##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1378975
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41510614768215	33.0	31.0	34.0	31.0	34.0
2	32.58058848057434	34.0	31.0	34.0	31.0	34.0
3	32.650209032070926	34.0	31.0	34.0	31.0	34.0
4	36.08931779038779	37.0	35.0	37.0	35.0	37.0
5	35.16979785710401	37.0	35.0	37.0	35.0	37.0
6	25.923911601007994	37.0	0.0	37.0	0.0	37.0
7	31.04138291122029	37.0	19.0	37.0	17.0	37.0
8	34.81730923330735	37.0	32.0	37.0	32.0	37.0
9	37.39575844377164	39.0	37.0	39.0	35.0	39.0
10	37.36081074711289	39.0	37.0	39.0	34.0	39.0
11	37.44997407494697	39.0	37.0	39.0	35.0	39.0
12	37.101875668521906	39.0	37.0	39.0	34.0	39.0
13	37.04865715477075	39.0	37.0	39.0	33.0	39.0
14	37.917061585598	40.0	37.0	41.0	33.0	41.0
15	38.144272376221465	40.0	37.0	41.0	33.0	41.0
16	38.31887162566399	40.0	37.0	41.0	33.0	41.0
17	38.36206385177396	40.0	37.0	41.0	34.0	41.0
18	38.36321688210446	40.0	37.0	41.0	34.0	41.0
19	38.37836073895466	40.0	37.0	41.0	34.0	41.0
20	38.27700429666963	40.0	37.0	41.0	34.0	41.0
21	38.18656320817999	40.0	37.0	41.0	34.0	41.0
22	38.24300585579869	40.0	37.0	41.0	34.0	41.0
23	38.19954313892565	40.0	36.0	41.0	34.0	41.0
24	38.145629181094655	40.0	36.0	41.0	34.0	41.0
25	38.04363240812923	40.0	36.0	41.0	34.0	41.0
26	38.013311336318644	40.0	36.0	41.0	34.0	41.0
27	38.00054170670244	40.0	36.0	41.0	34.0	41.0
28	37.94056890081401	40.0	36.0	41.0	34.0	41.0
29	37.93182690041516	40.0	36.0	41.0	34.0	41.0
30	37.69347812686959	40.0	36.0	41.0	33.0	41.0
31	37.67178012654327	40.0	36.0	41.0	33.0	41.0
32	37.645356152214504	40.0	36.0	41.0	33.0	41.0
33	37.595825159992025	40.0	36.0	41.0	33.0	41.0
34	37.50294530357693	40.0	36.0	41.0	33.0	41.0
35	37.48606392429159	40.0	35.0	41.0	33.0	41.0
36	37.42899472434235	40.0	35.0	41.0	33.0	41.0
37	37.342141808227126	40.0	35.0	41.0	32.0	41.0
38	37.28283471418989	40.0	35.0	41.0	32.0	41.0
39	37.25345999746188	40.0	35.0	41.0	32.0	41.0
40	37.15120578690694	40.0	35.0	41.0	32.0	41.0
41	37.016027121593936	39.0	35.0	41.0	32.0	41.0
42	37.02350006345293	39.0	35.0	41.0	32.0	41.0
43	37.01031128193042	39.0	35.0	41.0	32.0	41.0
44	36.92491814572418	39.0	35.0	41.0	32.0	41.0
45	36.89779800213927	39.0	35.0	41.0	32.0	41.0
46	36.849771025580594	39.0	35.0	41.0	32.0	41.0
47	36.7697804528726	39.0	35.0	41.0	32.0	41.0
48	36.730684022552985	39.0	35.0	41.0	31.0	41.0
49	36.718696858173644	39.0	35.0	41.0	31.0	41.0
50	36.625733606483074	39.0	35.0	41.0	31.0	41.0
51	35.649382331079245	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	7.0
10	8.0
11	14.0
12	7.0
13	13.0
14	22.0
15	37.0
16	80.0
17	162.0
18	252.0
19	496.0
20	829.0
21	1399.0
22	2195.0
23	3201.0
24	4823.0
25	6851.0
26	8765.0
27	10111.0
28	11842.0
29	14619.0
30	19172.0
31	26208.0
32	38452.0
33	63721.0
34	93103.0
35	100962.0
36	115847.0
37	204435.0
38	302747.0
39	348370.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.756793995540168	4.220671150673508	55.834224695879186	15.188310157907143
