##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934236.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	604704
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.280274316029	33.0	31.0	34.0	30.0	34.0
2	32.456620098428324	34.0	31.0	34.0	31.0	34.0
3	32.50325448483886	34.0	31.0	34.0	30.0	34.0
4	36.00472958670689	37.0	35.0	37.0	35.0	37.0
5	35.09273131978621	37.0	35.0	37.0	35.0	37.0
6	25.792928441022383	36.0	0.0	37.0	0.0	37.0
7	30.93053130126475	37.0	19.0	37.0	17.0	37.0
8	34.636526300470976	37.0	32.0	37.0	32.0	37.0
9	37.18370144731968	39.0	37.0	39.0	34.0	39.0
10	37.321004987564166	39.0	37.0	39.0	34.0	39.0
11	37.385492736942375	39.0	37.0	39.0	35.0	39.0
12	37.017486241202306	39.0	35.0	39.0	34.0	39.0
13	36.934301741017094	39.0	35.0	39.0	33.0	39.0
14	37.88916230089432	40.0	37.0	41.0	33.0	41.0
15	38.07617611261047	40.0	37.0	41.0	33.0	41.0
16	38.266649468169554	40.0	37.0	41.0	34.0	41.0
17	38.273338691326664	40.0	37.0	41.0	34.0	41.0
18	38.25676529343282	40.0	37.0	41.0	34.0	41.0
19	38.256766947134466	40.0	37.0	41.0	34.0	41.0
20	38.15955409588824	40.0	36.0	41.0	34.0	41.0
21	38.07122162247976	40.0	36.0	41.0	34.0	41.0
22	38.094566928613006	40.0	36.0	41.0	34.0	41.0
23	38.050684301741015	40.0	36.0	41.0	34.0	41.0
24	37.985174564745726	40.0	36.0	41.0	34.0	41.0
25	37.86960893263481	40.0	36.0	41.0	34.0	41.0
26	37.84353501878605	40.0	36.0	41.0	34.0	41.0
27	37.82415198179605	40.0	36.0	41.0	34.0	41.0
28	37.783933957771076	40.0	36.0	41.0	34.0	41.0
29	37.76950044980685	40.0	36.0	41.0	34.0	41.0
30	37.52317662856538	40.0	35.0	41.0	33.0	41.0
31	37.48570044186908	39.0	35.0	41.0	33.0	41.0
32	37.42929102503043	39.0	35.0	41.0	33.0	41.0
33	37.33855572313065	39.0	35.0	41.0	33.0	41.0
34	37.217160792718424	39.0	35.0	41.0	32.0	41.0
35	37.21148859607345	39.0	35.0	41.0	32.0	41.0
36	37.14338254749431	39.0	35.0	41.0	32.0	41.0
37	37.03895624966926	39.0	35.0	41.0	32.0	41.0
38	36.96852013547124	39.0	35.0	41.0	32.0	41.0
39	36.91422745673916	39.0	35.0	41.0	32.0	41.0
40	36.77594823252368	39.0	35.0	41.0	31.0	41.0
41	36.65038266656083	39.0	35.0	41.0	31.0	41.0
42	36.70409654971689	39.0	35.0	41.0	31.0	41.0
43	36.65639717944647	39.0	35.0	41.0	31.0	41.0
44	36.53263084087421	39.0	35.0	41.0	31.0	41.0
45	36.49801886542838	39.0	35.0	40.0	31.0	41.0
46	36.41725207704927	39.0	35.0	40.0	31.0	41.0
47	36.336529938614596	38.0	35.0	40.0	31.0	41.0
48	36.33913286500503	38.0	35.0	40.0	31.0	41.0
49	36.33895426522729	38.0	35.0	40.0	31.0	41.0
50	36.2352638646346	38.0	35.0	40.0	31.0	41.0
51	35.197276684129754	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	11.0
10	11.0
11	5.0
12	11.0
13	2.0
14	7.0
15	16.0
16	25.0
17	44.0
18	105.0
19	190.0
20	346.0
21	551.0
22	898.0
23	1307.0
24	2108.0
25	3184.0
26	4359.0
27	5271.0
28	5983.0
29	7110.0
30	9234.0
31	12263.0
32	17606.0
33	29283.0
34	43341.0
35	52173.0
36	57486.0
37	94492.0
38	127650.0
39	129563.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.317014605492936	4.723964121289093	52.48121394930412	17.47780732391385
2	21.33886992644335	5.832274964280044	51.148661163147594	21.680193946129016
3	22.022344816637563	5.855592157485315	50.11046726993703	22.011595755940096
