##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633958
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.287086841715066	33.0	31.0	34.0	31.0	34.0
2	32.436899605336635	34.0	31.0	34.0	30.0	34.0
3	32.5063458462548	34.0	31.0	34.0	30.0	34.0
4	36.02134368522836	37.0	35.0	37.0	35.0	37.0
5	35.14806343637907	37.0	35.0	37.0	35.0	37.0
6	26.016688802728257	36.0	0.0	37.0	0.0	37.0
7	31.04214159297619	37.0	19.0	37.0	17.0	37.0
8	34.5391019594358	37.0	32.0	37.0	32.0	37.0
9	37.16354080238754	39.0	37.0	39.0	34.0	39.0
10	37.3241792043006	39.0	37.0	39.0	34.0	39.0
11	37.37321242101212	39.0	37.0	39.0	35.0	39.0
12	37.09971480760555	39.0	37.0	39.0	34.0	39.0
13	37.044596014247	39.0	37.0	39.0	33.0	39.0
14	38.225357200319266	40.0	37.0	41.0	33.0	41.0
15	38.30388606185268	40.0	37.0	41.0	34.0	41.0
16	38.449408320425015	40.0	37.0	41.0	34.0	41.0
17	38.394415718391436	40.0	37.0	41.0	34.0	41.0
18	38.37199782950921	40.0	37.0	41.0	34.0	41.0
19	38.381533161502816	40.0	37.0	41.0	34.0	41.0
20	38.26781742639102	40.0	37.0	41.0	34.0	41.0
21	38.2224374485376	40.0	37.0	41.0	34.0	41.0
22	38.21881891229388	40.0	37.0	41.0	34.0	41.0
23	38.163786875471246	40.0	36.0	41.0	34.0	41.0
24	38.088303957044474	40.0	36.0	41.0	34.0	41.0
25	37.98050974985725	40.0	36.0	41.0	34.0	41.0
26	37.95429823426789	40.0	36.0	41.0	34.0	41.0
27	37.90510412361702	40.0	36.0	41.0	34.0	41.0
28	37.82457512958272	40.0	36.0	41.0	33.0	41.0
29	37.828179469302384	40.0	36.0	41.0	34.0	41.0
30	37.574323535628544	40.0	36.0	41.0	33.0	41.0
31	37.54671287372349	40.0	36.0	41.0	33.0	41.0
32	37.477924405086775	40.0	35.0	41.0	33.0	41.0
33	37.38566119522113	40.0	35.0	41.0	33.0	41.0
34	37.234129074796755	40.0	35.0	41.0	32.0	41.0
35	37.29689190766581	40.0	35.0	41.0	33.0	41.0
36	37.205220219636004	40.0	35.0	41.0	33.0	41.0
37	37.13692705194981	39.0	35.0	41.0	32.0	41.0
38	37.03069130762606	39.0	35.0	41.0	32.0	41.0
39	36.96744106076428	39.0	35.0	41.0	32.0	41.0
40	36.84362213269649	39.0	35.0	41.0	31.0	41.0
41	36.63834670435581	39.0	35.0	41.0	31.0	41.0
42	36.73518277236031	39.0	35.0	41.0	31.0	41.0
43	36.735184349751876	39.0	35.0	41.0	31.0	41.0
44	36.659196666025196	39.0	35.0	41.0	31.0	41.0
45	36.6021676514848	39.0	35.0	41.0	31.0	41.0
46	36.512062313276274	39.0	35.0	40.0	31.0	41.0
47	36.38134229712379	39.0	35.0	40.0	31.0	41.0
48	36.3785613557996	39.0	35.0	40.0	31.0	41.0
49	36.42300909523975	39.0	35.0	40.0	31.0	41.0
50	36.32101337943523	39.0	35.0	40.0	31.0	41.0
51	35.3142179765852	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	4.0
11	5.0
12	4.0
13	3.0
14	2.0
15	7.0
16	17.0
17	45.0
18	70.0
19	165.0
20	371.0
21	604.0
22	939.0
23	1453.0
24	2168.0
25	3253.0
26	4401.0
27	5377.0
28	6065.0
29	7265.0
30	9278.0
31	12345.0
32	17349.0
33	28200.0
34	42310.0
35	56429.0
36	59814.0
37	98283.0
38	136702.0
39	140940.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.89465863669202	3.6623877291555593	49.52741348795977	17.915540146192647
2	18.501067894087623	11.548241366147284	49.41273712138659	20.537953618378506
3	18.27360803081592	11.334662548623095	49.400433467201296	20.991295953359685
4	17.326699876017024	4.088283450954164	55.34783061338449	23.23718605964433
5	22.140583445591034	7.582205761264942	46.501345515002576	23.775865278141453
