FastQCFastQC Report
Sat 14 Jan 2017
SRR2934245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304393
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC148591.1391505474193744No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG130240.9984720862500795No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC113460.8698298748919996RNA PCR Primer, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55180.42303201565785775No Hit
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC36900.2828902025693177Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC36510.27990030611939803RNA PCR Primer, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT36500.2798236421078617RNA PCR Primer, Index 13 (96% over 25bp)
GAATCAGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC35060.26878402444662RNA PCR Primer, Index 13 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC34620.2654108079390184No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT34020.26081096724683434No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTA25930.19878978191388638No Hit
GAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT25710.19710317366008556Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCAAAGCT23940.18353364361814267No Hit
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC23460.17985377106439548Illumina Single End Adapter 1 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19570.1500314705767357No Hit
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG17440.1337020361194824RNA PCR Primer, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA207.033636E-445.00000424
TACGGTC302.165727E-645.00000413
CGTTCGA207.033636E-445.00000437
TATCGGA207.033636E-445.00000433
GCGTAAA207.033636E-445.00000413
CCGATCG253.891097E-544.99999611
GTCGTAA253.891097E-544.9999969
CGTTTAT11100.042.770271
CGTTTTT28900.042.3529431
CTCGTAG750.042.01
TCGTCGG750.042.02
CGTTAGG3600.041.8752
TTAGGGA25850.041.77954
TAATACG650.041.538464
TTTAGGG42050.041.254463
CGATAGG603.6379788E-1241.2500042
TACGGCT17550.040.769237
TAGGGAT13150.040.551335
CGCTAGG1950.040.3846172
TGTAGGG14500.040.3448263
Produced by FastQC (version 0.11.5)