##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1699795
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37252256889801	33.0	31.0	34.0	31.0	34.0
2	32.54316785259399	34.0	31.0	34.0	31.0	34.0
3	32.59309446139093	34.0	31.0	34.0	31.0	34.0
4	36.05982721445822	37.0	35.0	37.0	35.0	37.0
5	35.18793266246812	37.0	35.0	37.0	35.0	37.0
6	26.230216584941125	37.0	0.0	37.0	0.0	37.0
7	31.184817580943584	37.0	19.0	37.0	17.0	37.0
8	34.79433461093838	37.0	32.0	37.0	32.0	37.0
9	37.32687765289344	39.0	37.0	39.0	35.0	39.0
10	37.39769678108242	39.0	37.0	39.0	35.0	39.0
11	37.41992357901982	39.0	37.0	39.0	35.0	39.0
12	37.082258154659826	39.0	37.0	39.0	34.0	39.0
13	37.054251247944606	39.0	37.0	39.0	33.0	39.0
14	37.87938839683609	40.0	37.0	41.0	33.0	41.0
15	38.10579040413697	40.0	37.0	41.0	33.0	41.0
16	38.30155518753732	40.0	37.0	41.0	33.0	41.0
17	38.32552925499839	40.0	37.0	41.0	34.0	41.0
18	38.32355666418597	40.0	37.0	41.0	34.0	41.0
19	38.32791777832033	40.0	37.0	41.0	34.0	41.0
20	38.22541365282284	40.0	37.0	41.0	34.0	41.0
21	38.13584991131283	40.0	37.0	41.0	34.0	41.0
22	38.19993705123265	40.0	37.0	41.0	34.0	41.0
23	38.16629240584894	40.0	36.0	41.0	34.0	41.0
24	38.121704087845885	40.0	36.0	41.0	34.0	41.0
25	38.00584894060754	40.0	36.0	41.0	34.0	41.0
26	37.96767316058701	40.0	36.0	41.0	34.0	41.0
27	37.93509629102333	40.0	36.0	41.0	34.0	41.0
28	37.874319550298715	40.0	36.0	41.0	34.0	41.0
29	37.847086266285054	40.0	36.0	41.0	34.0	41.0
30	37.58908633099874	40.0	35.0	41.0	33.0	41.0
31	37.5708288352419	40.0	35.0	41.0	33.0	41.0
32	37.544881588662165	40.0	35.0	41.0	33.0	41.0
33	37.46075967984375	40.0	35.0	41.0	33.0	41.0
34	37.344096788142096	40.0	35.0	41.0	32.0	41.0
35	37.2973299721437	40.0	35.0	41.0	32.0	41.0
36	37.210850720233914	40.0	35.0	41.0	32.0	41.0
37	37.142425998429225	39.0	35.0	41.0	32.0	41.0
38	37.10174697537056	39.0	35.0	41.0	32.0	41.0
39	37.066969840480766	39.0	35.0	41.0	32.0	41.0
40	36.98479169546916	39.0	35.0	41.0	32.0	41.0
41	36.85750163990364	39.0	35.0	41.0	31.0	41.0
42	36.84156971870137	39.0	35.0	41.0	31.0	41.0
43	36.78542059483644	39.0	35.0	41.0	31.0	41.0
44	36.66384181621901	39.0	35.0	41.0	31.0	41.0
45	36.60873752423086	39.0	35.0	41.0	31.0	41.0
46	36.55012104400825	39.0	35.0	41.0	31.0	41.0
47	36.45644445359587	39.0	35.0	40.0	31.0	41.0
48	36.408678105300936	39.0	35.0	40.0	31.0	41.0
49	36.40613956388859	39.0	35.0	40.0	31.0	41.0
50	36.305852764598086	38.0	35.0	40.0	31.0	41.0
51	35.26655096644007	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	10.0
10	15.0
11	12.0
12	17.0
13	10.0
14	31.0
15	47.0
16	79.0
17	148.0
18	323.0
19	533.0
20	993.0
21	1669.0
22	2554.0
23	3958.0
24	6023.0
25	8609.0
26	11686.0
27	13897.0
28	16065.0
29	19660.0
30	25325.0
31	34004.0
32	49601.0
33	80978.0
34	119237.0
35	127587.0
36	150778.0
37	255673.0
38	366011.0
39	404038.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.350109866189747	4.3173441503240095	56.20924876235075	16.12329722113549
2	20.632076220956055	4.733041337337738	52.91155698187134	21.723325459834864
3	20.99517883038837	4.698154777487874	51.808776940748736	22.49788945137502
4	19.28897308204813	4.39147073617701	51.73723890233822	24.582317279436637
