##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934241.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1846614
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42397328299255	33.0	31.0	34.0	31.0	34.0
2	32.58741187925576	34.0	31.0	34.0	31.0	34.0
3	32.63847073616901	34.0	31.0	34.0	31.0	34.0
4	36.085637821439676	37.0	35.0	37.0	35.0	37.0
5	35.21680221204865	37.0	35.0	37.0	35.0	37.0
6	26.202411548921432	37.0	0.0	37.0	0.0	37.0
7	31.1996215776551	37.0	19.0	37.0	17.0	37.0
8	34.84579072832763	37.0	32.0	37.0	32.0	37.0
9	37.389400816846404	39.0	37.0	39.0	35.0	39.0
10	37.42942271638794	39.0	37.0	39.0	35.0	39.0
11	37.42354926367936	39.0	37.0	39.0	35.0	39.0
12	37.17850184174928	39.0	37.0	39.0	34.0	39.0
13	37.19528986566765	39.0	37.0	39.0	34.0	39.0
14	38.12756591252963	40.0	38.0	41.0	33.0	41.0
15	38.316825281298634	40.0	38.0	41.0	33.0	41.0
16	38.48512141682019	40.0	38.0	41.0	34.0	41.0
17	38.50401166675873	40.0	38.0	41.0	34.0	41.0
18	38.4854051794257	40.0	38.0	41.0	34.0	41.0
19	38.509470306192846	40.0	38.0	41.0	34.0	41.0
20	38.42641342478721	40.0	38.0	41.0	34.0	41.0
21	38.3460977767958	40.0	37.0	41.0	34.0	41.0
22	38.40425503110016	40.0	37.0	41.0	34.0	41.0
23	38.37115769727729	40.0	37.0	41.0	34.0	41.0
24	38.32253194224673	40.0	37.0	41.0	34.0	41.0
25	38.221810297116775	40.0	37.0	41.0	34.0	41.0
26	38.19171142426084	40.0	37.0	41.0	34.0	41.0
27	38.16775027157814	40.0	37.0	41.0	34.0	41.0
28	38.0856096617918	40.0	37.0	41.0	34.0	41.0
29	38.0709287376788	40.0	37.0	41.0	34.0	41.0
30	37.833430809037516	40.0	36.0	41.0	34.0	41.0
31	37.81312337066653	40.0	36.0	41.0	33.0	41.0
32	37.78026214466044	40.0	36.0	41.0	33.0	41.0
33	37.734987387726946	40.0	36.0	41.0	33.0	41.0
34	37.62987771131379	40.0	36.0	41.0	33.0	41.0
35	37.619159174575735	40.0	36.0	41.0	33.0	41.0
36	37.57346202292412	40.0	36.0	41.0	33.0	41.0
37	37.46575299439948	40.0	36.0	41.0	33.0	41.0
38	37.354854885753056	40.0	36.0	41.0	32.0	41.0
39	37.36259987198191	40.0	36.0	41.0	32.0	41.0
40	37.34738012383747	40.0	36.0	41.0	33.0	41.0
41	37.256928085674645	40.0	36.0	41.0	32.0	41.0
42	37.23872828864072	40.0	35.0	41.0	32.0	41.0
43	37.17821320535857	40.0	35.0	41.0	32.0	41.0
44	37.06258481740093	39.0	35.0	41.0	32.0	41.0
45	37.04551682159888	39.0	35.0	41.0	32.0	41.0
46	37.00087024142566	39.0	35.0	41.0	32.0	41.0
47	36.90413102034318	39.0	35.0	41.0	31.0	41.0
48	36.8431133956528	39.0	35.0	41.0	31.0	41.0
49	36.815413508183084	39.0	35.0	41.0	31.0	41.0
50	36.740867338815804	39.0	35.0	41.0	31.0	41.0
51	35.737719956634145	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	17.0
11	15.0
12	8.0
13	12.0
14	24.0
15	29.0
16	78.0
17	122.0
18	264.0
19	572.0
20	928.0
21	1604.0
22	2452.0
23	3804.0
24	5691.0
25	8348.0
26	11082.0
27	13486.0
28	16013.0
29	19469.0
30	24702.0
31	33367.0
32	48542.0
33	78945.0
34	113730.0
35	126049.0
36	154981.0
37	273500.0
38	414985.0
39	493440.0
40	337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.008111061651217	3.971701720012953	57.387683619857754	15.63250359847808
2	19.726104101885937	4.87205230762899	54.748637235502386	20.653206354982686