6	15.124345776849571	34.74047176626843	37.14946416008632	12.985718296795687
7	77.90090195249527	2.081683644657848	15.014717063275484	5.002697339571391
8	77.45765492351228	6.984847576653343	9.62697844336691	5.930519056467463
9	72.7899955517558	4.247126781269421	11.53215197221267	11.430725694762113
10	42.25327229879582	21.76831903690781	19.62290877313639	16.355499891159983
11	30.76923076923077	24.165954211477732	25.842879181270682	19.221935838020816
12	28.02456945097309	20.594739714618317	30.13306875218863	21.24762208221996
13	23.601248032204026	23.630429776105043	33.64497332630868	19.12334886538225
14	19.42478839292193	28.052016064155673	29.573094747601576	22.95010079532083
15	16.288303010609535	24.492316525700442	38.887591922493286	20.331788541196737
16	17.91932588594197	25.006230696670755	31.908422955463926	25.166020461923345
17	17.955921370185408	24.25555005221166	31.691689354815306	26.096839222787633
18	18.717486016423802	24.598474977837647	32.403250688531415	24.280788317207133
19	21.53581152063701	25.879474665514117	29.915704194915122	22.669009618933746
20	24.038343234094373	25.393480325195046	31.77276728111326	18.795409159597323
21	22.85151382268226	27.565233028055484	29.677675808176566	19.905577341085685
22	19.911729168178333	24.116108638111672	29.9286388057253	26.043523387984692
23	20.09013215386508	26.922445966452035	29.24704791169131	23.74037396799157
24	21.808542521744343	23.33877007625111	29.724839815886856	25.127847586117692
25	18.95393701160014	28.023623016035764	28.32222323876345	24.700216733600648
26	18.888317522611906	25.4302335485947	30.688310582089034	24.993138346704356
27	20.153070077197544	27.574539638272565	28.703478779351315	23.568911505178576
28	16.287987532297095	26.571003126390075	33.485814517680986	23.65519482363185
29	18.685307228554574	22.707813451364288	31.87892573325047	26.727953586830672
30	18.77017089460185	26.613434959413716	29.26834269778124	25.348051448203197
31	20.4150748156818	29.225595386445157	25.37581353969821	24.98351625817483
32	20.71509469081548	29.33506636086302	24.878304240974956	25.071534707346544
33	19.080443814889943	26.051410345795777	24.90133415778332	29.966811681530952
34	18.78673350600513	27.99097101069787	28.18877591259989	25.03351957069711
35	17.061224876095892	25.58907687890996	27.025134157152365	30.324564087841782
36	18.986431277781808	26.631417223222993	27.673599828379796	26.708551670615403
37	18.04867199404377	27.46080970663672	28.181835389726135	26.30868290959338
38	17.495953990642914	28.79559844658479	25.33385492414324	28.37459263862906
39	19.547351717306192	25.590023313847286	27.703885746374368	27.15873922247215
40	19.261686105388684	25.90250458232249	28.32206549960723	26.513743812681597
41	16.851431798321023	28.307238018922387	26.781427160789832	28.059903021966754
42	18.61763713053546	25.99856772846151	28.798280012240557	26.58551512876247
43	20.451828039081455	22.8125522510955	27.536366762466912	29.199252947356136
44	19.583947201549627	23.46559235785336	28.43784604027396	28.51261440032305
45	18.89793961114143	23.543673240183104	27.14959035141129	30.40879679726417
46	21.49826960145625	25.69633950514072	27.826922288227294	24.97846860517574
