SRR2934228.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625564
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2348	0.3753412920180829	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	1967	0.3144362527255405	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1611	0.2575276070873644	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	1544	0.24681727209366267	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	1435	0.2293929957606256	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1389	0.2220396314365916	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.18319468511615117	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	978	0.15633891975880965	No Hit
CCTGTATCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	696	0.11125959933755779	TruSeq Adapter, Index 21 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGC	30	2.163908E-6	45.000004	32
GACGATA	30	2.163908E-6	45.000004	9
GCGCTAG	45	3.8380676E-10	45.000004	1
TATCAGG	25	3.8887607E-5	45.0	2
GTACCGA	20	7.030819E-4	45.0	40
ACCGGGC	20	7.030819E-4	45.0	11
CTCGTCA	25	3.8887607E-5	45.0	45
ACGCACA	20	7.030819E-4	45.0	38
GCGCGAT	20	7.030819E-4	45.0	40
TTCGTCC	25	3.8887607E-5	45.0	16
CACCTAT	25	3.8887607E-5	45.0	10
CCCGTAA	20	7.030819E-4	45.0	16
TAACCGA	20	7.030819E-4	45.0	16
GTTAAAC	20	7.030819E-4	45.0	24
ATATACG	25	3.8887607E-5	45.0	20
CGTACCC	20	7.030819E-4	45.0	38
CATCGCG	25	3.8887607E-5	45.0	1
CTTTCGT	20	7.030819E-4	45.0	15
CGATGAA	20	7.030819E-4	45.0	19
TAGGCAC	20	7.030819E-4	45.0	5