##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934229.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1128005
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.305558929260066	33.0	31.0	34.0	31.0	34.0
2	32.47477892385229	34.0	31.0	34.0	31.0	34.0
3	32.4804916644873	34.0	31.0	34.0	30.0	34.0
4	36.00805226927186	37.0	35.0	37.0	35.0	37.0
5	35.085880824996345	37.0	35.0	37.0	35.0	37.0
6	25.71515551792767	36.0	0.0	37.0	0.0	37.0
7	30.90851015731313	37.0	19.0	37.0	17.0	37.0
8	34.72650032579643	37.0	32.0	37.0	32.0	37.0
9	37.30016090354209	39.0	37.0	39.0	35.0	39.0
10	37.2919596987602	39.0	37.0	39.0	34.0	39.0
11	37.34996919339896	39.0	37.0	39.0	34.0	39.0
12	37.24862833054818	39.0	37.0	39.0	34.0	39.0
13	37.33931853138949	39.0	37.0	39.0	34.0	39.0
14	38.58106480024468	40.0	38.0	41.0	35.0	41.0
15	38.67250588428243	40.0	38.0	41.0	35.0	41.0
16	38.71668033386377	40.0	38.0	41.0	35.0	41.0
17	38.690530627080555	40.0	38.0	41.0	35.0	41.0
18	38.64166559545392	40.0	38.0	41.0	35.0	41.0
19	38.61370472648614	40.0	38.0	41.0	35.0	41.0
20	38.508376292658276	40.0	38.0	41.0	34.0	41.0
21	38.39768440742727	40.0	37.0	41.0	34.0	41.0
22	38.38001870559084	40.0	37.0	41.0	34.0	41.0
23	38.300527036670935	40.0	37.0	41.0	34.0	41.0
24	38.25285792172907	40.0	37.0	41.0	34.0	41.0
25	38.12852513951623	40.0	37.0	41.0	34.0	41.0
26	38.084527107592606	40.0	37.0	41.0	34.0	41.0
27	38.06011764132251	40.0	37.0	41.0	34.0	41.0
28	37.96824659465162	40.0	37.0	41.0	34.0	41.0
29	37.923553530347824	40.0	36.0	41.0	34.0	41.0
30	37.676253208097485	40.0	36.0	41.0	33.0	41.0
31	37.6760971804203	40.0	36.0	41.0	33.0	41.0
32	37.52758542736956	40.0	36.0	41.0	33.0	41.0
33	37.33801711871845	40.0	36.0	41.0	33.0	41.0
34	37.18759579966401	40.0	36.0	41.0	32.0	41.0
35	37.102022597417566	40.0	36.0	41.0	32.0	41.0
36	37.023160358331744	40.0	36.0	41.0	31.0	41.0
37	36.95618281833857	40.0	36.0	41.0	31.0	41.0
38	36.8621309302707	40.0	36.0	41.0	31.0	41.0
39	36.800726947132326	40.0	35.0	41.0	31.0	41.0
40	36.77104179502751	40.0	35.0	41.0	31.0	41.0
41	36.699611260588384	40.0	35.0	41.0	31.0	41.0
42	36.62911334612878	39.0	35.0	41.0	31.0	41.0
43	36.55623157698769	39.0	35.0	41.0	30.0	41.0
44	36.44411682572329	39.0	35.0	41.0	30.0	41.0
45	36.416612515015444	39.0	35.0	41.0	30.0	41.0
46	36.334242312755705	39.0	35.0	41.0	30.0	41.0
47	36.27650232046844	39.0	35.0	41.0	30.0	41.0
48	36.224009645347316	39.0	35.0	41.0	30.0	41.0
49	36.19389275756756	39.0	35.0	41.0	30.0	41.0
50	36.12039042380131	39.0	35.0	41.0	29.0	41.0
51	35.223232166524085	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	14.0
11	14.0
12	9.0
13	10.0
14	13.0
15	21.0
16	35.0
17	76.0
18	161.0
19	353.0
20	664.0
21	1248.0
22	2016.0
23	3428.0
24	5992.0
25	9569.0
26	12440.0
27	12774.0
28	12340.0
29	12983.0
30	15272.0
31	19456.0
32	26394.0
33	40116.0
34	60665.0
35	84233.0
36	104544.0
37	177626.0
38	253044.0
39	272340.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.613547812288065	4.165761676588313	56.33662971352077	15.884060797602848
