259488516045	39.0	37.0	39.0	35.0	39.0
12	37.08506581652045	39.0	37.0	39.0	34.0	39.0
13	37.059149543511815	39.0	37.0	39.0	33.0	39.0
14	37.99341679659321	40.0	37.0	41.0	33.0	41.0
15	38.18827403253206	40.0	37.0	41.0	33.0	41.0
16	38.33762284845523	40.0	37.0	41.0	34.0	41.0
17	38.35635786811874	40.0	37.0	41.0	34.0	41.0
18	38.34182626300235	40.0	37.0	41.0	34.0	41.0
19	38.365924340089514	40.0	37.0	41.0	34.0	41.0
20	38.27952745522382	40.0	37.0	41.0	34.0	41.0
21	38.18394030360149	40.0	37.0	41.0	34.0	41.0
22	38.228435684422	40.0	37.0	41.0	34.0	41.0
23	38.20080360536461	40.0	36.0	41.0	34.0	41.0
24	38.144833577672806	40.0	36.0	41.0	34.0	41.0
25	38.01658793506	40.0	36.0	41.0	34.0	41.0
26	37.98452982105043	40.0	36.0	41.0	34.0	41.0
27	37.94564261280339	40.0	36.0	41.0	34.0	41.0
28	37.860969290795	40.0	36.0	41.0	34.0	41.0
29	37.8237342827667	40.0	36.0	41.0	34.0	41.0
30	37.57286668373165	40.0	36.0	41.0	33.0	41.0
31	37.546287203592954	40.0	35.0	41.0	33.0	41.0
32	37.49709585088311	40.0	35.0	41.0	33.0	41.0
33	37.40193997781553	40.0	35.0	41.0	33.0	41.0
34	37.2861983105671	40.0	35.0	41.0	32.0	41.0
35	37.242161357131224	40.0	35.0	41.0	32.0	41.0
36	37.160859919794596	39.0	35.0	41.0	32.0	41.0
37	37.077523095897426	39.0	35.0	41.0	32.0	41.0
38	36.967440796158826	39.0	35.0	41.0	32.0	41.0
39	36.82797182727139	39.0	35.0	41.0	31.0	41.0
40	36.657144408504564	39.0	35.0	41.0	31.0	41.0
41	36.49899006352826	39.0	35.0	41.0	31.0	41.0
42	36.434105135782936	39.0	35.0	41.0	31.0	41.0
43	36.331659414050684	38.0	35.0	40.0	31.0	41.0
44	36.20413748167454	38.0	35.0	40.0	30.0	41.0
45	36.1094415873533	38.0	35.0	40.0	30.0	41.0
46	35.98310722236443	37.0	35.0	40.0	30.0	41.0
47	35.83168656288057	37.0	34.0	40.0	30.0	41.0
48	35.72957981368146	37.0	34.0	40.0	30.0	41.0
49	35.657128894887485	37.0	34.0	40.0	30.0	41.0
50	35.49419868289391	36.0	34.0	40.0	30.0	41.0
51	34.496674656179465	35.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	15.0
10	20.0
11	18.0
12	19.0
13	18.0
14	19.0
15	38.0
16	65.0
17	158.0
18	214.0
19	450.0
20	713.0
21	1279.0
22	1988.0
23	2966.0
24	4383.0
25	6392.0
26	8334.0
27	10181.0
28	12322.0
29	15395.0
30	19893.0
31	27092.0
32	38600.0
33	63325.0
34	95656.0
35	112454.0
36	133328.0
37	207461.0
38	258614.0
39	267614.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.00990544450391	4.903311381565169	50.844406177522316	17.2423769964086
2	21.429502245596073	5.019818645816365	50.825789837029454	22.72488927155811
3	22.999790566169455	5.7090110844794015	49.29630232936961	21.99489601998154
4	20.368991382185715	4.8542883516006174	50.07446536197146	24.7022549042422
5	18.539237815992987	8.445768273101715	48.18917304664169	24.825820864263605
6	16.822888790635982	31.408791566797756	38.83182463407256	12.936495008493706
7	84.09582761268703	2.472870562135915	9.028071890101536	4.403229935075513
8	85.19000302515532	3.243044081942925	7.219727115475609	4.347225777426136
9	79.65606310939427	4.9110681901038635	9.48556845771376	5.947300242788107
10	43.636314274854755	24.019345480495506	17.088869755427826	15.255470489221915
11	32.78965862285621	23.76779217958563	23.97629519310575	19.466254004452406
12	29.788471831149792	21.49132400964947	28.57592752038102	20.144276638819726
13	27.81009781335567	21.302368153646864	30.67709181734267	20.21044221565479
14	21.822694870422513	23.863278492696967	32.831002412367454	21.483024224513066
15	20.007446536197147	23.585972587438626	35.479952528331744	20.926628348032487
16	25.184805963434403	23.780358209418317	29.9013333953878	21.133502431759478
17	25.572180981856825	22.855203655008182	28.10555465059456	23.467060712540434
18	25.365229330044446	22.416866404486537	30.698655745080245	21.51924852038877
