FastQCFastQC Report
Sat 14 Jan 2017
SRR2934234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366982
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC21640.5896746979415884TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT19680.5362660838951229TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.522641437454698No Hit
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC16280.4436184881002338TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC15740.42890386994457497TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.19810235924377761No Hit
TCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG6230.1697630946476939TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC5950.16213329264105597TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC5800.1580458987089285TruSeq Adapter, Index 16 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT5590.15232354720395005TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG5340.14551122398373761TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC5060.13788142197709968TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC4790.13052411289927027No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG4670.12725419775356828No Hit
TTCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT4220.1149920159571859No Hit
TGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG4090.11144960788267544TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG4070.11090462202505845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGT302.161447E-645.00000413
AACCGAT302.161447E-645.00000433
GTCGATC302.161447E-645.00000445
CGACGTT302.161447E-645.00000427
CACGCTC302.161447E-645.00000413
AAGCGTG302.161447E-645.00000412
ATCACGC302.161447E-645.00000411
TCGTCAG302.161447E-645.0000041
GGCATAC302.161447E-645.0000048
TCGACGT302.161447E-645.00000426
CCTTCGA207.027003E-445.011
TATTTCA207.027003E-445.035
CTTCGAA207.027003E-445.012
CTATGCA207.027003E-445.013
GGTACTT406.7939254E-945.09
AATCCAT253.8855982E-545.033
AGATCCG207.027003E-445.030
ACGCCGG207.027003E-445.027
GATAATA207.027003E-445.028
GATCGTT406.7939254E-945.010
Produced by FastQC (version 0.11.5)