5	18.658485287931782	7.762406643154026	49.38136657655776	24.197741492356432
6	16.201306628152217	31.0274474274839	39.49711582867346	13.274130115690422
7	84.51642698090063	1.9022882171085338	9.293708947255404	4.287575854735424
8	86.2100429757706	2.4653561164728686	7.281642786336	4.042958121420524
9	81.03994893501863	4.191211293126524	9.170223468124098	5.598616303730744
10	42.91223353404381	26.681040949055625	16.382740271620992	14.023985245279578
11	30.963498539529766	23.229977732608933	26.34064696036875	19.465876767492553
12	28.843713506628742	21.04583199738792	30.043681738091948	20.066772757891393
13	27.004315226247872	21.30804008718698	32.28236346147624	19.405281225088906
14	20.72520509826185	23.15826320232734	35.05893357728432	21.057598122126493
15	18.829917725372766	23.30633988216226	36.79502528246053	21.068717110004442
16	25.498545412829195	23.459417165011075	30.05733044278869	20.984706979371044
17	25.633620524827993	23.522601254857204	28.640218379275144	22.203559841039656
18	25.50466379769325	22.69226583205622	30.505619795328258	21.29745057492227
19	25.376060054300666	24.822346224103494	28.59280089657871	21.208792825017134
20	24.031603811047802	29.176400683611845	28.14133469035972	18.65066081498063
21	24.016425510135043	25.859706611679645	31.716059877808796	18.407808000376516
22	22.708503084195446	22.065072552866667	32.88531852370433	22.341105839233556
23	21.4158177897923	24.291105692156993	32.34625351880668	21.946822999244027
24	21.66502431175524	26.268873599463465	29.420783094431975	22.64531899434932
25	23.960771740121604	25.865648504672624	26.910951026447304	23.262628728758468
26	21.569777531996507	25.368647395715367	27.65162857874038	25.409946493547753
27	20.50753179059828	24.33181648375245	32.515273900676256	22.64537782497301
28	18.67266346824176	24.70462614609409	30.935259840157197	25.687450545506955
29	21.4174650472557	25.637856329733882	29.805888357125422	23.138790265885003
30	25.09249644810109	22.985183507422953	30.7021140784624	21.220205966013552
31	23.327460076067997	24.110672169291004	30.89290179109834	21.668965963542664
32	25.800993649234172	23.64791048332299	28.725640444877175	21.82545542256566
33	25.477601710794538	22.776981930173935	28.395247662218093	23.35016869681344
34	21.573072046923304	22.971475972102517	32.024155854088285	23.43129612688589
35	21.485355586997258	25.126677040466642	30.11151344720981	23.27645392532629
36	24.521486414538224	24.686918128362535	28.83200621251386	21.959589244585377
37	20.56565644680682	25.971367135448688	31.293420677199308	22.16955574054518
38	22.362167202515597	23.890645636679718	31.03185972426087	22.71532743654382
39	21.80562950238117	22.540071008562798	29.39760382869699	26.256695660359043
40	24.89288414191123	21.58489700228557	29.26747048908839	24.25474836671481
41	20.336981812512686	22.5664271279772	29.016499048414662	28.080092011095452
42	20.160725263928885	22.57472224591789	31.792539688609512	25.472012801543713
43	21.009651163816816	21.75544698037116	31.844545959953997	25.39035589585803
44	22.895231483796575	21.91864313049515	29.979732850137808	25.206392535570465
45	21.960412873317075	21.48217873331784	28.532852491035683	28.0245559023294
