##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366982
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.269184864652765	33.0	31.0	34.0	30.0	34.0
2	32.44101073077154	34.0	31.0	34.0	31.0	34.0
3	32.49330212380989	34.0	31.0	34.0	30.0	34.0
4	35.997065251156734	37.0	35.0	37.0	35.0	37.0
5	35.1432331830989	37.0	35.0	37.0	35.0	37.0
6	26.209416265647906	36.0	0.0	37.0	0.0	37.0
7	31.15974897951398	37.0	19.0	37.0	17.0	37.0
8	34.721253358475344	37.0	32.0	37.0	32.0	37.0
9	37.21260988277354	39.0	37.0	39.0	34.0	39.0
10	37.35574496841807	39.0	37.0	39.0	34.0	39.0
11	37.38075164449482	39.0	37.0	39.0	35.0	39.0
12	36.97320032045168	39.0	35.0	39.0	34.0	39.0
13	36.864699086058714	39.0	35.0	39.0	33.0	39.0
14	37.9232959654697	40.0	36.0	41.0	33.0	41.0
15	38.0696382928863	40.0	36.0	41.0	33.0	41.0
16	38.17862183976326	40.0	36.0	41.0	34.0	41.0
17	38.15926666702999	40.0	36.0	41.0	34.0	41.0
18	38.15168318882125	40.0	36.0	41.0	34.0	41.0
19	38.13664975393888	40.0	36.0	41.0	34.0	41.0
20	38.048288471914155	40.0	36.0	41.0	34.0	41.0
21	37.9327405703822	40.0	36.0	41.0	34.0	41.0
22	37.91458709146497	40.0	35.0	41.0	34.0	41.0
23	37.88713615381681	39.0	35.0	41.0	34.0	41.0
24	37.81632886626592	39.0	35.0	41.0	34.0	41.0
25	37.707726809489294	39.0	35.0	41.0	34.0	41.0
26	37.68240676654441	39.0	35.0	41.0	34.0	41.0
27	37.65491495495692	39.0	35.0	41.0	34.0	41.0
28	37.63397932323656	39.0	35.0	41.0	34.0	41.0
29	37.65260148999133	39.0	35.0	41.0	34.0	41.0
30	37.38057997394968	39.0	35.0	41.0	33.0	41.0
31	37.38286073976381	39.0	35.0	41.0	33.0	41.0
32	37.27083617180134	39.0	35.0	41.0	33.0	41.0
33	37.15859360949583	39.0	35.0	41.0	33.0	41.0
34	37.04410570545694	39.0	35.0	41.0	32.0	41.0
35	37.003376187387936	39.0	35.0	41.0	32.0	41.0
36	36.94731621714416	39.0	35.0	41.0	32.0	41.0
37	36.905859142955244	39.0	35.0	41.0	32.0	41.0
38	36.834092680294944	39.0	35.0	41.0	32.0	41.0
39	36.81227689641454	39.0	35.0	41.0	32.0	41.0
40	36.75400428358884	39.0	35.0	41.0	32.0	41.0
41	36.675112130840205	39.0	35.0	41.0	31.0	41.0
42	36.60367265969448	39.0	35.0	41.0	31.0	41.0
43	36.492533693750644	39.0	35.0	40.0	31.0	41.0
44	36.37845997896355	39.0	35.0	40.0	31.0	41.0
45	36.36732046803385	39.0	35.0	40.0	31.0	41.0
46	36.29601724335254	38.0	35.0	40.0	31.0	41.0
47	36.22246322707926	38.0	35.0	40.0	31.0	41.0
48	36.22325073164351	38.0	35.0	40.0	31.0	41.0
49	36.2256323198413	38.0	35.0	40.0	31.0	41.0
50	36.10218757323248	38.0	35.0	40.0	31.0	41.0
51	35.130464164454935	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	3.0
12	1.0
13	6.0
14	5.0
15	4.0
16	18.0
17	21.0
18	50.0
19	126.0
20	185.0
21	309.0
22	533.0
23	849.0
24	1370.0
25	2081.0
26	2879.0
27	3521.0
28	3750.0
29	4502.0
30	5519.0
31	7488.0
32	10391.0
33	18303.0
34	28103.0
35	36947.0
36	32589.0
37	53261.0
38	76147.0
39	77978.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.073066253930712	4.190668752145882	53.51243385234154	19.223831141581876
2	21.851480454082218	4.789063223809342	51.840689734101396	21.51876658800704
3	19.498504013820842	3.9522374394384467	53.122768964145386	23.42648958259533
4	18.972592661220443	4.637284662463009	52.84264623333025	23.547476442986305
5	17.62375266361838	7.660593707593288	50.02806677166728	24.687586857121058
6	16.50435171207307	31.083540882114107	39.27822073017205	13.133886675640769
7	79.367652909407	1.9987356328103285	12.937964259827458	5.695647197955213
8	79.78347711876876	2.7870576758533114	10.55310614689549	6.876359058482433
9	73.6594710367266	4.975720880043163	13.626008905068915	7.738799178161326
10	42.79065458251358	23.622139505479833	19.643197759018154	13.94400815298843
11	31.489282853109962	22.143592873764923	27.225041010185784	19.142083262939327
12	30.667989165681153	19.19957927091792	30.795788349292337	19.33664321410859
13	25.34402232262073	24.7690622428348	31.13967442544866	18.747241009095813
14	18.862778010910617	27.49290155920454	31.830443999978204	21.813876429906646
15	16.135941272323983	24.451880473701706	39.548806208478894	19.86337204549542
16	19.343455537328808	22.36594710367266	36.540211781504276	21.750385577494264
