##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934233.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1775137
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38768162682655	33.0	31.0	34.0	31.0	34.0
2	32.58443658151455	34.0	31.0	34.0	31.0	34.0
3	32.567599571188026	34.0	31.0	34.0	31.0	34.0
4	36.076827309666804	37.0	35.0	37.0	35.0	37.0
5	35.172088689492696	37.0	35.0	37.0	35.0	37.0
6	25.886029641655828	37.0	0.0	37.0	0.0	37.0
7	30.997541034860973	37.0	19.0	37.0	17.0	37.0
8	34.76813789583564	37.0	32.0	37.0	32.0	37.0
9	37.305725135581085	39.0	37.0	39.0	35.0	39.0
10	37.4932137632194	39.0	37.0	39.0	35.0	39.0
11	37.529475189802255	39.0	37.0	39.0	35.0	39.0
12	37.18018834602625	39.0	37.0	39.0	34.0	39.0
13	37.166129712805265	39.0	37.0	39.0	34.0	39.0
14	38.12208297162416	40.0	38.0	41.0	33.0	41.0
15	38.31534185812137	40.0	38.0	41.0	33.0	41.0
16	38.46125115976964	40.0	38.0	41.0	34.0	41.0
17	38.46281441939411	40.0	38.0	41.0	34.0	41.0
18	38.37375368774354	40.0	37.0	41.0	34.0	41.0
19	38.315652820035865	40.0	37.0	41.0	34.0	41.0
20	38.16771099920739	40.0	36.0	41.0	34.0	41.0
21	38.044308692793855	40.0	36.0	41.0	34.0	41.0
22	38.053997522444746	40.0	36.0	41.0	34.0	41.0
23	37.99538570825801	40.0	35.0	41.0	34.0	41.0
24	37.95307235441546	40.0	35.0	41.0	34.0	41.0
25	37.84030979017394	40.0	35.0	41.0	34.0	41.0
26	37.799076916316885	40.0	35.0	41.0	34.0	41.0
27	37.76357993777382	40.0	35.0	41.0	34.0	41.0
28	37.67751559457101	40.0	35.0	41.0	34.0	41.0
29	37.61114156259489	40.0	35.0	41.0	33.0	41.0
30	37.33026915669044	39.0	35.0	41.0	33.0	41.0
31	37.25859806876878	39.0	35.0	41.0	33.0	41.0
32	37.09364291319487	39.0	35.0	41.0	32.0	41.0
33	36.87202227208379	39.0	35.0	41.0	32.0	41.0
34	36.59822537640757	39.0	35.0	41.0	31.0	41.0
35	36.46409939063858	39.0	35.0	41.0	31.0	41.0
36	36.37774999901416	39.0	35.0	41.0	30.0	41.0
37	36.30252256586393	39.0	35.0	41.0	30.0	41.0
38	36.214184595329826	39.0	35.0	41.0	30.0	41.0
39	36.12766338598091	39.0	35.0	41.0	30.0	41.0
40	36.02413560192819	39.0	35.0	41.0	29.0	41.0
41	35.8867890196644	39.0	35.0	41.0	29.0	41.0
42	35.834210542622905	39.0	35.0	41.0	29.0	41.0
43	35.76951581765238	39.0	35.0	41.0	29.0	41.0
44	35.64476995296701	38.0	35.0	40.0	28.0	41.0
45	35.60252588955106	38.0	35.0	40.0	28.0	41.0
46	35.56960786688577	38.0	35.0	40.0	28.0	41.0
47	35.51244495495277	38.0	35.0	40.0	28.0	41.0
48	35.44658919283413	38.0	35.0	40.0	28.0	41.0
49	35.41131135230689	38.0	35.0	40.0	27.0	41.0
50	35.313302578899545	38.0	35.0	40.0	27.0	41.0
51	34.33764210874992	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	20.0
10	29.0
11	17.0
12	16.0
13	23.0
14	32.0
15	56.0
16	102.0
17	198.0
18	377.0
19	820.0
20	1383.0
21	2290.0
22	3649.0
23	5906.0
24	9916.0
25	16447.0
26	22789.0
27	25770.0
28	26048.0
29	26719.0
30	30419.0
31	37574.0
32	51614.0
33	84038.0
34	122032.0
35	136244.0
36	157981.0
37	258461.0
38	360988.0
39	392914.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.91575241798239	4.258713552813107	52.304808023268066	20.520726005936442
2	25.005900952996868	3.832436595034637	50.08790870789128	21.073753744077216