2	22.840501593521306	4.493774407028337	51.90748267959805	20.758241319852306
3	19.013036289732757	4.493774407028337	55.06278784225247	21.43040146098643
4	16.837336713933006	4.1710808019467995	55.15285836498952	23.838724119130678
5	16.36579625090314	7.853777243895195	53.31705089959707	22.463375605604586
6	14.5037477670755	31.858014813764125	41.379603813812885	12.258633605347494
7	82.86683126404581	1.4617843006015045	11.793564744837123	3.8778196905155564
8	85.36318544687302	1.834477684052819	9.226377542652736	3.5759593264214256
9	80.49459000624998	4.208669287813441	10.975394612612533	4.321346093324054
10	36.71765639336705	28.808826202011517	19.661083062575077	14.812434342046357
11	24.53969618928994	26.022225078789546	29.214232206417524	20.22384652550299
12	22.390503588193315	22.246532595156935	33.83992092233633	21.523042894313413
13	22.23695816951166	23.757962065770986	34.58867646863267	19.41640329608468
14	20.047340215690536	25.693325827456437	33.876179626863355	20.383154329989672
15	18.198323588991183	25.998643623033583	35.76092304555388	20.042109742421353
16	20.0983151670427	25.79332538419599	33.279551065819746	20.828808382941567
17	20.48714323074809	25.694566956706755	31.051280801060276	22.76700901148488
18	21.04831095606846	24.532426717966676	32.83886152986911	21.580400796095763
19	21.58430148802532	27.839770213784515	30.330627967074612	20.245300331115555
20	22.31869539585374	26.984454856139823	31.20695386988533	19.489895878121107
21	22.181639265783396	27.90351106599705	31.099064277197353	18.815785391022203
22	21.106732682922505	25.249356164201398	31.243921791126812	22.399989361749284
23	20.067464239963474	26.57904885173381	30.1327565037389	23.22073040456381
24	19.968705812474237	25.488450849065387	31.851365907066015	22.691477431394365
25	19.132273349852174	28.271505888715033	29.442599988475227	23.153620772957566
26	18.649651375658795	28.42762221798662	30.118483517360296	22.804242888994285
27	18.941671357839727	27.32310583729682	30.925838094689297	22.809384710174157
28	18.16835918280504	25.7814460042287	32.46058306479138	23.589611748174875
29	17.882810803143602	24.183935354896477	33.18159050713428	24.751663334825643
30	19.627306616548683	25.599886525325687	31.914220238385465	22.85858661974016
31	19.26223731277787	27.69145526837204	28.963524097854176	24.08278332099592
32	18.293535932908096	28.68595440623047	28.90120167907057	24.11930798179086
33	18.95505782332525	26.584102020824375	28.941981640152303	25.518858515698067
34	17.780240335814117	25.72382214617843	30.122384209289855	26.3735533087176
35	17.065704495990712	25.90174688941982	30.423712660848135	26.608835953741337
36	18.020842106196337	25.862030753409783	30.19543353087974	25.92169360951414
37	17.016147978067472	26.89402972504554	29.434798604616113	26.65502369227087
38	17.213576180956643	28.1083860443881	29.11946312294715	25.558574651708106
39	18.5262476673419	27.14854987344914	30.577524035797715	23.747678423411244
40	19.760284750510856	25.067708033209072	30.171054206320008	25.000953009960064
