##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934240.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303519
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21046030015673	33.0	31.0	34.0	30.0	34.0
2	32.381744339744955	34.0	31.0	34.0	30.0	34.0
3	32.40182920233614	34.0	31.0	34.0	30.0	34.0
4	35.94921746441747	37.0	35.0	37.0	35.0	37.0
5	35.091335070681744	37.0	35.0	37.0	35.0	37.0
6	26.062914311183803	36.0	0.0	37.0	0.0	37.0
7	31.09696061200489	37.0	19.0	37.0	17.0	37.0
8	34.74719969559324	37.0	32.0	37.0	32.0	37.0
9	37.29934814912556	39.0	37.0	39.0	35.0	39.0
10	37.20443046860076	39.0	37.0	39.0	34.0	39.0
11	37.272526906013645	39.0	37.0	39.0	34.0	39.0
12	37.17082604856546	39.0	37.0	39.0	34.0	39.0
13	37.25262385895411	39.0	37.0	39.0	34.0	39.0
14	38.4156893762193	40.0	38.0	41.0	34.0	41.0
15	38.50466774937688	40.0	38.0	41.0	34.0	41.0
16	38.56965567820645	40.0	38.0	41.0	34.0	41.0
17	38.568277869367456	40.0	38.0	41.0	34.0	41.0
18	38.533561075826285	40.0	38.0	41.0	35.0	41.0
19	38.50641686082059	40.0	38.0	41.0	34.0	41.0
20	38.40071452736784	40.0	37.0	41.0	34.0	41.0
21	38.302834097546715	40.0	37.0	41.0	34.0	41.0
22	38.30393266227804	40.0	37.0	41.0	34.0	41.0
23	38.221379205059534	40.0	37.0	41.0	34.0	41.0
24	38.1644893553527	40.0	37.0	41.0	34.0	41.0
25	38.033185553873786	40.0	37.0	41.0	34.0	41.0
26	38.00312998889928	40.0	37.0	41.0	34.0	41.0
27	37.99381903907806	40.0	37.0	41.0	34.0	41.0
28	37.949539669157105	40.0	37.0	41.0	34.0	41.0
29	37.93573779898874	40.0	37.0	41.0	34.0	41.0
30	37.68066748547585	40.0	36.0	41.0	33.0	41.0
31	37.68891132388558	40.0	36.0	41.0	33.0	41.0
32	37.52507021378285	40.0	36.0	41.0	33.0	41.0
33	37.36495977427256	40.0	36.0	41.0	33.0	41.0
34	37.24555299922748	40.0	36.0	41.0	32.0	41.0
35	37.17131779437047	40.0	36.0	41.0	32.0	41.0
36	37.073370622138995	40.0	36.0	41.0	32.0	41.0
37	37.0476809313865	40.0	36.0	41.0	32.0	41.0
38	36.978810435444366	40.0	36.0	41.0	31.0	41.0
39	36.901552643267955	40.0	36.0	41.0	31.0	41.0
40	36.84328958764698	39.0	36.0	41.0	31.0	41.0
41	36.77030407688726	39.0	35.0	41.0	31.0	41.0
42	36.69139920476802	39.0	35.0	41.0	31.0	41.0
43	36.63382275210411	39.0	35.0	41.0	31.0	41.0
44	36.565231500269654	39.0	35.0	41.0	31.0	41.0
45	36.552579594160115	39.0	35.0	41.0	30.0	41.0
46	36.47285079849239	39.0	35.0	41.0	30.0	41.0
47	36.37847396163769	39.0	35.0	41.0	30.0	41.0
48	36.354662264224764	39.0	35.0	41.0	30.0	41.0
49	36.34707127398987	39.0	35.0	41.0	30.0	41.0
50	36.28317193688776	39.0	35.0	40.0	30.0	41.0
51	35.317170674152045	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	3.0
12	5.0
13	6.0
14	11.0
15	17.0
16	41.0
17	68.0
18	161.0
19	360.0
20	720.0
21	1271.0
22	2113.0
23	3514.0
24	5978.0
25	9814.0
26	13076.0
27	13679.0
28	13347.0
29	14522.0
30	17836.0
31	23549.0
32	32124.0
33	48393.0
34	72539.0
35	98120.0
36	126761.0
37	212356.0
38	297030.0
39	295973.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.52863211046406	4.655474910607364	58.228840546244435	14.58705243268414
2	21.86205187649739	4.741472889923354	52.94107719181692	20.455398041762336
3	18.668619329676055	4.6194953813484885	55.32623613464782	21.38564915432763
4	16.855450515105648	4.097830564801893	55.42496887272069	23.621750047371766