3	20.499375541155416	3.9859458734734274	53.66149204258601	21.85318654278515
4	18.237071279568845	4.530298224869404	52.92318283039562	24.309447665166125
5	18.085871682016656	7.747852700946462	50.60048886367644	23.565786753360445
6	15.984062075208843	31.423546464301065	39.49064213072005	13.10174932977004
7	79.92216938748953	2.029758829881863	13.502281795714921	4.545789986913686
8	81.58992798865665	2.252220532837747	12.059801581511737	4.098049896993866
9	76.02370971930618	4.189648460935691	14.323063515661044	5.463578304097092
10	41.392917842397516	24.066931172072916	20.477349072212455	14.062801913317113
11	31.03974510136401	21.09262552693116	29.85251279197042	18.015116579734407
12	28.50320848475357	19.47055354037463	32.48329565549025	19.542942319381545
13	26.26597271083866	18.550117540223656	35.130246285216295	20.053663463721392
14	19.83553945413791	20.32738881562381	38.360532173009744	21.47653955722854
15	18.70266914609971	21.917406938168714	38.761120972634785	20.618802943096785
16	24.792846974627874	22.326614790858397	33.9161428103859	18.96439542412783
17	24.73814697119152	23.156128231229477	32.454565478608124	19.651159318970873
18	24.433607096241023	22.421368040889238	33.596280174431605	19.548744688438134
19	23.914379566196864	24.908387352638133	31.24074367217854	19.93648940898646
20	23.316510218647913	28.242270878247705	30.142574911119535	18.298643991984846
21	23.72504206717566	25.730690081948605	32.82929711903926	17.71497073183647
22	22.440521492143986	22.34548657371234	34.47063522421087	20.74335670993281
23	21.3468030918177	23.44962670486841	33.955351051777974	21.24821915153591
24	21.6613140281567	24.414509978666434	32.42972232565712	21.494453667519746
25	23.499989014932368	24.774256860174738	29.670949340811443	22.05480478408145
26	21.481553254762872	24.378681758084024	30.49223806387901	23.64752692327409
27	21.01049102125639	23.354986122197893	33.60743424310349	22.02708861344223
28	20.217707140350292	24.395694529492655	31.282374261817537	24.104224068339512
29	22.032834648818657	25.32548192055036	30.324532698039647	22.317150732591344
30	24.785185594125974	23.319214235295643	30.960370946017125	20.935229224561258
31	24.035553312223225	23.668595719654313	31.50680764357906	20.789043324543403
32	26.664195495896937	23.427262233844488	29.120681952998556	20.78786031726002
33	26.652252755702797	23.511875421446344	28.97190470369329	20.863967119157564
34	23.292850072980283	24.327474442817653	31.59654719607557	20.78312828812649
35	22.63768937270757	26.987325485300573	29.517045726611524	20.857939415380333
36	25.127187366383552	26.596426078663228	28.283957801566867	19.992428753386356
37	24.49557414441815	26.21786374798114	28.315391995096718	20.97117011250399
38	25.45814773732957	24.576469309129383	29.003226229862822	20.96215672367823
39	23.8833960421083	23.80931725269655	28.4553248566167	23.851961848578448
40	24.954468303009854	22.84437764521837	29.79910846317777	22.402045588594007
41	22.143417662974745	23.013547686741926	30.10370467180843	24.7393299784749
42	22.407847957650592	23.610628362768622	31.553339263392065	22.428184416188724