41	17.5889291270872	24.60609660418172	29.582049724956892	28.222924543774187
42	18.22013200296098	24.98082898568712	29.793662262135364	27.005376749216538
43	18.25266731973706	24.986325415224222	31.27610249954566	25.48490476549306
44	18.47846419120483	25.643326049086664	28.90253146041019	26.975678299298316
45	19.134046391638336	24.606185256271026	28.163616296027055	28.096152056063584
46	19.45026839420038	25.31114667044916	28.653685045722316	26.58489988962815
47	16.700014627594737	24.96292126364688	31.050128323899273	27.28693578485911
48	16.643543246705466	25.165579939805234	31.344630564580832	26.84624624890847
49	18.76037783520463	23.67959361882261	31.829468841006907	25.730559704965845
50	18.384847584895457	23.393601978714635	30.752168651734703	27.469381784655212
51	16.91605976923861	23.0567240393438	29.311838156745758	30.71537803467183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	380.5
2	630.0
3	6959.0
4	13288.0
5	8810.5
6	4333.0
7	4429.0
8	4525.0
9	4825.0
10	5125.0
11	5427.0
12	5729.0
13	5734.5
14	5740.0
15	5755.5
16	5771.0
17	5475.0
18	5179.0
19	5160.5
20	5142.0
21	5165.5
22	5189.0
23	5636.5
24	6084.0
25	6737.5
26	8393.5
27	9396.0
28	10765.5
29	12135.0
30	14096.5
31	16058.0
32	19172.5
33	22287.0
34	25942.5
35	29598.0
36	33512.0
37	37426.0
38	41454.0
39	45482.0
40	51824.5
41	58167.0
42	65889.0
43	73611.0
44	82866.0
45	92121.0
46	106502.0
47	120883.0
48	129391.0
49	137899.0
50	129486.5
51	121074.0
52	103025.0
53	84976.0
54	72089.0
55	59202.0
56	50411.5
57	41621.0
58	36616.5
59	31612.0
60	26830.0
61	22048.0
62	18803.0
63	15558.0
64	12355.0
65	9152.0
66	7316.0
67	5480.0
68	4527.0
69	3574.0
70	2737.0
71	1900.0
72	1524.5
73	1149.0
74	893.0
75	523.0
76	409.0
77	273.5
78	138.0
79	125.5
80	113.0
81	67.0
82	21.0
83	16.5
84	12.0
85	9.0
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1128005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.97974475521145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80341214810797	30.982484040412437
2	11.172997065992126	9.380791299621517
3	4.171850838364125	5.253997000540226
4	2.2366243488864255	3.755716771181726
5	1.463437511392156	3.0717366596722284
6	1.0299270357245003	2.594164444572364
7	0.8158572616179862	2.397463571958618
8	0.6498819304357233	2.1825502048572596
9	0.5494110475860663	2.0757721989020754
>10	3.9690605189294508	30.530849606017668
>50	0.09475468027870028	2.6720931147860525
>100	0.03820144010353724	2.5889917711715893
>500	0.003056115054175015	0.9072284329832718
>1k	0.001309763594646435	1.0289253379329473
>5k	2.1829393244107248E-4	0.5772355453900636
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6299	0.5584195105518149	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	2523	0.22366922132437358	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2430	0.21542457701871887	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1773	0.1571801543432875	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1619	0.1435277325898378	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1548	0.13723343424896164	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	1335	0.1183505392263332	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7730417861622954E-4	0.0	0.0	0.23670107845266644	0.0