2	19.84764045758625	4.297612357004297	54.58133758770101	21.273409597708444
3	21.683714353052085	4.1716492322195835	52.69609673851955	21.448539676208778
4	19.24146558131946	4.558603310429849	52.0504722710709	24.14945883717979
5	17.94869377617433	7.964756431407386	51.03515292155405	23.05139687086423
6	16.150546601642525	31.52051342482641	39.28838448847876	13.040555485052302
7	85.84136768251781	1.6875577874870828	8.31472651788466	4.156348012110445
8	89.1211950905564	1.8800195797603292	5.625265142587792	3.3735201870954876
9	85.05708950488588	3.542051161188564	7.524864482677351	3.875994851248209
10	40.89392483547562	23.21528671658297	19.27395347993981	16.616834968001594
11	33.74158342246959	26.287278594608317	22.195616309215175	17.77552167370692
12	29.809097336790007	22.669809097336792	27.672655414347613	19.848438151525592
13	28.060987327544005	22.12077811417901	29.123950760528654	20.694283797748326
14	22.53260573977048	23.375913268913504	32.738011929150275	21.35346906216574
15	22.925578781341212	23.231240595369748	33.20372015446255	20.639460468826485
16	26.21425334034337	23.62653420112765	29.10045504813358	21.0587574103954
17	26.959009409162604	23.682155223988833	27.60978262840153	21.749052738447038
18	27.13986838050001	23.1405935568085	29.02336880654109	20.6961692561504
19	26.83725230696713	25.349553110099894	27.370111858445583	20.44308272448739
20	25.70365670153556	29.178991642343043	27.293823310792437	17.82352834532896
21	24.92032125310466	26.276908573396906	30.36726554143476	18.43550463206367
22	23.256476730905202	22.960822349933828	31.79274461103356	21.989956308127415
23	21.910839572871154	25.52896172882032	30.667125944995377	21.89307275331315
24	22.073279065972915	27.41478271904857	29.033593792490798	21.478344422487716
25	23.94437897713882	26.25689370728258	26.62281767254664	23.17590964303196
26	21.121775231603184	26.26726372849399	27.14102866259359	25.469932377309235
27	20.628582824199132	25.473413223589986	30.919632335611595	22.978371616599286
28	18.45073333454196	25.799960115303033	30.212802987726388	25.536503562428614
29	20.610163345963485	26.318968799289326	29.611051686941387	23.459816167805798
30	24.348012110444355	24.25453688427999	29.911709784441342	21.485741220834313
31	23.158287858735655	25.77617433238456	29.768777534037966	21.296760274841823
32	24.447071194184087	25.799815080041334	27.598034772203995	22.155078953570587
33	24.729962472126036	24.806105984517483	27.55329139397016	22.910640149386317
34	20.835910730796424	24.475643140738594	31.016878478580107	23.671567649884878
35	20.330607879040592	27.008974056817564	28.212694211280116	24.447723852861728
36	23.37344766946464	25.97342228829384	27.4522743341975	23.200855708044017
37	20.71444369912435	27.05081672981744	30.09104588553092	22.143693685527293
38	22.32875867945394	25.973132217770445	29.163545386972206	22.534563715803404
39	22.435504632063672	24.158596058666763	28.052865352888922	25.353033956380642
