##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586064
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.302439665292525	33.0	31.0	34.0	31.0	34.0
2	32.476756804717574	34.0	31.0	34.0	31.0	34.0
3	32.530872054929155	34.0	31.0	34.0	31.0	34.0
4	36.0187914630484	37.0	35.0	37.0	35.0	37.0
5	35.12520816839117	37.0	35.0	37.0	35.0	37.0
6	25.936680976821645	36.0	0.0	37.0	0.0	37.0
7	31.006067596712988	37.0	19.0	37.0	17.0	37.0
8	34.70519772584564	37.0	32.0	37.0	32.0	37.0
9	37.22111236998007	39.0	37.0	39.0	34.0	39.0
10	37.333106964427095	39.0	37.0	39.0	34.0	39.0
11	37.40412480548199	39.0	37.0	39.0	35.0	39.0
12	36.98717034317071	39.0	35.0	39.0	34.0	39.0
13	36.895675216358626	39.0	35.0	39.0	33.0	39.0
14	37.742309713614894	40.0	36.0	41.0	33.0	41.0
15	37.978831663436075	40.0	37.0	41.0	33.0	41.0
16	38.14525546697971	40.0	37.0	41.0	33.0	41.0
17	38.191296513691334	40.0	37.0	41.0	34.0	41.0
18	38.172588659259056	40.0	36.0	41.0	34.0	41.0
19	38.19232711785744	40.0	37.0	41.0	34.0	41.0
20	38.10512162494199	40.0	36.0	41.0	34.0	41.0
21	37.9888561658795	40.0	36.0	41.0	34.0	41.0
22	38.03657791640504	40.0	36.0	41.0	34.0	41.0
23	38.01540446094624	40.0	36.0	41.0	34.0	41.0
24	37.96576653744301	40.0	36.0	41.0	34.0	41.0
25	37.85086611701111	40.0	35.0	41.0	34.0	41.0
26	37.826626443528355	40.0	35.0	41.0	34.0	41.0
27	37.79022086325043	40.0	35.0	41.0	34.0	41.0
28	37.74898645881679	40.0	35.0	41.0	34.0	41.0
29	37.73324926970433	40.0	35.0	41.0	34.0	41.0
30	37.49849675120806	40.0	35.0	41.0	33.0	41.0
31	37.46992990526632	40.0	35.0	41.0	33.0	41.0
32	37.4235254170193	39.0	35.0	41.0	33.0	41.0
33	37.37477135602938	39.0	35.0	41.0	33.0	41.0
34	37.27844057986841	40.0	35.0	41.0	33.0	41.0
35	37.22814914412078	40.0	35.0	41.0	32.0	41.0
36	37.17035682109804	39.0	35.0	41.0	32.0	41.0
37	37.12247638483169	39.0	35.0	41.0	32.0	41.0
38	37.04593866881433	39.0	35.0	41.0	32.0	41.0
39	37.00230009009255	39.0	35.0	41.0	32.0	41.0
40	36.907733626361626	39.0	35.0	41.0	32.0	41.0
41	36.79246293920118	39.0	35.0	41.0	31.0	41.0
42	36.774599702421575	39.0	35.0	41.0	31.0	41.0
43	36.7227623604248	39.0	35.0	41.0	31.0	41.0
44	36.596279587212315	39.0	35.0	41.0	31.0	41.0
45	36.52200442272516	39.0	35.0	41.0	31.0	41.0
46	36.46624259466543	38.0	35.0	40.0	31.0	41.0
47	36.36880101831882	38.0	35.0	40.0	31.0	41.0
48	36.370058560157254	38.0	35.0	40.0	31.0	41.0
49	36.36663572579104	38.0	35.0	40.0	31.0	41.0
50	36.275874307242894	38.0	35.0	40.0	31.0	41.0
51	35.30998662262142	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	17.0
11	2.0
12	8.0
13	11.0
14	5.0
15	16.0
16	38.0
17	60.0
18	124.0
19	182.0
20	329.0
21	580.0
22	824.0
23	1284.0
24	1968.0
25	2890.0
26	3837.0
27	4821.0
28	5765.0
29	7083.0
30	8916.0
31	11993.0
32	17367.0
33	29732.0
34	43551.0
35	51010.0
36	52776.0
37	88786.0
38	121455.0
39	130556.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.360943514701468	4.835990608534221	52.59084332086598	16.212222555898332
2	21.466426874880558	4.575097600262088	50.567344180840315	23.391131344017037
3	21.73295066750389	4.78667858800404	50.53594829233668	22.944422452155397
4	19.492581015042727	4.976589587485326	49.66454175653171	25.86628764094024
5	17.350494143984275	8.456414316525157	48.8994376040842	25.293653935406375
6	16.0926110458926	31.545530863523435	38.6329820633924	13.72887602719157
7	82.30056785607033	2.7321248191323813	9.833397035136095	5.1339102896611974
8	83.25012285347675	2.714038057277021	8.62158399082694	5.414255098419285
9	77.96725272325207	4.959355974774086	10.910071255016517	6.163320046957329
10	41.51201916514237	25.68286057495427	17.88456550820388	14.920554751699472
11	33.71082339130197	22.38253842583745	24.888749351606652	19.017888831253924
12	30.675318736520246	21.68159108902782	29.264210052144474	18.37888012230746
13	26.6720016926479	23.918206885254854	30.570893281279858	18.838898140817385