2	1.7730417861622954E-4	0.0	0.0	0.7103691916259236	0.0
3	1.7730417861622954E-4	0.0	0.0	1.0688782407879398	0.0
4	1.7730417861622954E-4	0.0	0.0	1.5515002149813166	0.0
5	1.7730417861622954E-4	0.0	0.0	2.430928940917815	0.0
6	1.7730417861622954E-4	0.0	0.0	3.2734783977021378	0.0
7	1.7730417861622954E-4	0.0	0.0	4.127729930275132	0.0
8	1.7730417861622954E-4	0.0	0.0	5.4173518734402775	0.0
9	1.7730417861622954E-4	0.0	0.0	6.016374040895209	0.0
10	1.7730417861622954E-4	0.0	0.0	7.141280402125877	0.0
11	1.7730417861622954E-4	0.0	0.0	9.33577422085895	0.0
12	2.659562679243443E-4	0.0	0.0	11.509168842336692	0.0
13	2.659562679243443E-4	0.0	0.0	12.327427626650591	0.0
14	2.659562679243443E-4	0.0	0.0	12.626805732244094	0.0
15	2.659562679243443E-4	0.0	0.0	12.925474621123133	0.0
16	2.659562679243443E-4	0.0	0.0	13.57032991875036	0.0
17	3.5460835723245907E-4	0.0	0.0	14.562612754376088	0.0
18	3.5460835723245907E-4	0.0	0.0	15.651260411079738	0.0
19	3.5460835723245907E-4	0.0	0.0	16.519341669584797	0.0
20	3.5460835723245907E-4	0.0	0.0	17.380419413034517	0.0
21	3.5460835723245907E-4	0.0	0.0	18.67775408796947	0.0
22	3.5460835723245907E-4	0.0	0.0	20.118705147583565	0.0
23	3.5460835723245907E-4	0.0	0.0	21.51949681074109	0.0
24	4.4326044654057384E-4	0.0	0.0	22.53181501855045	0.0
25	4.4326044654057384E-4	0.0	0.0	23.399984929144818	0.0
26	4.4326044654057384E-4	0.0	0.0	24.12161293611287	0.0
27	4.4326044654057384E-4	0.0	0.0	24.84634376620671	0.0
28	4.4326044654057384E-4	0.0	0.0	25.624886414510573	0.0
29	4.4326044654057384E-4	0.0	0.0	26.475414559332627	0.0
30	4.4326044654057384E-4	0.0	0.0	27.408655103479152	0.0
31	4.4326044654057384E-4	0.0	0.0	28.275761189001823	0.0
32	4.4326044654057384E-4	0.0	0.0	29.073807296953472	0.0
33	4.4326044654057384E-4	0.0	0.0	29.886303695462342	0.0
34	4.4326044654057384E-4	0.0	0.0	30.72539572076365	0.0
35	4.4326044654057384E-4	0.0	0.0	31.56767922128005	0.0
36	4.4326044654057384E-4	0.0	0.0	32.35260482001409	0.0
37	4.4326044654057384E-4	0.0	0.0	33.14284954410663	0.0
38	4.4326044654057384E-4	0.0	0.0	33.932739659841936	0.0
39	4.4326044654057384E-4	0.0	0.0	34.78424297764638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAG	30	2.165465E-6	45.000004	1
TATTGCG	20	7.03323E-4	45.000004	1
CCCGTAG	20	7.03323E-4	45.000004	1
GCGATCG	30	2.165465E-6	45.000004	9
TCCCGCA	30	2.165465E-6	45.000004	17
TACGGAC	25	3.89076E-5	45.0	4
CCGTCGG	25	3.89076E-5	45.0	2
CGTTTAT	1660	0.0	44.32229	1
GCTACGA	140	0.0	43.392857	10
CGTTTTT	3735	0.0	43.07229	1
CGGTAGT	115	0.0	43.043476	12
TAGGGCG	570	0.0	42.236843	5
TTAGGGA	2730	0.0	42.115387	4
CCGTAGG	150	0.0	42.0	2
CGAATAT	140	0.0	41.785713	14
TACGGTT	65	0.0	41.53846	33
AGGGCGA	845	0.0	41.53846	6
TCCGTAG	125	0.0	41.399998	1
TTTAGGG	4405	0.0	40.913734	3
CGTTAGG	275	0.0	40.909092	2
>>END_MODULE