43	22.525529015506972	22.650589785464444	31.460219690085893	23.36366150894269
44	23.61395204989812	23.01377302146257	29.609207627354962	23.76306730128435
45	22.616902244728156	22.843701641056438	28.67463187348357	25.86476424073184
46	22.206173382674127	23.06774068705683	30.085283558395776	24.640802371873267
47	20.745666390819412	23.983275657033793	31.951674715810668	23.319383236336126
48	21.58323554745352	24.357331293302995	30.952146228713616	23.10728693052987
49	23.652315286087777	22.27625248079444	30.67954755041442	23.391884682703363
50	21.452766744200588	23.12666571650526	30.20820364850713	25.21236389078702
51	20.296517958895567	22.884487225493018	30.24915823398419	26.56983658162722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338.0
1	1155.0
2	1972.0
3	20841.5
4	39711.0
5	25649.5
6	11588.0
7	11053.5
8	10519.0
9	10541.5
10	10564.0
11	10297.5
12	10031.0
13	9586.5
14	9142.0
15	8533.0
16	7924.0
17	7417.5
18	6911.0
19	6784.0
20	6657.0
21	6828.0
22	6999.0
23	7307.0
24	7615.0
25	8826.5
26	12493.0
27	14948.0
28	17603.0
29	20258.0
30	23448.5
31	26639.0
32	29949.5
33	33260.0
34	37256.5
35	41253.0
36	44637.0
37	48021.0
38	51215.0
39	54409.0
40	59508.5
41	64608.0
42	71278.0
43	77948.0
44	88951.5
45	99955.0
46	119649.5
47	139344.0
48	156738.0
49	174132.0
50	172609.0
51	171086.0
52	154412.0
53	137738.0
54	124554.5
55	111371.0
56	104411.0
57	97451.0
58	92226.5
59	87002.0
60	78465.5
61	69929.0
62	61716.0
63	53503.0
64	46210.5
65	38918.0
66	32731.5
67	26545.0
68	22788.5
69	19032.0
70	15401.0
71	11770.0
72	9492.0
73	7214.0
74	5750.5
75	3384.5
76	2482.0
77	1775.0
78	1068.0
79	797.0
80	526.0
81	400.5
82	275.0
83	175.0
84	75.0
85	61.5
86	48.0
87	35.0
88	22.0
89	15.0
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1775137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.190921610066574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88345444978786	26.000268538234096
2	8.721183106766262	6.138129421144967
3	3.2596118847084132	3.441262389420454
4	1.8828129540404408	2.6503169228822014
5	1.3733564980394817	2.416484043259148
6	1.1342808109256612	2.394983226065261
7	1.0285802622587208	2.533768116516585
8	0.900084906950673	2.5339853922324163
9	0.8073219266868634	2.55693623725128
>10	6.856344191379975	40.83373205456819
>50	0.09424405152341254	2.270463519079465
>100	0.055043156084008754	3.4052616675749143
>500	0.0011045402544674877	0.27597550552191125
>1k	0.0023931705513462235	1.4133703295707836
>5k	0.0	0.0
>10k+	1.8409004241124794E-4	1.1350626366782646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17521	0.9870224101013049	No Hit
CGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	2308	0.13001813381164384	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	2289	0.12894779388858438	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	2235	0.12590577515988907	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	2085	0.11745572313573543	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	2008	0.11311802976333657	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	1945	0.10956900791319205	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.633368016102419E-5	0.0	0.0	0.1322714810180848	0.0
