SRR2934238.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586064
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	2123	0.3622471265936826	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2114	0.3607114581342652	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1730	0.2951896038657894	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1587	0.27078953834393515	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1548	0.26413497501979305	No Hit
GCTGTATCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	680	0.1160282836004259	TruSeq Adapter, Index 27 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	627	0.10698490267274563	TruSeq Adapter, Index 27 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	593	0.10118348849272435	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	591	0.10084222883507603	TruSeq Adapter, Index 27 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAA	20	7.030454E-4	45.0	17
TCCGCAC	20	7.030454E-4	45.0	31
TTAATCG	25	3.888458E-5	45.0	20
ACGTTAC	20	7.030454E-4	45.0	12
CGTGCGA	20	7.030454E-4	45.0	31
CCCTACT	20	7.030454E-4	45.0	23
ATAGCGT	20	7.030454E-4	45.0	17
CGTTACG	20	7.030454E-4	45.0	13
GCCGATT	55	1.8189894E-12	45.0	9
AATTGCG	20	7.030454E-4	45.0	1
GTCGTTG	20	7.030454E-4	45.0	1
TTGCACG	20	7.030454E-4	45.0	2
TGTGTCG	40	6.8048394E-9	45.0	1
CGATAGC	20	7.030454E-4	45.0	15
ACCTTCG	20	7.030454E-4	45.0	23
GTAGTAC	40	6.8048394E-9	45.0	9
ACGGTGC	20	7.030454E-4	45.0	41
ACGAACA	30	2.1636752E-6	44.999996	25
CGTTAGG	145	0.0	43.448273	2
CTAGGGA	655	0.0	42.938927	4