19	25.56093360947572	24.144462802224652	28.515346845693806	21.77925674260582
20	25.652231245976154	27.746802255679924	27.8664122433466	18.734554254997324
21	25.31969686392231	24.975139428633483	30.45935820166151	19.2458055057827
22	23.689913821857136	22.597444907267352	30.996749897222287	22.715891373653225
23	22.675478401166625	23.893297341741714	30.841536158363002	22.589688098728658
24	23.23102102870795	24.661376523243277	28.813208293579688	23.294394154469085
25	23.7602680753031	25.37221045772927	26.450174140351695	24.41734732661594
26	22.090615037349032	25.19403656559545	27.013938984944037	25.70140941211148
27	22.25637803582094	24.256083277096472	30.46083199528386	23.026706691798726
28	20.365345682172528	24.881980158083756	29.269774044167267	25.482900115576445
29	23.452710616743847	25.341958904428363	27.832437421947116	23.372893056880677
30	25.741744816512696	23.980483869716643	27.8622235667357	22.41554774703496
31	24.045796197612454	25.877256261683694	27.3000100838511	22.776937456852753
32	27.1966118260303	26.225381828900318	24.722732878784353	21.855273466285034
33	26.126482520031956	25.656264786416276	25.48072820918561	22.736524484366154
34	22.56424576672174	25.62973650121394	28.55389818413112	23.252119547933198
35	23.486607870057945	27.745018189716024	25.666271069431197	23.10210287079484
36	26.08630225180152	27.56847322737533	24.32473103266392	22.020493488159232
37	23.67098720902272	27.98749602463562	25.6388895352896	22.702627231052055
38	23.61793063861805	27.425204973665636	25.40936557062962	23.547498817086698
39	22.75389973549283	26.611050349444227	26.04131276227709	24.59373715278586
40	23.732886541161506	26.70265825828621	27.124395938535052	22.440059262017236
41	21.311521187722523	26.90402500795073	25.912006763937047	25.8724470403897
42	21.705722197658993	26.468325072332238	27.61571219137598	24.210240538632785
43	22.02561298179477	24.887409924060844	27.910470915846386	25.176506178298002
44	23.655783864286878	23.835819390469982	26.966079476260283	25.54231726898285
45	23.364825976000432	23.38476097394488	25.596847633009872	27.653565417044813
46	23.410125737866412	24.834586057912333	26.968018678394962	24.787269525826293
47	20.827263630651803	25.7654806506411	29.409163893607616	23.998091825099483
48	20.849292966901697	26.932337359116964	28.21965730419876	23.998712369782577
49	23.68192430906228	24.309527687928078	28.90217888751852	23.10636911549112
50	22.05508885424181	25.340097270379076	26.621134200544528	25.983679674834587
51	20.684150513112883	25.688067701424927	25.47948711981942	28.14829466564277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	888.0
2	1508.0
3	3598.0
4	5688.0
5	3846.0
6	2004.0
7	1999.5
8	1995.0
9	2052.5
10	2110.0
11	2150.0
12	2190.0
13	2224.0
14	2258.0
15	2197.0
16	2136.0
17	2099.0
18	2062.0
19	2365.5
20	2669.0
21	3158.0
22	3647.0
23	4068.0
24	4489.0
25	5772.0
26	8430.5
27	9806.0
28	12187.5
29	14569.0
30	16899.0
31	19229.0
32	22487.0
33	25745.0
34	28961.0
35	32177.0
36	33742.0
37	35307.0
38	38303.5
39	41300.0
40	46883.5
41	52467.0
42	57906.5
43	63346.0
44	69910.5
45	76475.0
46	91121.5
47	105768.0
48	118585.5
49	131403.0
50	130264.5
51	129126.0
52	116719.5
53	104313.0
54	96567.5
55	88822.0
56	83367.5
57	77913.0
58	71595.0
59	65277.0
60	58980.5
61	52684.0
62	45687.5
63	38691.0
64	33889.5
65	29088.0
66	24609.5
67	20131.0
68	17044.5
69	13958.0
70	11497.5
71	9037.0
72	7429.5
73	5822.0
74	4514.0
75	2560.5
76	1915.0
77	1353.0
78	791.0
79	591.0
80	391.0
81	284.5
82	178.0
83	120.5
84	63.0
85	69.0
86	75.0
87	46.0
88	17.0
89	16.5
90	16.0
91	9.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1289190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.43447834753076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94401063820015	21.98653487945964