3	20.36137492729937	5.075018385000872	53.33756811114829	21.226038576551463
4	18.987400723702947	4.3935007532705805	53.172563405237916	23.446535117788557
5	18.546973000313006	7.292590655112546	50.83368803659021	23.326748307984236
6	16.008434897601774	30.975287742863422	39.85369979865852	13.162577560876285
7	86.09644462784317	1.41399339547951	8.782777559359996	3.7067844173173174
8	88.2788714912808	2.2556961010801393	6.0162546152038265	3.4491777924352354
9	83.28881942842413	4.297649644159526	7.807641445369741	4.605889482046599
10	40.31952535830444	34.37984332405148	14.112857370300452	11.187773947343626
11	26.947104267594636	23.564372413509265	31.038863563256857	18.44965975563924
12	26.00115671168961	21.782895613268394	32.01654487618961	20.199402798852386
13	25.793316849108695	22.003082398378872	33.84768013239366	18.355920620118766
14	19.956200916921457	24.91554813296119	35.00444597517402	20.12380497494333
15	17.817583967196178	26.18506087357726	36.67182204835445	19.325533110872115
16	24.09653560516708	26.17184750034387	30.563723658544774	19.167893235944273
17	24.289808265289878	25.300793777151043	29.57938150582634	20.83001645173274
18	23.99044954711705	24.42714070184673	31.576008846461683	20.006400904574534
19	23.149721598558227	26.496604054772682	30.162719442179036	20.190954904490056
20	22.88799933283296	30.370505151590965	29.812727511001214	16.92876800457486
21	23.549642751544177	26.958097360899462	32.384948884823785	17.10731100273257
22	21.613991879190777	23.385937721689533	33.875298248578204	21.124772150541478
23	20.26969361220049	25.51865197599498	32.90530668564194	21.306347726162585
24	20.747053796841136	27.042413845015794	30.803513890829375	21.407018467313687
25	22.329571854215338	27.31919069172009	28.113617680793062	22.23761977327151
26	20.387260142076254	27.274514327303915	28.343443729983637	23.994781800636193
27	19.847353047252973	26.274955134099493	32.09463374587217	21.78305807277536
28	17.95193797945862	26.45815530479028	31.622959643975406	23.966947071775692
29	21.134140648776626	26.675796890958264	30.38588465158393	21.804177808681185
30	24.635630402455522	24.76115744817271	30.613598727183916	19.98961342218785
31	22.53692433827535	25.992113132468397	30.83091539433796	20.640047134918287
32	24.232947437851116	26.978134033425498	28.29616801345598	20.492750515267403
33	24.623283479925963	25.850665055068355	28.23524569834302	21.29080576666266
34	20.768498451760898	25.521522093951415	31.298311395884575	22.41166805840311
35	20.544358485314202	27.636095036645447	29.33222644255919	22.487320035481158
36	24.825708025607952	26.9143957535251	27.67140290282647	20.58849331804048
37	21.083399129433655	28.285933064517003	29.70994479625953	20.92072300978981
38	21.313929169821087	27.83657006824382	30.1583330354909	20.691167726444185
39	21.22522627901662	26.647149864562923	28.549658997494877	23.57796485892558
40	23.310285744611488	24.3357301525928	29.629635646648406	22.724348456147307
41	19.98750144859727	25.367835400359795	29.096010319427883	25.548652831615055
42	20.30007354000349	25.64558700410589	31.355388835999292	22.698950619891324