4	19.137131555273324	4.347581626713235	51.89828411917235	24.617002698841087
5	19.24214160977933	8.0583227496428	48.71970418584961	23.979831454728266
6	15.952267555696672	32.36327194792824	38.514049849182406	13.170410647192677
7	80.6343268772821	2.41539662380272	11.927488490236545	5.022788008678626
8	80.91049505212467	3.55397020691115	9.848124040853046	5.687410700111128
9	75.50371752129968	4.775228872307774	12.019434301741017	7.7016193046515316
10	41.205780017992275	23.390286818013443	19.563455839551253	15.840477324443034
11	31.871626448642644	22.452472614700746	25.928222733767264	19.74767820288935
12	28.925722336878867	19.978534952637986	30.86915251098058	20.226590199502567
13	25.590371487537706	22.607755199237975	32.297619992591414	19.504253320632905
14	20.33126951367942	25.989740434989685	32.68326321638355	20.995726834947344
15	16.667162777160396	23.856630682118855	37.91408689209928	21.562119648621476
16	21.920642165423082	23.3198391279039	32.24784357305393	22.511675133619093
17	22.4976186696301	22.89169577181563	29.84815711488596	24.76252844366831
18	22.859613959887813	22.701520082552786	32.44959517383712	21.989270783722283
19	23.700190506429593	24.190843784727733	30.123002328411918	21.985963380430757
20	24.117750171984973	26.86289490395301	29.605062972958674	19.414291951103348
21	23.16736783616447	25.673883420648778	31.47440731333016	19.68434142985659
22	21.205912314123935	22.72748319839128	31.981432237921364	24.08517224956342
23	21.5353296819601	24.644454146160765	30.17592078107636	23.64429539080277
24	21.93635233105784	23.68563793194687	29.99517119119437	24.38283854580092
25	21.74733423294703	25.062013811716145	27.757878234640422	25.432773720696407
26	18.800272530031222	25.348104196433297	29.3821439911097	26.469479282425784
27	19.678718844260995	24.74218791342541	30.70923956183521	24.869853680478382
28	16.90050007937768	23.361843149706303	32.51855453246547	27.21910223845055
29	19.331441498650577	22.769156479864527	32.341277716039585	25.558124305445308
30	22.21516642853363	23.111803460866803	30.7520704344605	23.92095967613907
31	21.9016245964968	25.446664814520826	28.011556067100596	24.64015452188178
32	23.121891040905965	24.42550404826163	27.718850875800392	24.733754035032014
33	22.974876964597556	23.071122400381014	28.160389215219343	25.793611419802087
34	19.319700216965654	23.224916653437052	31.347733767264646	26.107649362332648
35	18.390981372704662	23.645783722283962	30.33070725511986	27.632527649891518
36	22.40782267026512	23.083690532888816	29.668565116156003	24.839921680690054
37	19.218493676244908	24.658841350478912	30.281096205746945	25.84156876752924
38	20.408001270042863	24.017370482087102	28.550828173784197	27.023800074085834
39	21.91121606604223	22.96958511933111	30.959940731333013	24.159258083293643
40	23.888051013388367	22.660342911573267	29.511794200137587	23.939811874900776
41	18.847899137429224	23.522913690003705	28.274990739270784	29.35419643329629
42	19.709312324707625	21.10685558554268	31.291342541144097	27.8924895486056
43	20.894685664391176	20.632573953537598	31.504339313118486	26.968401068952748
44	20.72633883685241	21.50738212414669	29.94208736836535	27.82419167063555