14	19.8568756995823	26.30941330639657	33.36512735810423	20.468583635916897
15	17.90452919817631	22.91746293920118	37.71857681072374	21.45943105189877
16	23.527123317589886	24.464392967320975	31.107353463103003	20.90113025198613
17	24.0251917879276	22.883507603265173	28.84309563460646	24.24820497420077
18	26.100050506429334	21.348692292991892	31.42028856916651	21.13096863141227
19	25.49840972999536	24.536910644571243	28.504224794561683	21.460454830871715
20	25.86424008299435	28.05273826749297	27.637425264134976	18.445596385377705
21	24.587587703732016	26.485332659914274	30.641363400584236	18.285716235769474
22	22.896646100084634	23.40153976357531	31.635623413142593	22.066190723197465
23	21.17123727101477	24.80411695650987	31.18959704059625	22.835048731879112
24	22.415640612629335	24.58451636681318	30.589662562450513	22.410180458106964
25	22.214297414616833	25.31993092904529	27.197712195255125	25.26805946108275
26	20.58904829506675	25.015356684594174	27.827506893445086	26.56808812689399
27	19.452483005269052	24.455349586393297	33.31513281825876	22.777034590078898
28	16.413224494253186	24.886189904174287	32.24801386879248	26.45257173278004
29	18.748976221027057	24.94130333888449	31.392987796554646	24.916732643533813
30	22.484575063474296	21.980875808785388	33.87531054628846	21.659238581451856
31	23.182792323022742	23.58138360315597	31.54689590215403	21.688928171667257
32	25.270107019028636	23.74075186327773	28.2182492014524	22.77089191624123
33	22.994416992000875	23.16385241202326	29.09682219006798	24.744908405907886
34	19.84970924677168	22.339027819487292	33.134947719020445	24.676315214720574
35	19.40743673045947	24.65959349149581	29.697097927871358	26.23587185017336
36	21.700360370198478	24.67887466215294	29.907655136640365	23.713109831008218
37	19.568170029211824	26.43994512544705	31.168097682164404	22.823787163176718
38	21.090188102323296	25.812368615031804	28.85145649621884	24.245986786426055
39	20.06726227852248	22.888967757787544	30.491891670534276	26.551878293155696
40	24.44852439324033	21.22464440743673	31.075275055284063	23.251556144038876
41	20.561235632968415	21.855974774086107	28.750443637554945	28.832345955390537
42	21.90904065085042	21.425134456305113	29.552403833028475	27.113421059815995
43	21.648659532064755	20.568743345436676	30.895601845532227	26.886995276966335
44	20.915292527778536	21.45038767097109	29.934614649594582	27.69970515165579
45	20.716508777198396	21.08250976002621	29.096310300581507	29.104671162193892
46	21.157074995222363	20.88014278304076	31.521983947145703	26.44079827459117
47	17.90913620355456	23.58053045401185	33.744608097409156	24.765725245024434
48	18.826612793142044	23.360247344999863	32.34237216413224	25.470767697725844
49	22.350289388189687	19.25062791777007	33.24227388135084	25.156808812689402
50	19.507596439979253	22.199452619509135	31.184136886073876	27.10881405443774
51	18.528522482186247	21.7365338939092	28.665128723142864	31.069814900761695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	334.5
2	591.0
3	2057.0
4	3523.0
5	2275.0
6	1027.0
7	1066.0
8	1105.0
9	1229.5
10	1354.0
11	1301.5
12	1249.0
13	1203.5
14	1158.0
15	1144.5
16	1131.0
17	1188.5
18	1246.0
19	1315.0
20	1384.0
21	1470.5
22	1557.0
23	1966.0
24	2375.0
25	3026.5
26	4205.5
27	4733.0
28	5116.0
29	5499.0
30	6735.5
31	7972.0
32	9297.0
33	10622.0
34	12026.0
35	13430.0
36	14859.5
37	16289.0
38	18104.5
39	19920.0
40	23719.5
41	27519.0
42	31070.5
43	34622.0
44	37995.5
45	41369.0
46	47138.0
47	52907.0
48	59835.5
49	66764.0
50	64666.0
51	62568.0
52	54794.5
53	47021.0
54	42918.0
55	38815.0
56	34523.0
57	30231.0
58	27363.5
59	24496.0
60	21640.5
61	18785.0
62	16212.0
63	13639.0
64	11476.5
65	9314.0
66	7971.5
67	6629.0
68	5623.5
69	4618.0
70	3742.0
71	2866.0
72	2349.5
73	1833.0
74	1442.0
75	793.0
76	535.0
77	439.0
78	343.0
79	215.5
80	88.0
81	53.0
82	18.0