43	20.831641046802417	25.59679499884654	30.62096355816646	22.950600396184583
44	21.495504745442197	25.14445357827895	29.356107990083473	24.003933686195385
45	22.135432743388712	23.341044744597408	27.957548247765914	26.56597426424797
46	22.73718275719777	24.337787973014393	29.635321729392285	23.289707540395558
47	19.64509096107795	25.406229997173202	32.434607340786975	22.514071700961868
48	19.540900263942547	27.383199737465436	30.85528432038314	22.220615678208873
49	22.469936868235592	24.322408473021433	31.296416034969948	21.911238623773023
50	21.0700232966933	24.653609254559967	29.453854460109152	24.822512988637584
51	19.67124694169978	24.526674226449057	28.355628193006226	27.446450638844933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	867.5
2	1515.0
3	5047.0
4	8579.0
5	5711.5
6	2844.0
7	2870.0
8	2896.0
9	3019.5
10	3143.0
11	3234.0
12	3325.0
13	3426.0
14	3527.0
15	3685.0
16	3843.0
17	3932.0
18	4021.0
19	4588.0
20	5155.0
21	5918.5
22	6682.0
23	8642.5
24	10603.0
25	12713.0
26	18639.0
27	22455.0
28	27374.5
29	32294.0
30	37095.0
31	41896.0
32	48247.5
33	54599.0
34	60685.0
35	66771.0
36	71543.5
37	76316.0
38	80317.0
39	84318.0
40	92723.0
41	101128.0
42	107950.5
43	114773.0
44	124247.0
45	133721.0
46	156995.5
47	180270.0
48	188912.0
49	197554.0
50	188767.0
51	179980.0
52	157655.5
53	135331.0
54	117834.0
55	100337.0
56	90005.5
57	79674.0
58	70559.0
59	61444.0
60	50647.5
61	39851.0
62	33865.5
63	27880.0
64	23317.5
65	18755.0
66	14661.5
67	10568.0
68	8624.0
69	6680.0
70	5297.0
71	3914.0
72	3123.5
73	2333.0
74	1835.5
75	1026.0
76	714.0
77	505.5
78	297.0
79	236.0
80	175.0
81	109.5
82	44.0
83	27.5
84	11.0
85	8.0
86	5.0
87	5.0
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1846614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.83566684369246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.90223071654273	23.609576931267075
2	12.016034154340517	7.8910898854871
3	4.42689569701268	4.360802167766522
4	2.103754938437532	2.763127851172301
5	1.2831144836307788	2.106595985340837
6	0.832952575260465	1.6410331954708974
7	0.5966635312216146	1.3714291450281821
8	0.47615904972927353	1.250799993721569
9	0.3866964636129099	1.1427692623944794
>10	5.734385640300263	43.979375332013504
>50	0.18147700728607447	3.8178634034570216
>100	0.05357441102381975	3.0179708145798
>500	0.00351948286529599	0.7943779918609827
>1k	0.0025418487360471037	2.2531880404397633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	4161	0.225331336164461	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	3996	0.21639606328122718	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3888	0.21054752103038318	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3688	0.19971688723252395	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	3177	0.17204461787899367	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	3146	0.17036586964032546	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3098	0.16776651752883928	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2868	0.15531128866130117	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0830633797859216E-4	0.0	0.0	0.237949024538967	0.0