47	15.84568693825143	24.642326463267285	33.219077604510076	26.29290899397121
48	18.199155149079278	24.816470491736045	28.74370226418785	28.240672094996828
49	18.500594676618956	21.794030519371947	33.1894226431404	26.5159521608687
50	19.44813378804274	22.429719318945416	28.660100511390347	29.462046381621494
51	17.25776786474814	23.589417595487397	26.58362225888781	32.56919228087665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	376.5
2	674.0
3	2825.5
4	4977.0
5	3351.5
6	1726.0
7	1686.0
8	1646.0
9	1625.5
10	1605.0
11	1639.0
12	1673.0
13	1613.0
14	1553.0
15	1434.5
16	1316.0
17	1293.0
18	1270.0
19	1363.0
20	1456.0
21	1591.0
22	1726.0
23	2317.5
24	2909.0
25	3177.0
26	4486.0
27	5527.0
28	6219.5
29	6912.0
30	8021.5
31	9131.0
32	10674.5
33	12218.0
34	14094.0
35	15970.0
36	17185.0
37	18400.0
38	20467.0
39	22534.0
40	26047.0
41	29560.0
42	32934.5
43	36309.0
44	41341.5
45	46374.0
46	67611.0
47	88848.0
48	81078.5
49	73309.0
50	68286.0
51	63263.0
52	53105.0
53	42947.0
54	37478.5
55	32010.0
56	28497.5
57	24985.0
58	23504.0
59	22023.0
60	20087.0
61	18151.0
62	16157.0
63	14163.0
64	11906.0
65	9649.0
66	7804.5
67	5960.0
68	4789.0
69	3618.0
70	3152.5
71	2687.0
72	2218.5
73	1750.0
74	1217.5
75	531.0
76	377.0
77	356.0
78	335.0
79	241.5
80	148.0
81	93.5
82	39.0
83	29.0
84	19.0
85	10.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	633958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.08648904725596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.56407772002686	22.6415350689336
2	11.673057130657954	7.490948395416989
3	4.494649934725943	4.326526077055009
4	2.2772282970189455	2.922730432415989
5	1.4564457830411863	2.3366115832736574
6	1.0598124860429057	2.0403397035336477
7	0.8453729273682683	1.898753442239405
8	0.7299435801072802	1.8737061350581636
9	0.6030698389758042	1.7415354404724799
>10	6.117909865438824	39.34351821062464
>50	0.12267230015987861	2.551648931489492
>100	0.04460810914893425	2.4758541582772886
>500	0.0035483723186652237	0.7753512529567623
>1k	0.00608292397485467	3.8225939053890974
>5k	0.0015207309937136674	3.758347262863836
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	9096	1.4347953649926335	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	8211	1.2951962117364242	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	5800	0.9148871060858922	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2803	0.4421428548894406	RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	2684	0.4233718952990576	RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2430	0.38330614961874443	RNA PCR Primer, Index 16 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	2200	0.34702614368775214	No Hit
GAATCAGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	2192	0.34576423043797855	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2126	0.33535344612734597	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	1939	0.30585622391388706	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1612	0.25427551982938934	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	1576	0.24859691020540794	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	1535	0.24212960480031798	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1385	0.21846873136706219	RNA PCR Primer, Index 14 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1020	0.16089393934613966	RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	854	0.13470923941333654	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	816	0.1287151514769117	No Hit
CCTGTATCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	690	0.10884001779297682	RNA PCR Primer, Index 16 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	652	0.10284592985655203	RNA PCR Primer, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44924111691941737	0.0
2	0.0	0.0	0.0	1.6367014849564168	0.0
3	0.0	0.0	0.0	2.1674937456424557	0.0
4	0.0	0.0	0.0	2.9647074411869556	0.0
5	0.0	0.0	0.0	4.827922354477741	0.0
6	0.0	0.0	0.0	5.379220705472602	0.0
7	0.0	0.0	0.0	6.447745749718435	0.0
8	1.5773915622170555E-4	0.0	0.0	7.84373728228053	0.0
9	1.5773915622170555E-4	0.0	0.0	8.354181191813968	0.0
10	1.5773915622170555E-4	0.0	0.0	10.772006978380272	0.0
11	1.5773915622170555E-4	0.0	0.0	12.710147990876367	0.0
12	1.5773915622170555E-4	0.0	0.0	16.02219705406352	0.0
13	1.5773915622170555E-4	0.0	0.0	16.6717668993845	0.0
14	1.5773915622170555E-4	0.0	0.0	16.95348903239647	0.0
15	1.5773915622170555E-4	0.0	0.0	17.540751911009878	0.0
16	1.5773915622170555E-4	0.0	0.0	18.34948056495856	0.0
17	1.5773915622170555E-4	0.0	0.0	19.3555409033406	0.0
18	1.5773915622170555E-4	0.0	0.0	20.381003157937908	0.0
19	1.5773915622170555E-4	0.0	0.0	21.46609081358702	0.0
20	1.5773915622170555E-4	0.0	0.0	22.28365286028412	0.0
21	1.5773915622170555E-4	0.0	0.0	23.36038034065348	0.0
22	3.154783124434111E-4	0.0	0.0	24.510298789509715	0.0
23	3.154783124434111E-4	0.0	0.0	25.49474886348938	0.0
24	3.154783124434111E-4	0.0	0.0	26.34101943661883	0.0
25	3.154783124434111E-4	0.0	0.0	27.060152249833585	0.0
26	3.154783124434111E-4	0.0	0.0	27.71555844393477	0.0
27	3.154783124434111E-4	0.0	0.0	28.32585123935655	0.0
28	3.154783124434111E-4	0.0	0.0	28.97131986661577	0.0
29	3.154783124434111E-4	0.0	0.0	29.631458235403606	0.0
30	3.154783124434111E-4	0.0	0.0	30.37803766180094	0.0
31	3.154783124434111E-4	0.0	0.0	31.106950302701442	0.0
32	3.154783124434111E-4	0.0	0.0	31.745478407086903	0.0
33	3.154783124434111E-4	0.0	0.0	32.40482808009364	0.0
34	3.154783124434111E-4	0.0	0.0	33.033576356793354	0.0
35	3.154783124434111E-4	0.0	0.0	33.71674464238956	0.0
36	3.154783124434111E-4	0.0	0.0	34.3994397105171	0.0
37	3.154783124434111E-4	0.0	0.0	35.02203616012417	0.0
38	4.732174686651166E-4	0.0	0.0	35.662299395228075	0.0
39	4.732174686651166E-4	0.0	0.0	36.33221759170166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGG	75	0.0	45.000004	2
GCGCGAC	45	3.8380676E-10	45.000004	9
CGATTCA	30	2.1639553E-6	45.000004	10
ATCCGGC	45	3.8380676E-10	45.000004	6
ATCGACG	30	2.1639553E-6	45.000004	16
GGAATCG	20	7.03089E-4	45.0	8
AGATCGA	20	7.03089E-4	45.0	44
GGTCGTC	20	7.03089E-4	45.0	9
GAATCGA	20	7.03089E-4	45.0	9
TTCGTAG	70	0.0	45.0	1
CGATTTA	35	1.2107739E-7	45.0	10
TAGCCGG	25	3.8888196E-5	45.0	2
TAGTTAG	25	3.8888196E-5	45.0	1
CCCGTTG	20	7.03089E-4	45.0	32
CCGTATG	20	7.03089E-4	45.0	17
ACGATAG	20	7.03089E-4	45.0	40
ACTAGCG	20	7.03089E-4	45.0	15
TAACCGG	20	7.03089E-4	45.0	11
TCTACCG	35	1.2107739E-7	45.0	14
TACGATA	20	7.03089E-4	45.0	39
>>END_MODULE