17	19.122191279136306	23.28533824547253	30.250529998746533	27.341940476644634
18	21.286330119733393	22.98804846014246	33.63243973818879	22.093181681935352
19	21.7035167937392	25.847589255058836	31.366661035146137	21.08223291605583
20	24.739905499452288	25.264181894479837	31.602639911494297	18.393272694573575
21	23.16762130022726	27.250655345493787	30.20039129984577	19.381332054433187
22	19.745655100250147	25.498798306183957	30.163332261527813	24.592214332038083
23	20.559046492743512	25.743496956253985	29.29925718427607	24.398199366726434
24	22.615278133532435	22.46213710754206	31.359303726068312	23.563281032857198
25	19.666359657966876	23.65374868522162	30.077769481881944	26.60212217492956
26	17.401125940781835	26.18520799385256	31.98304004011096	24.430626025254647
27	16.995111476857176	26.344071371347916	33.71827501076347	22.942542141031442
28	14.512700895411765	24.63690317236268	35.030328462976385	25.820067469249175
29	16.76049506515306	21.919876179213148	34.0398166667575	27.279812088876294
30	19.361985056487786	23.986190058367985	31.919821680627386	24.732003204516843
31	21.38987743268062	24.532810873557832	26.9688976571058	27.10841403665575
32	20.19118103885204	25.61406281506995	29.197344828901688	24.99741131717632
33	21.102942378645274	24.73282068330327	27.63051048825283	26.53372644979863
34	16.840607986222757	23.74857622444698	31.177278449624236	28.233537339706032
35	15.801592448675958	22.82836760386068	33.55178183126148	27.818258116201882
36	18.81291180493866	23.8872751252105	32.536200685592206	24.76361238425863
37	16.698094184455915	26.365053326866168	31.478110643028813	25.458741845649108
38	16.841152972080376	26.510019564992287	27.59399643579249	29.05483102713485
39	18.729256475794454	24.290837152775886	28.46869873726777	28.511207634161895
40	20.448959349504882	22.09617910415225	29.252660893449818	28.202200652893055
41	16.395082047620864	22.865699135107445	30.710770555504084	30.02844826176761
42	17.95019919233096	23.93632385239603	30.54482236185971	27.5686545934133
43	18.981312434942314	23.28888065354704	30.04479783749612	27.685009074014527
44	17.51884288602711	22.040863039604123	29.543683341417292	30.89661073295148
45	18.790839877705174	22.237875427132668	27.858042083807927	31.113242611354234
46	19.587881694470028	22.471674360050358	30.705320696927913	27.2351232485517
47	15.139979617528926	22.771416581739704	33.655601637137515	28.433002163593855
48	16.237308641840745	20.817642282182778	34.18832531295813	28.75672376301835
49	18.372290739055323	19.426293387686588	34.42130676708939	27.780109106168695
50	18.470933179283996	19.733392918453767	31.410532396684303	30.385141505577927
51	16.741693053065273	19.98517638467282	28.91667711222894	34.35645345003297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	186.0
2	309.0
3	2288.5
4	4268.0
5	2688.5
6	1109.0
7	1133.0
8	1157.0
9	1294.0
10	1431.0
11	1452.5
12	1474.0
13	1424.5
14	1375.0
15	1329.5
16	1284.0
17	1141.0
18	998.0
19	991.0
20	984.0
21	1037.5
22	1091.0
23	1247.5
24	1404.0
25	1516.5
26	2068.0
27	2507.0
28	2638.0
29	2769.0
30	3552.0
31	4335.0
32	5005.5
33	5676.0
34	6859.5
35	8043.0
36	8569.0
37	9095.0
38	10090.0
39	11085.0
40	13792.0
41	16499.0
42	18829.0
43	21159.0
44	25195.0
45	29231.0
46	33994.5
47	38758.0
48	43971.0
49	49184.0
50	46488.0
51	43792.0
52	35986.0
53	28180.0
54	23809.5
55	19439.0
56	17251.0
57	15063.0
58	13956.0
59	12849.0
60	11896.0
61	10943.0
62	9427.5
63	7912.0
64	6460.5
65	5009.0
66	3964.0
67	2919.0
68	2282.0
69	1645.0
70	1400.5
71	1156.0
72	866.0
73	576.0
74	434.0
75	211.0
76	130.0
77	95.0
78	60.0
79	51.0
80	42.0
81	25.0
82	8.0
83	23.0
84	38.0
85	22.5
86	7.0
87	4.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	366982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.485745664319744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.68768833578838	25.79093018218276
2	12.236584387146895	8.929218114988549
3	4.806255410143711	5.260794374767926
4	2.515447374560977	3.6711189256085053
5	1.5117595030039221	2.757883636610976