46	22.206030727234754	21.89993499216082	30.52562220738383	25.368412073220593
47	19.073358846213807	23.04230804302872	33.882321103427174	24.002012007330293
48	19.840804332287128	24.53042866933954	32.0396871387432	23.589079859630132
49	23.139437402745624	21.402580899461405	31.86301877579355	23.594962921999418
50	20.9364658679429	21.896522815986632	30.09545268694166	27.071558629128806
51	19.820743089607866	22.15484808462197	28.78047058615892	29.243938239611246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	880.0
2	1507.0
3	5943.5
4	10380.0
5	6755.5
6	3131.0
7	3041.0
8	2951.0
9	3061.0
10	3171.0
11	3211.0
12	3251.0
13	3178.5
14	3106.0
15	3223.0
16	3340.0
17	3361.0
18	3382.0
19	3521.5
20	3661.0
21	4279.0
22	4897.0
23	5822.0
24	6747.0
25	8303.5
26	11832.5
27	13805.0
28	16240.5
29	18676.0
30	21783.5
31	24891.0
32	30172.0
33	35453.0
34	39457.0
35	43461.0
36	47872.0
37	52283.0
38	57332.5
39	62382.0
40	68956.0
41	75530.0
42	83640.0
43	91750.0
44	102915.5
45	114081.0
46	135127.5
47	156174.0
48	171215.0
49	186256.0
50	181955.5
51	177655.0
52	157589.0
53	137523.0
54	122757.5
55	107992.0
56	99276.0
57	90560.0
58	82508.5
59	74457.0
60	65426.5
61	56396.0
62	48423.0
63	40450.0
64	34865.0
65	29280.0
66	24194.0
67	19108.0
68	15667.0
69	12226.0
70	10475.0
71	8724.0
72	6684.5
73	4645.0
74	3965.0
75	2473.5
76	1662.0
77	1182.5
78	703.0
79	555.5
80	408.0
81	293.5
82	179.0
83	154.5
84	130.0
85	75.5
86	21.0
87	14.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1699795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.67699538486169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63903597471106	25.18903515255858
2	11.373611683493083	7.888053597153973
3	4.22324746311147	4.393485983623358
4	2.0787431279053212	2.883382634107431
5	1.2301979975602422	2.132978514193131
6	0.8464541766631241	1.7611492546586431
7	0.6206707079719374	1.5066096693103197
8	0.5234061304258819	1.4520121575349298
9	0.4305440667878106	1.3436977155282441
>10	5.846278625453259	42.403979603581654
>50	0.12755425415085625	2.9571612008486468
>100	0.05490412971714282	3.421401304594111
>500	0.003171355288198123	0.7647939874066212
>1k	0.0021803067606362097	1.902259224900301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4692	0.2760332863668854	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	3594	0.21143726155212836	Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3174	0.18672839960112836	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	2385	0.14031103750746415	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	2374	0.1396639006468427	No Hit
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	2350	0.13825196567821413	Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	2123	0.12489741409993557	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	2029	0.11936733547280702	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1834	0.10789536385269988	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.941531184642854E-4	0.0	0.0	0.22437999876455691	0.0
2	2.941531184642854E-4	0.0	0.0	0.7390891254533635	0.0
3	2.941531184642854E-4	0.0	0.0	1.0856603296279845	0.0
4	2.941531184642854E-4	0.0	0.0	1.5240661373871556	0.0