45	20.9008043604805	20.920814150394243	28.235467269937025	29.942914219188232
46	21.840933746097264	22.186557390061914	30.113576229031064	25.858932634809754
47	17.141775149494627	23.190354289040588	33.5618749007779	26.105995660686883
48	18.524931206011537	22.73707466793671	31.371712441128217	27.366281684923532
49	21.15679737524475	20.11331163676774	33.708558236757156	25.021332751230354
50	21.11495872360692	20.79463671482246	29.97664973276181	28.11375482880881
51	19.221801079536434	21.88227628724136	27.325765994602317	31.57015663861989
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	362.5
2	600.0
3	2774.5
4	4949.0
5	3213.5
6	1478.0
7	1432.0
8	1386.0
9	1465.0
10	1544.0
11	1573.0
12	1602.0
13	1580.5
14	1559.0
15	1458.0
16	1357.0
17	1279.5
18	1202.0
19	1201.5
20	1201.0
21	1318.5
22	1436.0
23	1879.5
24	2323.0
25	2615.0
26	3531.0
27	4155.0
28	4968.0
29	5781.0
30	6421.5
31	7062.0
32	8211.5
33	9361.0
34	10921.5
35	12482.0
36	13380.0
37	14278.0
38	16445.5
39	18613.0
40	22101.5
41	25590.0
42	29114.5
43	32639.0
44	37056.0
45	41473.0
46	52844.0
47	64215.0
48	69406.0
49	74597.0
50	71500.5
51	68404.0
52	58344.0
53	48284.0
54	42425.0
55	36566.0
56	33611.5
57	30657.0
58	27650.0
59	24643.0
60	21784.5
61	18926.0
62	16638.5
63	14351.0
64	12062.0
65	9773.0
66	8088.5
67	6404.0
68	5658.5
69	4913.0
70	3997.0
71	3081.0
72	2623.0
73	2165.0
74	1719.0
75	970.0
76	667.0
77	523.5
78	380.0
79	291.0
80	202.0
81	132.5
82	63.0
83	42.5
84	22.0
85	16.5
86	11.0
87	6.0
88	1.0
89	0.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	604704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.32397479753425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79512186441839	23.956460038651283
2	12.134143226597534	8.329840525988095
3	4.630487315445504	4.768101897469606
4	2.4554949695828556	3.371293898057362
5	1.5839233500751904	2.7183272574603423
6	1.1108697005708488	2.287767816344294
7	0.8945948715586918	2.149423627776875
8	0.7715973911824658	2.118743152703211
9	0.6408987185418072	1.979837231670089
>10	5.811542252455459	38.88919076173999
>50	0.11853392098270359	2.7525999035966575
>100	0.04432570994704472	2.7568735961430533
>500	0.004482375163184298	1.0397039258226608
>1k	0.003984333478386042	2.8818363665765236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2439	0.4033378314018098	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2319	0.3834934116526433	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2269	0.3752249034238239	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	2268	0.3750595332592475	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	2264	0.37439805260094194	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	1963	0.3246216330634492	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1917	0.3170146054929354	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	1419	0.23466026353389427	No Hit
GCTGTATCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	874	0.14453352383976292	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.13907630840874213	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	726	0.12005873948245753	No Hit
TCTGTATCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	699	0.11559374503889507	No Hit
CCTGTATCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	676	0.11179023125363814	No Hit
CTGTCACTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	661	0.10930967878499233	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6537016457638778E-4	0.0	0.0	0.39523469333756683	0.0
2	1.6537016457638778E-4	0.0	0.0	1.589372651743663	0.0
3	1.6537016457638778E-4	0.0	0.0	2.225055564375298	0.0
4	1.6537016457638778E-4	0.0	0.0	2.91944488543155	0.0
5	1.6537016457638778E-4	0.0	0.0	4.221073450812298	0.0
6	1.6537016457638778E-4	0.0	0.0	4.875773932370217	0.0
7	1.6537016457638778E-4	0.0	0.0	5.849969571889718	0.0
8	1.6537016457638778E-4	0.0	0.0	7.23659840186273	0.0
9	1.6537016457638778E-4	0.0	0.0	7.866327988569614	0.0
10	1.6537016457638778E-4	0.0	0.0	9.380622585595598	0.0
11	1.6537016457638778E-4	0.0	0.0	11.70109673493147	0.0
12	1.6537016457638778E-4	0.0	0.0	14.142456474572683	0.0
13	3.3074032915277556E-4	0.0	0.0	14.854209662909456	0.0
14	3.3074032915277556E-4	0.0	0.0	15.095980843520135	0.0
15	3.3074032915277556E-4	0.0	0.0	15.548929724294862	0.0
16	3.3074032915277556E-4	0.0	0.0	16.40554717680055	0.0
17	4.961104937291634E-4	0.0	0.0	17.551727787479493	0.0
18	4.961104937291634E-4	0.0	0.0	18.836323225908874	0.0
19	4.961104937291634E-4	0.0	0.0	19.74073265597714	0.0
20	4.961104937291634E-4	0.0	0.0	20.683342594062548	0.0
21	4.961104937291634E-4	0.0	0.0	21.907247182092394	0.0
22	4.961104937291634E-4	0.0	0.0	23.207056675662805	0.0
23	4.961104937291634E-4	0.0	0.0	24.32198232523681	0.0
24	4.961104937291634E-4	0.0	0.0	25.19364846271895	0.0
25	4.961104937291634E-4	0.0	0.0	25.956501031909827	0.0
26	4.961104937291634E-4	0.0	0.0	26.668088850082025	0.0
27	4.961104937291634E-4	0.0	0.0	27.46170026988411	0.0
28	4.961104937291634E-4	0.0	0.0	28.1579086627507	0.0
29	4.961104937291634E-4	0.0	0.0	28.967726358681272	0.0
30	4.961104937291634E-4	0.0	0.0	29.869159125787164	0.0
31	4.961104937291634E-4	0.0	0.0	30.623247076255492	0.0
32	4.961104937291634E-4	0.0	0.0	31.304241413981057	0.0
33	4.961104937291634E-4	0.0	0.0	32.01252182886172	0.0
34	4.961104937291634E-4	0.0	0.0	32.789100121712444	0.0
35	4.961104937291634E-4	0.0	0.0	33.60999761866963	0.0
36	4.961104937291634E-4	0.0	0.0	34.33101153622268	0.0
37	4.961104937291634E-4	0.0	0.0	35.04111102291369	0.0
38	4.961104937291634E-4	0.0	0.0	35.83951817748849	0.0
39	4.961104937291634E-4	0.0	0.0	37.078140710165634	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAGG	85	0.0	45.000004	2
ACGGATC	30	2.163788E-6	45.000004	14
CGGGTAT	40	6.8066583E-9	45.0	6
GACGTAT	20	7.0306315E-4	45.0	16
CCGTAGG	20	7.0306315E-4	45.0	2
CGATGCG	20	7.0306315E-4	45.0	10
GGCGATA	20	7.0306315E-4	45.0	8
ATCGGAC	20	7.0306315E-4	45.0	42
CGGACTT	20	7.0306315E-4	45.0	15
CGGACTC	20	7.0306315E-4	45.0	44
TTTACGC	25	3.8886064E-5	44.999996	23
CGTTAGG	200	0.0	44.999996	2
CCCGTTA	25	3.8886064E-5	44.999996	43
ATCGGCA	25	3.8886064E-5	44.999996	24
ACTCAAC	25	3.8886064E-5	44.999996	29
TAGGGCG	255	0.0	43.235294	5
ACATACG	115	0.0	43.043476	17
TCGTTAG	95	0.0	42.63158	1
TTTGTCG	95	0.0	42.63158	1
GCGTTAG	75	0.0	42.0	1
>>END_MODULE