SRR2934230.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633958
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	9096	1.4347953649926335	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	8211	1.2951962117364242	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	5800	0.9148871060858922	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2803	0.4421428548894406	RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	2684	0.4233718952990576	RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2430	0.38330614961874443	RNA PCR Primer, Index 16 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	2200	0.34702614368775214	No Hit
GAATCAGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	2192	0.34576423043797855	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2126	0.33535344612734597	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	1939	0.30585622391388706	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1612	0.25427551982938934	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	1576	0.24859691020540794	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	1535	0.24212960480031798	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1385	0.21846873136706219	RNA PCR Primer, Index 14 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1020	0.16089393934613966	RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	854	0.13470923941333654	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	816	0.1287151514769117	No Hit
CCTGTATCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	690	0.10884001779297682	RNA PCR Primer, Index 16 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	652	0.10284592985655203	RNA PCR Primer, Index 14 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGG	75	0.0	45.000004	2
GCGCGAC	45	3.8380676E-10	45.000004	9
CGATTCA	30	2.1639553E-6	45.000004	10
ATCCGGC	45	3.8380676E-10	45.000004	6
ATCGACG	30	2.1639553E-6	45.000004	16
GGAATCG	20	7.03089E-4	45.0	8
AGATCGA	20	7.03089E-4	45.0	44
GGTCGTC	20	7.03089E-4	45.0	9
GAATCGA	20	7.03089E-4	45.0	9
TTCGTAG	70	0.0	45.0	1
CGATTTA	35	1.2107739E-7	45.0	10
TAGCCGG	25	3.8888196E-5	45.0	2
TAGTTAG	25	3.8888196E-5	45.0	1
CCCGTTG	20	7.03089E-4	45.0	32
CCGTATG	20	7.03089E-4	45.0	17
ACGATAG	20	7.03089E-4	45.0	40
ACTAGCG	20	7.03089E-4	45.0	15
TAACCGG	20	7.03089E-4	45.0	11
TCTACCG	35	1.2107739E-7	45.0	14
TACGATA	20	7.03089E-4	45.0	39

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content