5	16.47563249941121	7.28735062549913	53.74666575631042	22.49035111877924
6	14.24160292255042	30.99103273523439	42.544757690528485	12.222606651686704
7	81.5939008177096	1.1266425729122476	12.962987114111876	4.316469495266275
8	83.3568977513945	1.6683301125645273	10.289608360138978	4.685163775902001
9	78.72528133460271	3.5144865552400844	12.293184832748889	5.467047277408308
10	35.98067999008837	26.422322958084997	22.29511038964526	15.30188666218137
11	23.60648367994636	26.095055001116208	28.999270436410978	21.299190882526453
12	20.858767689615572	21.99047347986489	35.00225159740671	22.148507233112827
13	21.885219931585194	23.70299167100748	35.93434387991276	18.47744451749457
14	20.228320415736174	25.76594587420667	34.18807090652303	19.817662803534127
15	17.191157167636224	26.221712149957156	35.932502709972006	20.654627972434618
16	18.466167351607456	26.036137563012122	33.581558841873424	21.916136243506998
17	18.65435026263522	26.432986400658525	30.833689420714233	24.07897391599202
18	19.39127853142148	25.905337781804484	33.22605961247976	21.477324074294277
19	19.906422537761244	28.745495846243895	31.235217898626715	20.11286371736814
20	20.484780045400182	28.463566699066146	31.934632329870144	19.11702092566353
21	20.243816929404172	28.608328685657824	32.28545191899773	18.862402465940274
22	19.16166929672678	25.37400682306894	31.868043350346255	23.596280529858024
23	18.07100625307341	27.501785551265463	30.13964506846467	24.287563127196456
24	19.382532974202906	26.27295804664144	31.238363230608833	23.10614574854682
25	17.694640431017884	28.914346472893758	28.93736109715317	24.453651998935193
26	17.263653234053358	29.259258975128095	30.502508977621346	22.974578813197198
27	18.04837520588499	27.4417940973626	31.470734220214663	23.03909647653774
28	15.857766553460287	26.446641744385772	33.01869784790249	24.676893854251453
29	16.275788845425346	25.487468920667823	32.04364493344554	26.19309730046129
30	18.080595679848166	27.73239208634473	30.894524744173275	23.292487489633828
31	17.25651870053294	29.23877595953722	29.00655840075979	24.498146939170045
32	16.099036531113086	31.119684484844484	26.848246937712457	25.933032046329973
33	17.246238835030407	28.636790104325293	26.987715560724467	27.12925549991983
34	15.281173500347906	28.123333837097885	28.111366232482993	28.484126430071218
35	14.573627235199488	29.405862131660527	25.96233733455362	30.058173298586365
36	16.132561167117625	31.05148448162244	26.525275043938752	26.29067930732118
37	14.876959982938493	28.314431933865176	30.344935516858595	26.46367256633774
38	15.418877668833368	28.45274982566422	28.796741742928184	27.331630762574232
39	16.524730364497948	29.25726437435895	27.814017287051435	26.40398797409167
40	17.503772480493186	27.71536126439277	27.501171827951875	27.279694427162166
41	15.839278138638562	28.07377567952596	25.909863991242165	30.177082190593307
42	16.999138485898555	28.490033516964463	26.96585166767803	27.544976329458947
43	17.27730857778061	27.881373420717303	28.946029938957548	25.89528806254454
44	17.003511264507846	26.527576506364692	27.63174146291692	28.83717076621054
45	18.061953834198043	26.11983407990217	25.86628963597769	29.951922449922098
