FastQCFastQC Report
Sat 14 Jan 2017
SRR2934235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137139
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT9240.6737689497517118TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC8960.6533517088501447TruSeq Adapter, Index 22 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC7510.5476195684670299TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC5820.42438693588257176TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG2640.1925054142147748No Hit
CCTGTATCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC2620.19104703986466284TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT2610.1903178526896069TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG2480.18083841941387935No Hit
TCTGTATCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC2400.17500492201343162TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC2360.17208817331320778No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG2080.15167093241164076No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.12687856845973794No Hit
GCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG1730.12614938128468195No Hit
GCTGTATCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC1690.1232326325844581TruSeq Adapter, Index 20 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT1690.1232326325844581No Hit
TTCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT1650.12031588388423424No Hit
TGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG1580.11521157365884248No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG1440.10500295320805897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGT302.1514898E-645.00000430
AGGGCAC302.1514898E-645.0000046
CTGTCGG207.0115447E-445.02
TCGTTAG253.8727943E-545.01
ACACGCG453.8016879E-1045.036
CTCGTAA351.201497E-745.033
CGCGGTA207.0115447E-445.030
AGATAAA351.201497E-745.018
TTGCTAG351.201497E-745.01
TGGGTCC253.8727943E-545.06
GACAAAC207.0115447E-445.012
ACTCTAC207.0115447E-445.011
CACGCGG502.1827873E-1145.037
GTCTAGG207.0115447E-445.02
TAAAACG351.201497E-745.021
ACTTAGG207.0115447E-445.02
GCGTTAG453.8016879E-1045.01
AAAACGA351.201497E-745.022
GCACCGA351.201497E-745.09
CGTTAGG750.045.02
Produced by FastQC (version 0.11.5)