40	25.62845591834515	23.539005420692906	28.07563588897551	22.75690277198644
41	21.54549574865389	24.761145053391107	26.881850649939267	26.811508548015738
42	20.0543157055059	24.87753585090375	29.788937435413988	25.27921100817636
43	20.57281676607625	23.241610616581156	30.661832157943397	25.523740459399193
44	22.314617741438386	23.895212023423195	29.112202904331113	24.677967330807302
45	21.735999564894215	22.644355408908794	27.508693050998023	28.110951975198972
46	21.462680614224332	23.31927699922044	29.384289055276565	25.83375333127867
47	18.938051813847242	25.025979441251657	31.976866875759168	24.059101869141937
48	20.24083105204953	26.485034173933535	30.288656429594447	22.98547834442249
49	23.267354375532552	22.71179680559836	30.36596022407948	23.654888594789607
50	21.725919614206205	24.033285592559693	28.17861092478109	26.062183868453015
51	20.419514494461467	24.74577131565112	26.848855127902972	27.985859061984446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	807.0
2	1409.0
3	2761.0
4	4113.0
5	2944.0
6	1775.0
7	1823.0
8	1871.0
9	1966.0
10	2061.0
11	2172.5
12	2284.0
13	2256.5
14	2229.0
15	2188.5
16	2148.0
17	2147.0
18	2146.0
19	2300.5
20	2455.0
21	2832.5
22	3210.0
23	3775.5
24	4341.0
25	5333.0
26	7316.5
27	8308.0
28	10037.0
29	11766.0
30	15156.5
31	18547.0
32	21296.5
33	24046.0
34	29391.5
35	34737.0
36	38436.0
37	42135.0
38	47752.0
39	53369.0
40	59206.0
41	65043.0
42	73574.5
43	82106.0
44	90567.5
45	99029.0
46	111767.5
47	124506.0
48	139305.5
49	154105.0
50	152035.0
51	149965.0
52	135130.0
53	120295.0
54	109312.0
55	98329.0
56	88639.0
57	78949.0
58	69641.0
59	60333.0
60	50632.5
61	40932.0
62	34535.0
63	28138.0
64	23393.0
65	18648.0
66	15079.5
67	11511.0
68	9396.0
69	7281.0
70	5734.5
71	4188.0
72	3426.0
73	2664.0
74	2247.5
75	1405.5
76	980.0
77	669.0
78	358.0
79	263.0
80	168.0
81	124.0
82	80.0
83	56.5
84	33.0
85	25.5
86	18.0
87	11.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1378975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.05980892118213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.29672618781218	21.834028831781065
2	11.320634388235783	7.03233481930334
3	4.101444670244704	3.8217026327580417
4	2.2057086173560725	2.740355527635378
5	1.4119289154400008	2.1927121161929177
6	1.0435124663649196	1.9446778687299542
7	0.8565353540266449	1.8622677101213279
8	0.7130413399152626	1.7717542216537387
9	0.6260296771411613	1.7499925935894434
>10	7.184801481392624	46.936573555923026
>50	0.17985975607515073	3.5899534779208144
>100	0.05487738012424455	2.866495558335812
>500	0.00342983610991827	0.7199909806342957
>1k	0.0014699297613935446	0.9371601054208397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	2857	0.20718287133559346	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	2550	0.1849199586649504	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	2111	0.15308471872223936	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	2102	0.15243206004459836	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1605	0.11639079751264528	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4503526169800033E-4	0.0	0.0	0.2182780688554905	0.0