46	18.753466577779072	26.714301824522696	27.343751798017518	27.18847979968071
47	14.601321499724977	27.64961615442506	30.030555749475074	27.718506596374894
48	14.977150313881118	27.007968430072747	29.710115464369906	28.304765791676225
49	17.754401738678148	24.98828172048125	30.537951498980835	26.719365041859767
50	17.175200361483032	24.207855811844706	28.003964652605756	30.612979174066506
51	15.529808157763714	24.40892691245774	25.62709097450824	34.43417395527031
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	326.0
2	575.0
3	7275.0
4	13975.0
5	9163.5
6	4352.0
7	4487.0
8	4622.0
9	5092.0
10	5562.0
11	6045.5
12	6529.0
13	6608.5
14	6688.0
15	6555.0
16	6422.0
17	6293.5
18	6165.0
19	6165.0
20	6165.0
21	6045.5
22	5926.0
23	6401.0
24	6876.0
25	7200.0
26	8662.0
27	9800.0
28	12185.5
29	14571.0
30	16160.5
31	17750.0
32	20588.0
33	23426.0
34	28596.0
35	33766.0
36	37067.0
37	40368.0
38	46431.5
39	52495.0
40	61415.5
41	70336.0
42	80632.5
43	90929.0
44	104180.5
45	117432.0
46	141461.0
47	165490.0
48	175883.0
49	186276.0
50	169571.5
51	152867.0
52	123606.5
53	94346.0
54	76084.0
55	57822.0
56	47222.0
57	36622.0
58	30334.5
59	24047.0
60	19262.5
61	14478.0
62	11797.0
63	9116.0
64	6892.0
65	4668.0
66	3741.0
67	2814.0
68	2024.0
69	1234.0
70	978.0
71	722.0
72	527.5
73	333.0
74	261.0
75	134.5
76	80.0
77	58.0
78	36.0
79	31.0
80	26.0
81	16.0
82	6.0
83	7.5
84	9.0
85	5.5
86	2.0
87	2.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1303519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.455084307497934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97904908274052	30.983316814887786
2	12.731957935440224	10.81072695097264
3	4.75611317066333	6.057635569095387
4	2.309648921869798	3.9222535879481586
5	1.3569839065606426	2.880543307847499
6	0.9411717530722544	2.3974515674710912
7	0.6682284657233916	1.9858787094269605
8	0.5140820293652427	1.7460316718136808
9	0.42684953166667056	1.630973956817206
>10	3.135519058417704	25.155285772676134
>50	0.11488404655356843	3.355269069353059
>100	0.05838284191433155	4.447087542222257
>500	0.003660969303658379	1.0301522074447849
>1k	0.0030829215188702143	2.616895824359459
>5k	3.853651898587768E-4	0.9804974476640348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6973	0.5349365832028532	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	5013	0.3845743713747172	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	3935	0.3018751548692424	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	3812	0.2924391589228849	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	3202	0.24564275626208745	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2603	0.19969022315746834	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	2345	0.179897646294377	No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	1942	0.14898133437257147	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	1918	0.1471401644318188	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	1833	0.14061935422498636	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	1663	0.1275777338113215	No Hit
TCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	1437	0.11024005020256705	No Hit
CTGTCACTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	1415	0.10855231109021042	No Hit
TCTGTATCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1392	0.10678785656365578	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	1330	0.102031500883378	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5343082839605713E-4	0.0	0.0	0.40290935536804606	0.0
2	1.5343082839605713E-4	0.0	0.0	1.3119102982004864	0.0
3	1.5343082839605713E-4	0.0	0.0	1.8983229243302169	0.0
4	2.301462425940857E-4	0.0	0.0	2.688645121398307	0.0
5	2.301462425940857E-4	0.0	0.0	4.129974323350868	0.0
6	2.301462425940857E-4	0.0	0.0	5.101344897926306	0.0
7	2.301462425940857E-4	0.0	0.0	6.222003668531107	0.0
8	2.301462425940857E-4	0.0	0.0	7.959607800116454	0.0
9	2.301462425940857E-4	0.0	0.0	8.685719195500795	0.0
10	2.301462425940857E-4	0.0	0.0	10.331648407119497	0.0
11	2.301462425940857E-4	0.0	0.0	13.544720099975528	0.0
12	2.301462425940857E-4	0.0	0.0	16.44954925858388	0.0
13	2.301462425940857E-4	0.0	0.0	17.41524289250866	0.0
14	2.301462425940857E-4	0.0	0.0	17.737294201311986	0.0
15	2.301462425940857E-4	0.0	0.0	18.128926390792923	0.0
16	2.301462425940857E-4	0.0	0.0	19.070071092174338	0.0
17	2.301462425940857E-4	0.0	0.0	20.583819645129836	0.0
18	2.301462425940857E-4	0.0	0.0	22.17489733559695	0.0
19	2.301462425940857E-4	0.0	0.0	23.37426612116893	0.0
20	3.0686165679211425E-4	0.0	0.0	24.51909024724611	0.0
21	3.0686165679211425E-4	0.0	0.0	26.286536674954487	0.0
22	3.0686165679211425E-4	0.0	0.0	28.226209207537444	0.0
23	3.0686165679211425E-4	0.0	0.0	30.209225949142283	0.0
24	3.0686165679211425E-4	0.0	0.0	31.563636586808478	0.0
25	3.0686165679211425E-4	0.0	0.0	32.73254935294384	0.0
26	3.0686165679211425E-4	0.0	0.0	33.7419707729615	0.0
27	3.0686165679211425E-4	0.0	0.0	34.73505180975498	0.0
28	3.0686165679211425E-4	0.0	0.0	35.81950090485831	0.0
29	3.0686165679211425E-4	0.0	0.0	36.902645837920275	0.0
30	3.0686165679211425E-4	0.0	0.0	38.0963376828416	0.0
31	3.0686165679211425E-4	0.0	0.0	39.17779487679121	0.0
32	3.0686165679211425E-4	7.671541419802856E-5	0.0	40.172256791040255	0.0
33	3.0686165679211425E-4	7.671541419802856E-5	0.0	41.15613197812997	0.0
34	3.0686165679211425E-4	7.671541419802856E-5	0.0	42.09620266371261	0.0
35	3.0686165679211425E-4	7.671541419802856E-5	0.0	43.113372340564275	0.0
36	3.0686165679211425E-4	7.671541419802856E-5	0.0	44.1046889228312	0.0
37	3.0686165679211425E-4	7.671541419802856E-5	0.0	45.043378730958274	0.0
38	3.0686165679211425E-4	7.671541419802856E-5	0.0	45.97999722290201	0.0
39	3.0686165679211425E-4	7.671541419802856E-5	0.0	46.99287083655858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.0336345E-4	45.000004	40
CGCATCG	20	7.0336345E-4	45.000004	21
ACCTACG	20	7.0336345E-4	45.000004	19
CGCTCCG	20	7.0336345E-4	45.000004	12
TACGAAG	20	7.0336345E-4	45.000004	25
CCGACAA	20	7.0336345E-4	45.000004	16
CCGGTTG	20	7.0336345E-4	45.000004	1
TCGACGG	20	7.0336345E-4	45.000004	2
CCGCATC	20	7.0336345E-4	45.000004	20
CGACGTA	25	3.891094E-5	45.0	28
CAACGGT	35	1.2121018E-7	45.0	21
GCACCGT	25	3.891094E-5	45.0	9
ATCGTAG	25	3.891094E-5	45.0	1
GACGGTT	50	2.1827873E-11	45.0	9
CGAATAA	25	3.891094E-5	45.0	13
TTCGCAG	100	0.0	45.0	1
GCCGTTG	50	2.1827873E-11	45.0	1
AGTGCGA	30	2.165727E-6	44.999996	25
CGTTTAT	1645	0.0	44.179333	1
CGTTTTT	4020	0.0	43.768658	1
>>END_MODULE