FastQCFastQC Report
Sat 14 Jan 2017
SRR2934243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937401
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC201132.1456132434251725No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCG163971.7491980486472702No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC130111.387986571381938No Hit
GAATCAGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTC43180.46063530975537686No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCT39750.424044779128676No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTC39570.4221245763552631No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC34460.3676121531767088TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATCGACTCGTA32370.3453164654187482No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATCGACT32040.3417960936674913No Hit
GAACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT30490.32526101422976933No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT20900.22295687757960575TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.21634284580451696No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC20200.21548942234966678TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC14110.15052256184919793TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC13920.14849568114392878TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGATCGACTCGT12200.13014707686465024No Hit
GAACTATCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT10540.11243854017650931No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT207.0326193E-445.00000436
GCGATAT351.2116107E-745.0000049
CCGTTAG207.0326193E-445.0000041
TCGTTAG1050.045.01
TCGTTAA253.890253E-545.022
GTTTACG253.890253E-545.01
CGTCATA253.890253E-545.038
TAGGGCG2750.044.181825
ATCAGTC4750.043.5789453
ATCCGGC1100.042.9545446
GCGATCT1950.042.6923079
TACGGCT23650.042.6215637
TTAGGGA22200.042.567574
CTTAGGG14900.042.1308753
ACGGCTG23950.042.087688
TAGGGAC7850.041.560515
GCCGATT1250.041.3999989
TCTTAGG9650.041.269432
TTTAGGG30000.041.1753
CTAGGGA11550.041.1038974
Produced by FastQC (version 0.11.5)