FastQCFastQC Report
Sat 14 Jan 2017
SRR2934237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378975
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT28570.20718287133559346TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC25500.1849199586649504TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC21110.15308471872223936TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC21020.15243206004459836TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.11639079751264528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAC207.0337765E-445.00000433
GCGATAT453.8562575E-1045.09
TCGATAG253.8912138E-544.9999961
CCGTTAG600.044.9999961
CGTTAGG3700.043.1756742
TAGGGCG7300.042.534255
CTCGTAG1250.041.41
TAGGGCA12900.041.1627925
GACACGA4550.041.04395725
TCGTTAG2200.040.9090921
CTAGGGA16150.040.6811144
TAGGGAG16050.040.514025
CGTAAGG501.0822987E-940.4999962
TTTAGGG57300.040.484293
TTAGGGA29500.040.423734
AGACACG4750.040.26315724
CACGACC4650.040.16129327
AGGGCGA8350.040.14976
TGTAGGG21300.039.8239443
CTTAGGG25900.039.61393
Produced by FastQC (version 0.11.5)