##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934235.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	137139
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26004273036846	33.0	31.0	34.0	30.0	34.0
2	32.44197493054492	34.0	31.0	34.0	31.0	34.0
3	32.51414987713196	34.0	31.0	34.0	31.0	34.0
4	35.99421754570181	37.0	35.0	37.0	35.0	37.0
5	35.10277164045239	37.0	35.0	37.0	35.0	37.0
6	26.113417773208205	36.0	0.0	37.0	0.0	37.0
7	31.035854133397503	37.0	19.0	37.0	17.0	37.0
8	34.60211901793071	37.0	32.0	37.0	32.0	37.0
9	37.058043299134454	39.0	37.0	39.0	34.0	39.0
10	37.25672492872195	39.0	37.0	39.0	34.0	39.0
11	37.33496671260546	39.0	37.0	39.0	34.0	39.0
12	37.08363776897892	39.0	37.0	39.0	34.0	39.0
13	37.04971598159531	39.0	37.0	39.0	33.0	39.0
14	37.998884343622166	40.0	37.0	41.0	33.0	41.0
15	38.166057795375494	40.0	37.0	41.0	33.0	41.0
16	38.31989441369705	40.0	37.0	41.0	34.0	41.0
17	38.329614478740545	40.0	37.0	41.0	34.0	41.0
18	38.3439284229869	40.0	37.0	41.0	34.0	41.0
19	38.34955774797833	40.0	37.0	41.0	34.0	41.0
20	38.26606581643442	40.0	37.0	41.0	34.0	41.0
21	38.17040375093883	40.0	37.0	41.0	34.0	41.0
22	38.19755868133791	40.0	37.0	41.0	34.0	41.0
23	38.163673353313065	40.0	37.0	41.0	34.0	41.0
24	38.08031267546066	40.0	37.0	41.0	34.0	41.0
25	37.94383800377719	40.0	36.0	41.0	34.0	41.0
26	37.87772989448661	40.0	36.0	41.0	34.0	41.0
27	37.800166254675915	40.0	36.0	41.0	33.0	41.0
28	37.75669940717083	40.0	36.0	41.0	33.0	41.0
29	37.70131034935358	40.0	36.0	41.0	33.0	41.0
30	37.448406361428916	40.0	36.0	41.0	33.0	41.0
31	37.48614908960981	40.0	36.0	41.0	33.0	41.0
32	37.43939360794522	39.0	35.0	41.0	33.0	41.0
33	37.36486338678275	39.0	35.0	41.0	33.0	41.0
34	37.321214242483904	39.0	35.0	41.0	32.0	41.0
35	37.31640889900029	40.0	35.0	41.0	32.0	41.0
36	37.224990702863515	39.0	35.0	41.0	32.0	41.0
37	37.13901224305267	39.0	35.0	41.0	32.0	41.0
38	37.065583094524534	39.0	35.0	41.0	32.0	41.0
39	37.01319828786851	39.0	35.0	41.0	32.0	41.0
40	36.880770605006596	39.0	35.0	41.0	31.0	41.0
41	36.76357564223161	39.0	35.0	41.0	31.0	41.0
42	36.79450047032573	39.0	35.0	41.0	31.0	41.0
43	36.72681002486528	39.0	35.0	41.0	31.0	41.0
44	36.57925170812096	39.0	35.0	40.0	31.0	41.0
45	36.55596146974967	39.0	35.0	40.0	31.0	41.0
46	36.479761409956325	38.0	35.0	40.0	31.0	41.0
47	36.39753097222526	38.0	35.0	40.0	31.0	41.0
48	36.39174851792707	38.0	35.0	40.0	31.0	41.0
49	36.39112870882827	38.0	35.0	40.0	31.0	41.0
50	36.28743829253531	38.0	35.0	40.0	31.0	41.0
51	35.26036357272548	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	6.0
12	1.0
13	2.0
14	6.0
15	7.0
16	8.0
17	15.0
18	31.0
19	53.0
20	90.0
21	174.0
22	230.0
23	342.0
24	450.0
25	636.0
26	813.0
27	962.0
28	1215.0
29	1552.0
30	2064.0
31	2758.0
32	4042.0
33	6351.0
34	9362.0
35	11248.0
36	13892.0
37	22457.0
38	29555.0
39	28800.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.99114766769482	4.811906168194314	51.92760629726044	17.269339866850423
2	21.19236686865151	5.405464528689869	49.975572229635624	23.426596373022992
3	22.1928116728283	5.245043350177557	49.487746009523185	23.07439896747096
4	20.788397173670507	4.674089792108736	48.2240646351512	26.313448399069557
5	18.754694142439423	8.35648502614136	47.47081428331838	25.41800654810083
6	16.827452438766507	31.215773776970813	38.404100948672514	13.552672835590169
7	79.18753964955265	2.695075799006847	11.872625584261225	6.244758967179285
8	78.7660694623703	3.7057292236344144	10.035073903120193	7.493127410875097
9	72.48412194926316	5.896207497502534	13.32808318567293	8.291587367561378
10	38.90359416358585	24.18057591203086	20.512764421499355	16.403065502883933
11	28.453612757858814	24.147033301978286	26.429389159903454	20.969964780259446
12	25.45592428120374	21.333099993437315	32.86811191564763	20.342863809711314
13	24.981952617417367	23.008042934540867	32.4065364338372	19.603468014204566
14	18.90928182355129	25.474883147755197	34.01731090353583	21.598524125157688
15	17.103085190937662	24.88642909748503	36.2697700872837	21.7407156242936
