##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934231.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30223970980967	33.0	31.0	34.0	31.0	34.0
2	32.474962605899485	34.0	31.0	34.0	31.0	34.0
3	32.519864291207696	34.0	31.0	34.0	31.0	34.0
4	36.00584680227453	37.0	35.0	37.0	35.0	37.0
5	35.13561038677914	37.0	35.0	37.0	35.0	37.0
6	26.075116724095125	36.0	0.0	37.0	0.0	37.0
7	31.087571836035195	37.0	19.0	37.0	17.0	37.0
8	34.72121108439864	37.0	32.0	37.0	32.0	37.0
9	37.20541018451794	39.0	37.0	39.0	34.0	39.0
10	37.34061786559924	39.0	37.0	39.0	34.0	39.0
11	37.405669975110975	39.0	37.0	39.0	35.0	39.0
12	37.07372390739586	39.0	37.0	39.0	34.0	39.0
13	37.028779832014195	39.0	37.0	39.0	33.0	39.0
14	38.13564066540304	40.0	37.0	41.0	33.0	41.0
15	38.26739355549769	40.0	37.0	41.0	34.0	41.0
16	38.34948647453871	40.0	37.0	41.0	34.0	41.0
17	38.30751576002374	40.0	37.0	41.0	34.0	41.0
18	38.28485675183034	40.0	37.0	41.0	34.0	41.0
19	38.317118625592705	40.0	37.0	41.0	34.0	41.0
20	38.25372427073934	40.0	37.0	41.0	34.0	41.0
21	38.1510994168337	40.0	36.0	41.0	34.0	41.0
22	38.12641476870159	40.0	36.0	41.0	34.0	41.0
23	38.0782717567052	40.0	36.0	41.0	34.0	41.0
24	38.04178601490919	40.0	36.0	41.0	34.0	41.0
25	37.93557768586533	40.0	36.0	41.0	34.0	41.0
26	37.879056578636614	40.0	36.0	41.0	34.0	41.0
27	37.88849745356773	40.0	36.0	41.0	34.0	41.0
28	37.793934283274695	40.0	36.0	41.0	34.0	41.0
29	37.75298093052267	40.0	36.0	41.0	34.0	41.0
30	37.542079717560995	40.0	36.0	41.0	33.0	41.0
31	37.5519672021946	40.0	35.0	41.0	33.0	41.0
32	37.45503927137519	39.0	35.0	41.0	33.0	41.0
33	37.38961170692714	39.0	35.0	41.0	33.0	41.0
34	37.286900558943394	40.0	35.0	41.0	33.0	41.0
35	37.26017664549182	40.0	35.0	41.0	33.0	41.0
36	37.22436157521513	40.0	35.0	41.0	33.0	41.0
37	37.1655180975335	39.0	35.0	41.0	32.0	41.0
38	37.04244306104776	39.0	35.0	41.0	32.0	41.0
39	37.00973306365172	39.0	35.0	41.0	32.0	41.0
40	36.964181901860925	39.0	35.0	41.0	32.0	41.0
41	36.89947042686804	39.0	35.0	41.0	32.0	41.0
42	36.888729085040545	39.0	35.0	41.0	32.0	41.0
43	36.802646957301086	39.0	35.0	41.0	32.0	41.0
44	36.65432409027874	39.0	35.0	41.0	31.0	41.0
45	36.603110825819186	39.0	35.0	41.0	31.0	41.0
46	36.505033821222895	39.0	35.0	40.0	31.0	41.0
47	36.435659438149855	39.0	35.0	40.0	31.0	41.0
48	36.44131245723145	39.0	35.0	40.0	31.0	41.0
49	36.427022460683204	39.0	35.0	40.0	31.0	41.0
50	36.32277164467429	38.0	35.0	40.0	31.0	41.0
51	35.29820962496896	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	11.0
11	8.0
12	6.0
13	4.0
14	6.0
15	12.0
16	30.0
17	51.0
18	97.0
19	197.0
20	339.0
21	547.0
22	947.0
23	1366.0
24	2148.0
25	3261.0
26	4538.0
27	5461.0
28	6232.0
29	7414.0
30	9654.0
31	12781.0
32	17508.0
33	30605.0
34	46423.0
35	59396.0
36	60705.0
37	100878.0
38	142579.0
39	147259.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.66981160640211	4.697728497635239	52.560511829858356	18.071948066104294
2	21.618180496932776	4.510606601951155	52.04971144471426	21.82150145640181
3	21.83603519587242	5.194146536428212	50.83372190900668	22.13609635869269