83	42.5
84	67.0
85	48.5
86	30.0
87	19.5
88	9.0
89	5.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	586064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.59949864555824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28450479066292	23.615241235176107
2	11.689308064625225	7.855097809697767
3	4.4506983188022495	4.486236964031536
4	2.3385793773510857	3.1430037848735304
5	1.4770547398677762	2.4814149365801375
6	1.1160745933721463	2.249972807300964
7	0.8691159193197464	2.0441301407811854
8	0.7389098339027802	1.9861599970724908
9	0.6640831789077303	2.0081575683223467
>10	6.181307768519197	42.08773480719149
>50	0.14303549389508455	3.176678366269979
>100	0.04154339712391963	2.613042792076787
>500	0.003155194718272377	0.6449121773669803
>1k	0.0026293289318936475	1.6082166132587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	2123	0.3622471265936826	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2114	0.3607114581342652	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1730	0.2951896038657894	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1587	0.27078953834393515	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1548	0.26413497501979305	No Hit
GCTGTATCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	680	0.1160282836004259	TruSeq Adapter, Index 27 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	627	0.10698490267274563	TruSeq Adapter, Index 27 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	593	0.10118348849272435	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	591	0.10084222883507603	TruSeq Adapter, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.382381446394933	0.0
2	0.0	0.0	0.0	1.3786890168991783	0.0
3	0.0	0.0	0.0	1.9477394960277377	0.0
4	0.0	0.0	0.0	2.52634524557045	0.0
5	0.0	0.0	0.0	3.485284883562205	0.0
6	0.0	0.0	0.0	4.096480930410331	0.0
7	0.0	0.0	0.0	4.877965546424964	0.0
8	0.0	0.0	0.0	6.077493243058779	0.0
9	0.0	0.0	0.0	6.625385623413143	0.0
10	0.0	0.0	0.0	7.67885418657348	0.0
11	0.0	0.0	0.0	9.846876791613203	0.0
12	0.0	0.0	0.0	11.608288514564963	0.0
13	0.0	0.0	0.0	12.20020339075596	0.0
14	0.0	0.0	0.0	12.423557836686777	0.0
15	0.0	0.0	0.0	12.767035682109803	0.0
16	0.0	0.0	0.0	13.536576210106746	0.0
17	0.0	0.0	0.0	14.569569193808185	0.0
18	0.0	0.0	0.0	15.720467389227116	0.0
19	0.0	0.0	0.0	16.477551939719895	0.0
20	0.0	0.0	0.0	17.32967730486773	0.0
21	0.0	0.0	0.0	18.494737776079063	0.0
22	0.0	0.0	0.0	19.61679953042671	0.0
23	0.0	0.0	0.0	20.621126702885693	0.0
24	0.0	0.0	0.0	21.39698053454913	0.0
25	0.0	0.0	0.0	22.10492369434055	0.0
26	0.0	0.0	0.0	22.776522700592427	0.0
27	0.0	0.0	0.0	23.476787518086763	0.0
28	0.0	0.0	0.0	24.135930546834476	0.0
29	0.0	0.0	0.0	24.884824865543695	0.0
30	0.0	0.0	0.0	25.809467907941794	0.0
31	0.0	0.0	0.0	26.4650277102842	0.0
32	0.0	0.0	0.0	27.136114827049607	0.0
33	0.0	0.0	0.0	27.777853613257257	0.0
34	0.0	0.0	0.0	28.485455513391027	0.0
35	0.0	0.0	0.0	29.28451500177455	0.0
36	1.7062982882415573E-4	0.0	0.0	29.931713942504572	0.0
37	1.7062982882415573E-4	0.0	0.0	30.636415135548337	0.0
38	1.7062982882415573E-4	0.0	0.0	31.43274454667067	0.0
39	1.7062982882415573E-4	0.0	0.0	32.763315951841435	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAA	20	7.030454E-4	45.0	17
TCCGCAC	20	7.030454E-4	45.0	31
TTAATCG	25	3.888458E-5	45.0	20
ACGTTAC	20	7.030454E-4	45.0	12
CGTGCGA	20	7.030454E-4	45.0	31
CCCTACT	20	7.030454E-4	45.0	23
ATAGCGT	20	7.030454E-4	45.0	17
CGTTACG	20	7.030454E-4	45.0	13
GCCGATT	55	1.8189894E-12	45.0	9
AATTGCG	20	7.030454E-4	45.0	1
GTCGTTG	20	7.030454E-4	45.0	1
TTGCACG	20	7.030454E-4	45.0	2
TGTGTCG	40	6.8048394E-9	45.0	1
CGATAGC	20	7.030454E-4	45.0	15
ACCTTCG	20	7.030454E-4	45.0	23
GTAGTAC	40	6.8048394E-9	45.0	9
ACGGTGC	20	7.030454E-4	45.0	41
ACGAACA	30	2.1636752E-6	44.999996	25
CGTTAGG	145	0.0	43.448273	2
CTAGGGA	655	0.0	42.938927	4
>>END_MODULE