SRR2934231.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934231.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	3458	0.5235174071808785	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	3119	0.4721951396752921	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2311	0.3498694991310035	No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2214	0.33518436654090944	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2091	0.31656301284419225	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.13913027680717968	No Hit
CCTGTATCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	912	0.13807052497078115	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCACTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	907	0.1373135593733536	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	758	0.11475598457001326	No Hit
TCTGTATCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	714	0.10809468731265101	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	705	0.10673214923728146	No Hit
GCTGTATCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	688	0.10415846620602787	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACACGT	55	1.8189894E-12	45.000004	41
TCGTTTA	20	7.031106E-4	45.0	11
AATCCCG	20	7.031106E-4	45.0	32
GGTACGC	25	3.888997E-5	45.0	8
CGGCAAA	25	3.888997E-5	45.0	19
CTCCCGT	20	7.031106E-4	45.0	13
GTTACGA	20	7.031106E-4	45.0	18
ACGATAA	20	7.031106E-4	45.0	11
CCCCGAA	20	7.031106E-4	45.0	17
TCGCTTT	35	1.210883E-7	45.0	24
TGCGACC	20	7.031106E-4	45.0	17
TTGCGAG	20	7.031106E-4	45.0	1
GTCCGCG	25	3.888997E-5	45.0	1
GCGATCG	20	7.031106E-4	45.0	9
TGCTCGA	20	7.031106E-4	45.0	42
TAGATCG	40	6.8066583E-9	45.0	36
TTACGAG	25	3.888997E-5	45.0	19
CACCGTA	20	7.031106E-4	45.0	28
CTTAGGT	20	7.031106E-4	45.0	3
GGTTAAC	20	7.031106E-4	45.0	8