4	20.59082678810413	5.052896755948236	49.66481563345909	24.69146082248854
5	18.382152567930092	8.061683612603174	47.486117250943174	26.07004656852355
6	16.263405860730444	31.13414641531372	39.18069071596834	13.4217570079875
7	80.94657034027118	2.457867294847184	11.386427909624363	5.209134455257278
8	81.64858023532547	2.8184857054616583	9.634506730938092	5.898427328274784
9	75.1023417487722	5.238201934198495	12.80498143920355	6.854474877825753
10	40.269055851949645	25.971035468380034	19.402390800142914	14.357517879527412
11	31.995270478947273	24.036534187594242	25.31353515045448	18.654660183004
12	30.895399465885077	20.084416803425118	30.11405957622038	18.906124154469428
13	25.63660806743655	23.21129029327875	30.764293024410627	20.38780861487407
14	19.5394015732773	26.78734716864588	31.118401530887223	22.5548497271896
15	17.330727353103256	24.483900855673912	37.753053599220024	20.43231819200281
16	20.948871515687355	24.31116130634095	33.89268044545911	20.847286732512583
17	19.506852052587913	23.746767756898983	30.76187073449886	25.984509456014244
18	20.81700810861548	23.94766642645625	32.703638885019956	22.531686579908317
19	23.691206482047804	25.655986386730696	29.941774206245874	20.711032924975626
20	26.6784955157358	25.756965597427527	29.313644153500512	18.25089473333616
21	24.84467065940787	26.332410844591937	29.12743061653334	19.695487879466857
22	20.94796315697044	25.387869172121864	29.745265937153686	23.918901733754005
23	21.768665257701368	25.922892456383646	28.76469270224607	23.543749583668923
24	23.836998056112346	21.9730459690068	30.100131409227714	24.08982456565314
25	21.24181720189181	23.837452235470803	28.538359988615237	26.38237057402215
26	18.610453392114234	25.50459326724519	30.74536888447494	25.139584456165636
27	20.128623594314885	25.17410208740833	31.379100482641263	23.318173835635516
28	18.425753786341918	26.53391508662714	31.490071639224144	23.550259487806798
29	19.675806773933736	25.473557677750662	31.829797799349613	23.020837748965985
30	21.79485626737236	23.50029975837658	31.965748820647597	22.739095153603458
31	23.011148589318914	26.395541775417392	28.927137519454014	21.666172115809683
32	23.85259154741935	26.284267832595543	27.774581700810863	22.088558919174243
33	23.565096013516378	24.177481181835248	28.149431064656973	24.107991739991398
34	20.876505604573286	23.86409742450025	31.437084047404213	23.82231292352225
35	19.906984067388105	23.674704632023882	30.51615970157388	25.90215159901413
36	20.30090896428939	25.298698624744905	30.279229469579068	24.12116294138664
37	22.183179618852684	26.189647132917106	29.41568311603374	22.21149013219647
38	21.866465212889004	27.256817232170434	26.77750661587932	24.09921093906124
39	21.15552312378507	24.692369181205454	30.67633362198955	23.475774073019927
40	22.364699969115804	21.575336244118375	33.03443285109579	23.025530935670034
41	19.475210890615443	23.102892819727128	30.58867700580744	26.833219283849985
42	22.437822845827302	22.445392501801578	27.768374582911953	27.348410069459163
43	22.176366928475836	22.289760374970477	28.419213603580147	27.114659092973543