2	1.0830633797859216E-4	0.0	0.0	0.8182543834282638	0.0
3	1.0830633797859216E-4	0.0	0.0	1.164672205452791	0.0
4	1.0830633797859216E-4	0.0	0.0	1.6040710186319393	0.0
5	1.0830633797859216E-4	0.0	0.0	2.4293111608598226	0.0
6	1.0830633797859216E-4	0.0	0.0	2.9571420989984913	0.0
7	1.0830633797859216E-4	0.0	0.0	3.6130452817968455	0.0
8	1.0830633797859216E-4	0.0	0.0	4.654302415123031	0.0
9	1.0830633797859216E-4	0.0	0.0	5.150182983558015	0.0
10	1.6245950696788827E-4	0.0	0.0	6.172215741893	0.0
11	1.6245950696788827E-4	0.0	0.0	7.947194161855157	0.0
12	1.6245950696788827E-4	0.0	0.0	9.780658004325755	0.0
13	1.6245950696788827E-4	0.0	0.0	10.316990989995743	0.0
14	1.6245950696788827E-4	0.0	0.0	10.532953827925056	0.0
15	2.1661267595718433E-4	0.0	0.0	10.805073502096269	0.0
16	2.1661267595718433E-4	0.0	0.0	11.351641436705235	0.0
17	2.1661267595718433E-4	0.0	0.0	12.167621387035949	0.0
18	2.1661267595718433E-4	0.0	0.0	13.024216214108634	0.0
19	2.1661267595718433E-4	0.0	0.0	13.7630278986296	0.0
20	2.1661267595718433E-4	0.0	0.0	14.49766978913839	0.0
21	2.1661267595718433E-4	5.415316898929608E-5	0.0	15.543475788659677	0.0
22	2.1661267595718433E-4	5.415316898929608E-5	0.0	16.70971843601316	0.0
23	2.1661267595718433E-4	5.415316898929608E-5	0.0	17.790832301715465	0.0
24	2.1661267595718433E-4	5.415316898929608E-5	0.0	18.558236859462777	0.0
25	2.1661267595718433E-4	5.415316898929608E-5	0.0	19.247931619710453	0.0
26	2.1661267595718433E-4	5.415316898929608E-5	0.0	19.86982661238353	0.0
27	2.1661267595718433E-4	5.415316898929608E-5	0.0	20.55091101876191	0.0
28	2.1661267595718433E-4	5.415316898929608E-5	0.0	21.1924636117781	0.0
29	2.707658449464804E-4	5.415316898929608E-5	0.0	21.951095356149146	0.0
30	3.2491901393577653E-4	5.415316898929608E-5	0.0	22.84711369024604	0.0
31	3.2491901393577653E-4	5.415316898929608E-5	0.0	23.572224623012715	0.0
32	3.2491901393577653E-4	5.415316898929608E-5	0.0	24.24437375650786	0.0
33	3.2491901393577653E-4	5.415316898929608E-5	0.0	24.91820163824167	0.0
34	3.2491901393577653E-4	5.415316898929608E-5	0.0	25.648023896710413	0.0
35	3.2491901393577653E-4	5.415316898929608E-5	0.0	26.456314097044643	0.0
36	3.2491901393577653E-4	5.415316898929608E-5	0.0	27.139618783351583	0.0
37	3.2491901393577653E-4	5.415316898929608E-5	0.0	27.854765533024228	0.0
38	3.2491901393577653E-4	5.415316898929608E-5	0.0	28.696576544962834	0.0
39	3.2491901393577653E-4	5.415316898929608E-5	0.0	30.202251255541224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAG	35	1.2124656E-7	45.000004	1
CGAACGT	20	7.034401E-4	45.0	37
GCGAAAT	20	7.034401E-4	45.0	9
ACGTCTA	20	7.034401E-4	45.0	44
TCGAGTA	20	7.034401E-4	45.0	11
CCGAACG	20	7.034401E-4	45.0	36
CGATAGG	50	2.1827873E-11	45.0	2
CGTTTAT	840	0.0	43.392857	1
TTAGGGA	5860	0.0	42.235493	4
TAGGGAT	4010	0.0	42.194515	5
CGTTAGG	540	0.0	42.083332	2
TAGGGGT	2365	0.0	41.575054	5
TAGGGAC	2420	0.0	41.559917	5
TTTAGGG	7970	0.0	41.160603	3
TAGGGCG	690	0.0	41.086956	5
TATCGCG	55	6.184564E-11	40.909092	1
AGGGCGA	1025	0.0	40.82927	6
CTAGGGA	2735	0.0	40.72212	4
TCGTTAG	360	0.0	40.625	1
GCGCGAC	100	0.0	40.5	9
>>END_MODULE