16	21.861031508177835	25.312274407717716	29.061754861855487	23.76493922224896
17	22.04770342499216	24.503605830580653	27.388999482277104	26.059691262150082
18	23.514098834029706	22.977417073188516	30.908785976272252	22.599698116509526
19	23.376282457944132	24.398602877372593	28.716849328054018	23.508265336629258
20	23.682541071467636	27.46921007153326	29.023107941577525	19.825140915421578
21	23.65118602294023	25.983126608769204	30.929932404348868	19.435754963941694
22	22.041869927591716	23.26763356886079	30.567526378346056	24.122970125201437
23	21.855927197952443	24.391311005622033	29.46207862096121	24.29068317546431
24	23.26034169711023	24.962993750865913	27.14763852733358	24.62902602469028
25	21.976972269011732	25.350192140820628	25.768016392127695	26.90481919803995
26	19.856495963948987	26.26094692246553	25.776037451053313	28.106519662532175
27	20.287445584407063	26.885131144313434	28.63663873879786	24.190784532481644
28	16.764742341711695	25.542697555035403	29.754482678158656	27.93807742509425
29	18.729172591312466	26.79544112178155	27.076178184178097	27.39920810272789
30	20.917463303655413	27.341602315898466	27.946827671194917	23.7941067092512
31	20.68485259481256	26.7101262223	27.952661168595366	24.652360014292068
32	21.484770925848956	25.974376362668533	24.665485383443077	27.875367328039435
33	21.522688658951868	25.733015407725006	25.74541158970096	26.998884343622166
34	18.1224888616659	23.359511152917843	30.310852492726358	28.2071474926899
35	18.70948453758595	24.809135256929103	26.017398405996833	30.46398179948811
36	22.00686894318903	25.981668234419093	26.088858749152323	25.922604073239558
37	20.10514879064307	26.392200613975604	28.972064839323608	24.53058575605772
38	19.74930544921576	27.076178184178097	27.074719809827986	26.09979655677816
39	21.127469210071535	24.510168515156156	27.4436885204063	26.918673754366008
40	23.01825155499165	24.01577961046821	27.617235068069622	25.348733766470517
41	18.608856707428227	23.828378506478828	26.181465520384428	31.381299265708513
42	20.13577465199542	23.196902412880362	26.913569444140617	29.753753490983602
43	20.98381933658551	22.73678530542005	28.217356113140685	28.06203924485376
44	21.174866376450172	22.39771326901902	28.11672828298296	28.310692071547845
45	20.722041140740416	22.303648123436805	26.442514529054463	30.531796206768313
46	20.877358009027336	23.391595388620303	29.54301839739243	26.188028204959927
47	16.885058225595927	24.267349185862518	32.416745054287986	26.430847534253566
48	17.569764982973478	24.44964597962651	30.149702127038992	27.83088691036102
49	21.271119083557558	21.49133361042446	31.57088793122307	25.666659374794914
50	20.64183055148426	21.723944319267314	28.81966471973691	28.814560409511518
51	19.037618766361135	22.18916573695302	26.697000853148996	32.07621464353685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	90.5
2	147.0
3	297.0
4	447.0
5	332.0
6	217.0
7	224.0
8	231.0
9	278.0
10	325.0
11	347.5
12	370.0
13	371.5
14	373.0
15	366.5
16	360.0
17	360.0
18	360.0
19	377.5
20	395.0
21	406.0
22	417.0
23	491.0
24	565.0
25	657.5
26	863.0
27	976.0
28	1149.0
29	1322.0
30	1529.5
31	1737.0
32	2027.0
33	2317.0
34	2686.5
35	3056.0
36	3344.5
37	3633.0
38	3817.0
39	4001.0
40	4699.5
41	5398.0
42	6334.0
43	7270.0
44	8359.0
45	9448.0
46	11265.0
47	13082.0
48	15065.5
49	17049.0
50	15835.0
51	14621.0
52	12289.0
53	9957.0
54	9109.0
55	8261.0
56	7710.5
57	7160.0
58	6707.0
59	6254.0
60	5593.5
61	4933.0
62	4282.5
63	3632.0
64	3093.5
65	2555.0
66	2273.5
67	1992.0
68	1707.5
69	1423.0
70	1164.0
71	905.0
72	729.0
73	553.0
74	433.5
75	248.0
76	182.0
77	126.5
78	71.0
79	48.5
80	26.0
81	18.0
82	10.0
83	7.5
84	5.0
85	3.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	137139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.73951246545476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47195805274251	41.687630797949524
2	12.217909833958771	13.864764946514121
3	5.939957847118697	10.110909369326013
4	3.329820593224696	7.557295882280022
5	2.0009767131033773	5.676722157810688
6	1.1861923610754126	4.038238575459935