2	5.633368016102419E-5	0.0	0.0	0.4037998193942214	0.0
3	5.633368016102419E-5	0.0	0.0	0.677750506017282	0.0
4	5.633368016102419E-5	0.0	0.0	0.9977821430120605	0.0
5	5.633368016102419E-5	0.0	0.0	1.6839263673733351	0.0
6	5.633368016102419E-5	0.0	0.0	2.665822412579987	0.0
7	5.633368016102419E-5	0.0	0.0	3.844097666827969	0.0
8	5.633368016102419E-5	0.0	0.0	5.436031134498351	0.0
9	5.633368016102419E-5	0.0	0.0	6.722861390416627	0.0
10	5.633368016102419E-5	0.0	0.0	8.149061171053276	0.0
11	5.633368016102419E-5	0.0	0.0	9.82628383048745	0.0
12	1.1266736032204839E-4	0.0	0.0	10.993348682383388	0.0
13	1.1266736032204839E-4	0.0	0.0	11.455904530185558	0.0
14	1.1266736032204839E-4	0.0	0.0	11.815482410653376	0.0
15	1.1266736032204839E-4	0.0	0.0	12.055407554459176	0.0
16	1.1266736032204839E-4	0.0	0.0	12.402873693692374	0.0
17	1.1266736032204839E-4	0.0	0.0	12.860359510280052	0.0
18	1.1266736032204839E-4	0.0	0.0	13.325844709450594	0.0
19	1.1266736032204839E-4	0.0	0.0	13.706322385258153	0.0
20	1.1266736032204839E-4	0.0	0.0	14.144091413789472	0.0
21	1.1266736032204839E-4	0.0	0.0	14.720497629197071	0.0
22	1.6900104048307257E-4	0.0	0.0	15.350533508117964	0.0
23	1.6900104048307257E-4	0.0	0.0	15.92063035134753	0.0
24	1.6900104048307257E-4	0.0	0.0	16.384538207473565	0.0
25	1.6900104048307257E-4	0.0	0.0	16.817124537430068	0.0
26	1.6900104048307257E-4	0.0	0.0	17.201038567727448	0.0
27	1.6900104048307257E-4	0.0	0.0	17.641229944505692	0.0
28	1.6900104048307257E-4	0.0	0.0	18.034664366750285	0.0
29	1.6900104048307257E-4	0.0	0.0	18.51017696098949	5.633368016102419E-5
30	2.2533472064409677E-4	0.0	0.0	19.129227772278984	5.633368016102419E-5
31	2.2533472064409677E-4	0.0	0.0	19.596909984975806	5.633368016102419E-5
32	2.2533472064409677E-4	0.0	0.0	20.056367480369122	5.633368016102419E-5
33	2.2533472064409677E-4	0.0	0.0	20.501572554681694	5.633368016102419E-5
34	2.2533472064409677E-4	0.0	0.0	20.997252606418545	5.633368016102419E-5
35	2.2533472064409677E-4	0.0	0.0	21.540196615810498	5.633368016102419E-5
36	2.2533472064409677E-4	0.0	0.0	22.00624515178265	5.633368016102419E-5
37	2.2533472064409677E-4	0.0	0.0	22.499164853191612	5.633368016102419E-5
38	2.2533472064409677E-4	0.0	0.0	23.108751606214057	5.633368016102419E-5
39	2.2533472064409677E-4	0.0	0.0	24.324432424088958	5.633368016102419E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	3630	0.0	44.2562	1
CGTTTTT	7970	0.0	43.30615	1
CGTTAGG	530	0.0	42.0283	2
TCGTTAG	265	0.0	41.603775	1
TTAGGGA	3395	0.0	40.493374	4
TTTAGGG	5930	0.0	40.219227	3
GTTTATT	3995	0.0	40.212765	2
TGCGTAG	140	0.0	40.17857	1
TAGGGAT	2095	0.0	40.059666	5
CGTAGGG	665	0.0	39.924812	3
GTTAGGG	2350	0.0	39.82979	3
TCGAGGG	1000	0.0	39.824997	3
TTAGGGG	5770	0.0	39.774696	4
CTTAGGG	2950	0.0	39.4322	3
TAGGGAG	2065	0.0	39.334137	5
GCTAGGG	1905	0.0	39.212597	3
TAGGGAC	1350	0.0	39.166668	5
TAGGGGA	2985	0.0	39.045227	5
CTAGGGA	2075	0.0	38.92771	4
CTTTAGG	1715	0.0	38.833817	2
>>END_MODULE