2	1.4503526169800033E-4	0.0	0.0	0.7497597853478127	0.0
3	1.4503526169800033E-4	0.0	0.0	1.0556391522688953	0.0
4	1.4503526169800033E-4	0.0	0.0	1.3953117351656121	0.0
5	1.4503526169800033E-4	0.0	0.0	1.9824869921499664	0.0
6	1.4503526169800033E-4	0.0	0.0	2.3982305698072843	0.0
7	1.4503526169800033E-4	0.0	0.0	2.9534980692180786	0.0
8	1.4503526169800033E-4	0.0	0.0	3.8513388567595497	0.0
9	1.4503526169800033E-4	0.0	0.0	4.243006580975	0.0
10	1.4503526169800033E-4	0.0	0.0	5.087836980365852	0.0
11	1.4503526169800033E-4	0.0	0.0	6.632680070342102	0.0
12	1.4503526169800033E-4	0.0	0.0	7.930455592015809	0.0
13	1.4503526169800033E-4	0.0	0.0	8.36773690603528	0.0
14	2.1755289254700048E-4	0.0	0.0	8.532859551478452	0.0
15	2.1755289254700048E-4	0.0	0.0	8.749107126670172	0.0
16	2.1755289254700048E-4	0.0	0.0	9.283634583658152	0.0
17	2.1755289254700048E-4	0.0	0.0	10.02142895991588	0.0
18	2.9007052339600066E-4	0.0	0.0	10.82789753258761	0.0
19	2.9007052339600066E-4	0.0	0.0	11.416668177450642	0.0
20	2.9007052339600066E-4	0.0	0.0	12.072662666110698	0.0
21	2.9007052339600066E-4	0.0	0.0	13.008430174586197	0.0
22	3.6258815424500084E-4	0.0	0.0	14.03890570895049	0.0
23	4.3510578509400096E-4	0.0	0.0	14.982070015772585	0.0
24	4.3510578509400096E-4	0.0	0.0	15.71935676861437	0.0
25	4.3510578509400096E-4	0.0	0.0	16.3773817509382	0.0
26	4.3510578509400096E-4	0.0	0.0	16.963396725828968	0.0
27	5.801410467920013E-4	0.0	0.0	17.63541761090665	0.0
28	5.801410467920013E-4	0.0	0.0	18.24703130948712	0.0
29	5.801410467920013E-4	0.0	0.0	18.987726390978807	0.0
30	5.801410467920013E-4	0.0	0.0	19.817618158414763	0.0
31	6.526586776410014E-4	0.0	0.0	20.459544226690113	0.0
32	6.526586776410014E-4	0.0	0.0	21.075944088906617	0.0
33	6.526586776410014E-4	0.0	0.0	21.69662249134321	0.0
34	6.526586776410014E-4	0.0	0.0	22.377055421599376	0.0
35	6.526586776410014E-4	0.0	0.0	23.12826555956417	0.0
36	7.251763084900017E-4	0.0	0.0	23.75264236117406	0.0
37	7.251763084900017E-4	0.0	0.0	24.43002955093457	0.0
38	7.251763084900017E-4	0.0	0.0	25.26492503489911	0.0
39	7.251763084900017E-4	0.0	0.0	26.81651226454432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCAC	20	7.0337765E-4	45.000004	33
GCGATAT	45	3.8562575E-10	45.0	9
TCGATAG	25	3.8912138E-5	44.999996	1
CCGTTAG	60	0.0	44.999996	1
CGTTAGG	370	0.0	43.175674	2
TAGGGCG	730	0.0	42.53425	5
CTCGTAG	125	0.0	41.4	1
TAGGGCA	1290	0.0	41.162792	5
GACACGA	455	0.0	41.043957	25
TCGTTAG	220	0.0	40.909092	1
CTAGGGA	1615	0.0	40.681114	4
TAGGGAG	1605	0.0	40.51402	5
CGTAAGG	50	1.0822987E-9	40.499996	2
TTTAGGG	5730	0.0	40.48429	3
TTAGGGA	2950	0.0	40.42373	4
AGACACG	475	0.0	40.263157	24
CACGACC	465	0.0	40.161293	27
AGGGCGA	835	0.0	40.1497	6
TGTAGGG	2130	0.0	39.823944	3
CTTAGGG	2590	0.0	39.6139	3
>>END_MODULE