7	0.6528556006785585	2.592989594499012
8	0.32128720505834574	1.4583743501119302
9	0.20819410887780804	1.0631549012315973
>10	0.6078753919703902	5.846622769598729
>50	0.033413869326067956	1.2724316204726591
>100	0.024417827584434275	2.531737871794311
>500	0.005140595280933532	2.299127162951458
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT	924	0.6737689497517118	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	896	0.6533517088501447	TruSeq Adapter, Index 22 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	751	0.5476195684670299	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	582	0.42438693588257176	TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	264	0.1925054142147748	No Hit
CCTGTATCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	262	0.19104703986466284	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT	261	0.1903178526896069	TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	248	0.18083841941387935	No Hit
TCTGTATCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	240	0.17500492201343162	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	236	0.17208817331320778	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	208	0.15167093241164076	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.12687856845973794	No Hit
GCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	173	0.12614938128468195	No Hit
GCTGTATCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	169	0.1232326325844581	TruSeq Adapter, Index 20 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	169	0.1232326325844581	No Hit
TTCCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	165	0.12031588388423424	No Hit
TGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	158	0.11521157365884248	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	144	0.10500295320805897	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7233536776555174	0.0
2	0.0	0.0	0.0	2.490903389991177	0.0
3	0.0	0.0	0.0	3.487702258292681	0.0
4	0.0	0.0	0.0	4.619400753979539	0.0
5	0.0	0.0	0.0	6.2629886465556845	0.0
6	0.0	0.0	0.0	7.142388379673179	0.0
7	0.0	0.0	0.0	8.173459045202312	0.0
8	0.0	0.0	0.0	9.536309875381912	0.0
9	0.0	0.0	0.0	10.166327594630266	0.0
10	0.0	0.0	0.0	11.512407119783576	0.0
11	0.0	0.0	0.0	13.771428988106956	0.0
12	0.0	0.0	0.0	15.84377893961601	0.0
13	0.0	0.0	0.0	16.55911155834591	0.0
14	0.0	0.0	0.0	16.86682854621953	0.0
15	0.0	0.0	0.0	17.305070038428163	0.0
16	0.0	0.0	0.0	18.236242060974632	0.0
17	0.0	0.0	0.0	19.558258409351097	0.0
18	0.0	0.0	0.0	21.166845317524555	0.0
19	0.0	0.0	0.0	22.014160814939586	0.0
20	0.0	0.0	0.0	22.95335389641167	0.0
21	0.0	0.0	0.0	23.893276165058808	0.0
22	0.0	0.0	0.0	24.84340705415673	0.0
23	0.0	0.0	0.0	25.63895026214279	0.0
24	0.0	0.0	0.0	26.24927992766463	0.0
25	0.0	0.0	0.0	26.792524373081328	0.0
26	0.0	0.0	0.0	27.248266357491303	0.0
27	0.0	0.0	0.0	27.810469669459454	0.0
28	0.0	0.0	0.0	28.347151430300645	0.0
29	0.0	0.0	0.0	28.896229373117787	0.0
30	0.0	0.0	0.0	29.483954236212895	0.0
31	0.0	0.0	0.0	29.96886370762511	0.0
32	0.0	0.0	0.0	30.41148032288408	0.0
33	0.0	0.0	0.0	30.892014671245963	0.0
34	0.0	0.0	0.0	31.444009362763328	0.0
35	0.0	0.0	0.0	31.96537819292834	0.0
36	0.0	0.0	0.0	32.40143212361181	0.0
37	0.0	0.0	0.0	32.863736792597294	0.0
38	0.0	0.0	0.0	33.35083382553468	0.0
39	0.0	0.0	0.0	34.020956839411106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGT	30	2.1514898E-6	45.000004	30
AGGGCAC	30	2.1514898E-6	45.000004	6
CTGTCGG	20	7.0115447E-4	45.0	2
TCGTTAG	25	3.8727943E-5	45.0	1
ACACGCG	45	3.8016879E-10	45.0	36
CTCGTAA	35	1.201497E-7	45.0	33
CGCGGTA	20	7.0115447E-4	45.0	30
AGATAAA	35	1.201497E-7	45.0	18
TTGCTAG	35	1.201497E-7	45.0	1
TGGGTCC	25	3.8727943E-5	45.0	6
GACAAAC	20	7.0115447E-4	45.0	12
ACTCTAC	20	7.0115447E-4	45.0	11
CACGCGG	50	2.1827873E-11	45.0	37
GTCTAGG	20	7.0115447E-4	45.0	2
TAAAACG	35	1.201497E-7	45.0	21
ACTTAGG	20	7.0115447E-4	45.0	2
GCGTTAG	45	3.8016879E-10	45.0	1
AAAACGA	35	1.201497E-7	45.0	22
GCACCGA	35	1.201497E-7	45.0	9
CGTTAGG	75	0.0	45.0	2
>>END_MODULE