2	11.055940085760831	6.950754184748924
3	4.275405751274187	4.031854485460106
4	2.2506803386315157	2.8299584948770233
5	1.4662770974973045	2.3045827836379633
6	1.1737189513319537	2.2137145777038483
7	0.9465113622982801	2.0827163646699987
8	0.8287292817824617	2.0840538131324404
9	0.7201474551381937	2.0373713627013834
>10	7.143618338656486	45.61606705061376
>50	0.13739878085153642	2.822851151632151
>100	0.05193364219265946	2.7896568079790516
>500	0.0030699689369342305	0.6887892494399596
>1k	0.002558307447445192	1.5610947939437823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	3063	0.23759104554022292	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2687	0.20842544543473035	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2269	0.17600198574298592	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2215	0.1718133091320907	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	1830	0.14194959625811557	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	1823	0.14140661966040693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	1495	0.11596428765348786	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1492	0.11573158339732702	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1310	0.10161419185690239	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25434575198380377	0.0
2	0.0	0.0	0.0	0.8370372094105601	0.0
3	0.0	0.0	0.0	1.179965714906259	0.0
4	0.0	0.0	0.0	1.5746321333550524	0.0
5	0.0	0.0	0.0	2.336971276537981	0.0
6	0.0	0.0	0.0	2.782367222829839	0.0
7	0.0	0.0	0.0	3.3801068888216634	0.0
8	0.0	0.0	0.0	4.303322241097123	0.0
9	0.0	0.0	0.0	4.72102638090584	0.0
10	0.0	0.0	0.0	5.65897966940482	0.0
11	0.0	0.0	0.0	7.3368549244099	0.0
12	0.0	0.0	0.0	8.933749098271008	0.0
13	0.0	0.0	0.0	9.423048580891878	0.0
14	0.0	0.0	0.0	9.616580953932313	0.0
15	0.0	0.0	0.0	9.920880552905313	0.0
16	0.0	0.0	0.0	10.561903210543054	0.0
17	0.0	0.0	0.0	11.38024651137536	0.0
18	0.0	0.0	0.0	12.239080352779652	0.0
19	0.0	0.0	0.0	12.900193144532613	0.0
20	0.0	0.0	0.0	13.599857274722888	0.0
21	0.0	0.0	0.0	14.606535886874704	0.0
22	0.0	0.0	0.0	15.632373816117097	0.0
23	0.0	0.0	0.0	16.538136349180494	0.0
24	0.0	0.0	0.0	17.265337149683134	0.0
25	0.0	0.0	0.0	17.941420581915775	0.0
26	0.0	0.0	0.0	18.534816435125933	0.0
27	0.0	0.0	0.0	19.164203879955632	0.0
28	7.75680853869484E-5	0.0	0.0	19.76714060766838	0.0
29	7.75680853869484E-5	0.0	0.0	20.45579006973371	0.0
30	7.75680853869484E-5	0.0	0.0	21.3288964388492	0.0
31	7.75680853869484E-5	0.0	0.0	22.018166445597622	0.0
32	7.75680853869484E-5	0.0	0.0	22.674159743715045	0.0
33	7.75680853869484E-5	0.0	0.0	23.32790356735625	0.0
34	7.75680853869484E-5	0.0	0.0	24.025163086899525	0.0
35	7.75680853869484E-5	0.0	0.0	24.792311451376445	0.0
36	7.75680853869484E-5	0.0	0.0	25.44923556651851	0.0
37	7.75680853869484E-5	0.0	0.0	26.129973083874372	0.0
38	7.75680853869484E-5	0.0	0.0	26.89665603983897	0.0
39	7.75680853869484E-5	0.0	0.0	28.239825006399368	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAC	25	3.8910715E-5	45.0	15
ACGGATA	25	3.8910715E-5	45.0	24
TCGTTAG	230	0.0	42.065216	1
TTAGGGA	2345	0.0	41.449894	4
CTTAGGG	2285	0.0	40.9628	3
CGTTTAT	500	0.0	40.949997	1
TTTAGGG	4330	0.0	40.635105	3
GTTAGGG	1825	0.0	40.56164	3
TTCAGGG	1825	0.0	40.315067	3
TGTAGGG	1795	0.0	40.236767	3
TTAGGGG	4430	0.0	40.073364	4
TAGGGAT	1315	0.0	39.86692	5
ACACGCG	345	0.0	39.782608	36
CGTTAGG	415	0.0	39.578316	2
TTTTAGG	2440	0.0	39.559425	2
TAGGGAG	1350	0.0	39.5	5
TAGGGCG	525	0.0	39.428574	5
TAGGGTA	400	0.0	39.375	5
TTCGGGA	615	0.0	39.14634	4
TAGGGAA	1060	0.0	39.056602	5
>>END_MODULE