5	2.941531184642854E-4	0.0	0.0	2.3686974017455045	0.0
6	2.941531184642854E-4	0.0	0.0	3.016363737980168	0.0
7	2.941531184642854E-4	0.0010589512264714274	0.0	3.7947517200603604	0.0
8	2.941531184642854E-4	0.0010589512264714274	0.0	5.002132610108866	0.0
9	2.941531184642854E-4	0.0010589512264714274	0.0	5.657035113057751	0.0
10	2.941531184642854E-4	0.0010589512264714274	0.0	6.824999485232043	0.0
11	2.941531184642854E-4	0.0010589512264714274	0.0	8.763468535911683	0.0
12	2.941531184642854E-4	0.0010589512264714274	0.0	10.534270309066681	0.0
13	2.941531184642854E-4	0.0010589512264714274	0.0	11.087337002403231	0.0
14	2.941531184642854E-4	0.0010589512264714274	0.0	11.331013445739044	0.0
15	2.941531184642854E-4	0.0010589512264714274	0.0	11.638697607652688	0.0
16	2.941531184642854E-4	0.0010589512264714274	0.0	12.234651825661329	0.0
17	2.941531184642854E-4	0.0010589512264714274	0.0	13.073811842016243	0.0
18	2.941531184642854E-4	0.0010589512264714274	0.0	13.925090966851885	0.0
19	2.941531184642854E-4	0.0010589512264714274	0.0	14.602054953685592	0.0
20	2.941531184642854E-4	0.0010589512264714274	0.0	15.341438232257419	0.0
21	2.941531184642854E-4	0.0010589512264714274	0.0	16.385975955924096	0.0
22	3.5298374215714245E-4	0.0010589512264714274	0.0	17.517994817022053	0.0
23	4.118143658499996E-4	0.0010589512264714274	0.0	18.54188299177254	0.0
24	4.118143658499996E-4	0.0010589512264714274	0.0	19.320094481981652	0.0
25	4.118143658499996E-4	0.0010589512264714274	0.0	20.015943099020763	0.0
26	4.118143658499996E-4	0.0010589512264714274	0.0	20.641547951370608	0.0
27	4.118143658499996E-4	0.0010589512264714274	0.0	21.332925441009063	0.0
28	4.118143658499996E-4	0.0010589512264714274	0.0	21.97941516476987	0.0
29	4.118143658499996E-4	0.0010589512264714274	0.0	22.76268608861657	0.0
30	4.118143658499996E-4	0.0010589512264714274	0.0	23.67426660273739	0.0
31	4.118143658499996E-4	0.0010589512264714274	0.0	24.37976344206213	0.0
32	4.7064498954285666E-4	0.0010589512264714274	0.0	25.04443182854403	0.0
33	4.7064498954285666E-4	0.0010589512264714274	0.0	25.7266317408864	0.0
34	4.7064498954285666E-4	0.0010589512264714274	0.0	26.4506013960507	0.0
35	4.7064498954285666E-4	0.0010589512264714274	0.0	27.261169729290884	0.0
36	4.7064498954285666E-4	0.0010589512264714274	0.0	27.963254392441442	0.0
37	4.7064498954285666E-4	0.0010589512264714274	0.0	28.67428131039331	0.0
38	4.7064498954285666E-4	0.0010589512264714274	0.0	29.515853382319634	0.0
39	4.7064498954285666E-4	0.0010589512264714274	0.0	31.21599957641951	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	100	0.0	45.0	12
CGTAAGC	20	7.0342416E-4	45.0	44
CGTTAGG	450	0.0	43.0	2
TCGTTAG	315	0.0	42.857143	1
TTAGGGA	3675	0.0	41.87755	4
TTTAGGG	6230	0.0	41.857944	3
TAGGGAC	1645	0.0	41.854103	5
CTTAGGG	3000	0.0	40.65	3
TCGAGGG	890	0.0	40.449436	3
GCGTAGG	195	0.0	40.384617	2
TAGGGAA	1620	0.0	40.13889	5
CGTTTAT	955	0.0	40.052357	1
TACGGGT	45	1.9292202E-8	40.000004	4
TAGGGAT	1980	0.0	39.772728	5
CTAGGGG	2745	0.0	39.7541	4
TTTCGCG	385	0.0	39.74026	1
TTAGGGG	5785	0.0	39.671566	4
TAGGGCG	675	0.0	39.666668	5
CTAGGGA	2065	0.0	39.661015	4
GCTAGGG	1805	0.0	39.63989	3
>>END_MODULE