44	20.777040325071304	22.959977714932812	29.187836471208055	27.07514548878783
45	21.790011687548823	21.9114289693762	28.429962515063618	27.86859682801136
46	21.491464455923406	23.284564563109736	30.959741541666414	24.26422943930044
47	18.54732246128878	23.054144235252796	33.27106029685163	25.12747300660679
48	19.824172031029534	21.18080577473915	32.8974220779614	26.097600116269916
49	21.408046847086894	20.314534345043086	33.36901164515875	24.908407162711267
50	20.221276183440015	20.86817898300158	31.523378125510952	27.38716670804745
51	18.56019087644505	20.518763663229034	30.023829277007017	30.8972161833189
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	480.5
2	823.0
3	3345.0
4	5867.0
5	3806.5
6	1746.0
7	1670.0
8	1594.0
9	1700.0
10	1806.0
11	1765.5
12	1725.0
13	1672.5
14	1620.0
15	1638.5
16	1657.0
17	1578.5
18	1500.0
19	1558.5
20	1617.0
21	1797.0
22	1977.0
23	2165.0
24	2353.0
25	2917.0
26	4186.0
27	4891.0
28	6097.5
29	7304.0
30	8604.5
31	9905.0
32	10652.5
33	11400.0
34	13575.0
35	15750.0
36	16556.5
37	17363.0
38	19724.0
39	22085.0
40	25888.5
41	29692.0
42	33580.5
43	37469.0
44	42523.0
45	47577.0
46	55342.0
47	63107.0
48	71714.0
49	80321.0
50	76831.5
51	73342.0
52	62211.5
53	51081.0
54	44342.5
55	37604.0
56	33932.5
57	30261.0
58	28329.5
59	26398.0
60	25093.0
61	23788.0
62	20593.5
63	17399.0
64	13636.0
65	9873.0
66	8347.5
67	6822.0
68	5156.5
69	3491.0
70	2872.0
71	2253.0
72	1963.5
73	1674.0
74	1261.0
75	669.0
76	490.0
77	353.5
78	217.0
79	167.0
80	117.0
81	94.0
82	71.0
83	49.5
84	28.0
85	16.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	660532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.93742168904368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.6424218190059	23.29546815028816
2	12.52992335989071	8.5046658559202
3	5.061882266522639	5.153616990578129
4	2.6903398956286915	3.6521279809923466
5	1.6489996134067133	2.7981397622626814
6	1.2218375869778113	2.4879610454873737
7	0.9101986451120447	2.162285666797458
8	0.7674501082425939	2.0836222358984897
9	0.6395560596652099	1.9534395321577265
>10	5.687682876876943	38.50542069634106
>50	0.1410779650347685	3.219499659816333
>100	0.050843032840566314	2.8338312388053475
>500	0.005496544090872034	1.2990854450189955
>1k	0.002290226704530014	2.0508357396357075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	3458	0.5235174071808785	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	3119	0.4721951396752921	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2311	0.3498694991310035	No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2214	0.33518436654090944	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2091	0.31656301284419225	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.13913027680717968	No Hit
CCTGTATCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	912	0.13807052497078115	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCACTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	907	0.1373135593733536	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	758	0.11475598457001326	No Hit
TCTGTATCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	714	0.10809468731265101	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	705	0.10673214923728146	No Hit
GCTGTATCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	688	0.10415846620602787	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5139311948550563E-4	0.0	0.0	0.4983861493462845	0.0
2	1.5139311948550563E-4	0.0	0.0	1.7691799943076187	0.0
3	1.5139311948550563E-4	0.0	0.0	2.4478753489611402	0.0
4	1.5139311948550563E-4	0.0	0.0	3.1619966935742703	0.0
5	1.5139311948550563E-4	0.0	0.0	4.3312057553608305	0.0
6	1.5139311948550563E-4	0.0	0.0	5.017924945347084	0.0
7	1.5139311948550563E-4	0.0	0.0	5.931128242083654	0.0
8	1.5139311948550563E-4	0.0	0.0	7.310925133074552	0.0
9	1.5139311948550563E-4	0.0	0.0	7.95404310464898	0.0
10	1.5139311948550563E-4	0.0	0.0	9.281003796939437	0.0
11	1.5139311948550563E-4	0.0	0.0	11.726608249108294	0.0
12	1.5139311948550563E-4	0.0	0.0	14.015066643251197	0.0
13	1.5139311948550563E-4	0.0	0.0	14.763857012226508	0.0
14	1.5139311948550563E-4	0.0	0.0	15.015472376811418	0.0
15	1.5139311948550563E-4	0.0	0.0	15.428018627409422	0.0
16	1.5139311948550563E-4	0.0	0.0	16.3787674177784	0.0
17	1.5139311948550563E-4	0.0	0.0	17.649864048978703	0.0
18	1.5139311948550563E-4	0.0	0.0	19.032234623000853	0.0
19	1.5139311948550563E-4	0.0	0.0	19.977684654187836	0.0
20	1.5139311948550563E-4	0.0	0.0	21.04303803600734	0.0
21	1.5139311948550563E-4	0.0	0.0	22.351225981481594	0.0
22	1.5139311948550563E-4	0.0	0.0	23.742680142672874	0.0
23	1.5139311948550563E-4	0.0	0.0	25.00272507615074	0.0
24	1.5139311948550563E-4	0.0	0.0	25.978150944995853	0.0
25	3.0278623897101126E-4	0.0	0.0	26.85199203066619	0.0
26	3.0278623897101126E-4	0.0	0.0	27.601539365238928	0.0
27	3.0278623897101126E-4	0.0	0.0	28.32610683509656	0.0
28	3.0278623897101126E-4	0.0	0.0	29.064299685707883	0.0
29	3.0278623897101126E-4	0.0	0.0	29.794317307866994	0.0
30	3.0278623897101126E-4	0.0	0.0	30.659831771965628	0.0
31	3.0278623897101126E-4	0.0	0.0	31.467665457540285	0.0
32	3.0278623897101126E-4	0.0	0.0	32.266264162826324	0.0
33	3.0278623897101126E-4	0.0	0.0	33.05699042589912	0.0
34	3.0278623897101126E-4	0.0	0.0	33.80456964991855	0.0
35	3.0278623897101126E-4	0.0	0.0	34.583638642790966	0.0
36	3.0278623897101126E-4	0.0	0.0	35.33379154984164	0.0
37	3.0278623897101126E-4	0.0	0.0	36.099689341318815	0.0
38	3.0278623897101126E-4	0.0	0.0	36.85832026306068	0.0
39	3.0278623897101126E-4	0.0	0.0	37.671906887175794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACACGT	55	1.8189894E-12	45.000004	41
TCGTTTA	20	7.031106E-4	45.0	11
AATCCCG	20	7.031106E-4	45.0	32
GGTACGC	25	3.888997E-5	45.0	8
CGGCAAA	25	3.888997E-5	45.0	19
CTCCCGT	20	7.031106E-4	45.0	13
GTTACGA	20	7.031106E-4	45.0	18
ACGATAA	20	7.031106E-4	45.0	11
CCCCGAA	20	7.031106E-4	45.0	17
TCGCTTT	35	1.210883E-7	45.0	24
TGCGACC	20	7.031106E-4	45.0	17
TTGCGAG	20	7.031106E-4	45.0	1
GTCCGCG	25	3.888997E-5	45.0	1
GCGATCG	20	7.031106E-4	45.0	9
TGCTCGA	20	7.031106E-4	45.0	42
TAGATCG	40	6.8066583E-9	45.0	36
TTACGAG	25	3.888997E-5	45.0	19
CACCGTA	20	7.031106E-4	45.0	28
CTTAGGT	20	7.031106E-4	45.0	3
GGTTAAC	20	7.031106E-4	45.0	8
>>END_MODULE