6	1.0740977596156336	2.3513554605570994
7	0.8243270844802619	2.1053331843989933
8	0.6637577464745079	1.9374157056472738
9	0.5663098174503756	1.859601237003157
>10	4.92426304013355	35.44220246914413
>50	0.1363270217340743	3.326562819187672
>100	0.04419392462807903	2.911972300233704
>500	0.0052433469897720875	1.1270740277957576
>1k	0.0037452478498372055	2.528537561873508
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2164	0.5896746979415884	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	1968	0.5362660838951229	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1918	0.522641437454698	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	1628	0.4436184881002338	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	1574	0.42890386994457497	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	727	0.19810235924377761	No Hit
TCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	623	0.1697630946476939	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	595	0.16213329264105597	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	580	0.1580458987089285	TruSeq Adapter, Index 16 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	559	0.15232354720395005	TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	534	0.14551122398373761	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	506	0.13788142197709968	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	479	0.13052411289927027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	467	0.12725419775356828	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	422	0.1149920159571859	No Hit
TGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	409	0.11144960788267544	TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	407	0.11090462202505845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5646053484912067	0.0
2	0.0	0.0	0.0	2.1540566022311722	0.0
3	0.0	0.0	0.0	3.0276689319912147	0.0
4	0.0	0.0	0.0	3.950874974794404	0.0
5	0.0	0.0	0.0	5.371108119744292	0.0
6	0.0	0.0	0.0	6.175234752658168	0.0
7	0.0	0.0	0.0	7.261664059817647	0.0
8	0.0	0.0	0.0	9.017880985988414	0.0
9	0.0	0.0	0.0	9.880866091524926	0.0
10	0.0	0.0	0.0	11.482579527061272	0.0
11	0.0	0.0	0.0	14.451389986429852	0.0
12	0.0	0.0	0.0	17.113373407960065	0.0
13	0.0	0.0	0.0	17.893248170209983	0.0
14	0.0	0.0	0.0	18.185905575750308	0.0
15	0.0	0.0	0.0	18.571755562943142	0.0
16	0.0	0.0	0.0	19.56362982380607	0.0
17	0.0	0.0	0.0	20.99994005155566	0.0
18	0.0	0.0	0.0	22.60029102244797	0.0
19	0.0	0.0	0.0	23.66819081044847	0.0
20	0.0	0.0	0.0	24.824105814454114	0.0
21	0.0	0.0	0.0	26.258781084630854	0.0
22	0.0	0.0	0.0	27.664299611425083	0.0
23	0.0	0.0	0.0	28.859181104250343	0.0
24	0.0	0.0	0.0	29.813451340937704	0.0
25	0.0	0.0	0.0	30.67888888283349	0.0
26	0.0	0.0	0.0	31.4519513218632	0.0
27	0.0	0.0	0.0	32.230736112397885	0.0
28	0.0	0.0	0.0	32.957202260601335	0.0
29	0.0	0.0	0.0	33.7384394874953	0.0
30	0.0	0.0	0.0	34.64992833435972	0.0
31	0.0	0.0	0.0	35.423535759247045	0.0
32	0.0	0.0	0.0	36.226027434588076	0.0
33	0.0	0.0	0.0	36.93505403534778	0.0
34	0.0	0.0	0.0	37.71383882588247	0.0
35	0.0	0.0	0.0	38.491806137630725	0.0
36	0.0	0.0	0.0	39.16949605157746	0.0
37	0.0	0.0	0.0	39.924301464377	0.0
38	2.7249292880849745E-4	0.0	0.0	40.67665444081726	0.0
39	2.7249292880849745E-4	0.0	0.0	41.50339798682224	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTGT	30	2.161447E-6	45.000004	13
AACCGAT	30	2.161447E-6	45.000004	33
GTCGATC	30	2.161447E-6	45.000004	45
CGACGTT	30	2.161447E-6	45.000004	27
CACGCTC	30	2.161447E-6	45.000004	13
AAGCGTG	30	2.161447E-6	45.000004	12
ATCACGC	30	2.161447E-6	45.000004	11
TCGTCAG	30	2.161447E-6	45.000004	1
GGCATAC	30	2.161447E-6	45.000004	8
TCGACGT	30	2.161447E-6	45.000004	26
CCTTCGA	20	7.027003E-4	45.0	11
TATTTCA	20	7.027003E-4	45.0	35
CTTCGAA	20	7.027003E-4	45.0	12
CTATGCA	20	7.027003E-4	45.0	13
GGTACTT	40	6.7939254E-9	45.0	9
AATCCAT	25	3.8855982E-5	45.0	33
AGATCCG	20	7.027003E-4	45.0	30
ACGCCGG	20	7.027003E-4	45.0	27
GATAATA	20	7.027003E-4	45.0	28
GATCGTT	40	6.7939254E-9	45.0	10
>>END_MODULE