##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625564
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39845163724255	33.0	31.0	34.0	31.0	34.0
2	32.564915819964064	34.0	31.0	34.0	31.0	34.0
3	32.61787443011426	34.0	31.0	34.0	31.0	34.0
4	36.08366530043289	37.0	35.0	37.0	35.0	37.0
5	35.19474745989219	37.0	35.0	37.0	35.0	37.0
6	26.098627798274837	37.0	0.0	37.0	0.0	37.0
7	31.11786324021203	37.0	19.0	37.0	17.0	37.0
8	34.770925117174265	37.0	32.0	37.0	32.0	37.0
9	37.29162803486134	39.0	37.0	39.0	35.0	39.0
10	37.43608487700699	39.0	37.0	39.0	35.0	39.0
11	37.51055687347738	39.0	37.0	39.0	35.0	39.0
12	37.16368908696792	39.0	37.0	39.0	34.0	39.0
13	37.128116387771676	39.0	37.0	39.0	33.0	39.0
14	38.29671464470462	40.0	37.0	41.0	34.0	41.0
15	38.42618181353147	40.0	37.0	41.0	34.0	41.0
16	38.506152847670265	40.0	37.0	41.0	35.0	41.0
17	38.48136561566842	40.0	37.0	41.0	34.0	41.0
18	38.440335441297776	40.0	37.0	41.0	34.0	41.0
19	38.46449763733207	40.0	37.0	41.0	34.0	41.0
20	38.38865088144458	40.0	37.0	41.0	34.0	41.0
21	38.29477879161844	40.0	37.0	41.0	34.0	41.0
22	38.2750398040808	40.0	37.0	41.0	34.0	41.0
23	38.23219846410599	40.0	37.0	41.0	34.0	41.0
24	38.20039036773216	40.0	36.0	41.0	34.0	41.0
25	38.08937854480117	40.0	36.0	41.0	34.0	41.0
26	38.034244297945534	40.0	36.0	41.0	34.0	41.0
27	38.01480104353831	40.0	36.0	41.0	34.0	41.0
28	37.939181282810395	40.0	36.0	41.0	34.0	41.0
29	37.918598256932945	40.0	36.0	41.0	34.0	41.0
30	37.667352980670245	40.0	36.0	41.0	33.0	41.0
31	37.695263793952336	40.0	36.0	41.0	33.0	41.0
32	37.61203010403412	40.0	35.0	41.0	33.0	41.0
33	37.52077964844524	40.0	35.0	41.0	33.0	41.0
34	37.4578060757972	40.0	35.0	41.0	33.0	41.0
35	37.41571286071449	40.0	35.0	41.0	33.0	41.0
36	37.333681286007504	40.0	35.0	41.0	33.0	41.0
37	37.30237193956174	40.0	35.0	41.0	33.0	41.0
38	37.19617657026299	39.0	35.0	41.0	33.0	41.0
39	37.15361337928653	39.0	35.0	41.0	33.0	41.0
40	37.022454936665156	39.0	35.0	41.0	32.0	41.0
41	36.927743604171596	39.0	35.0	41.0	32.0	41.0
42	36.91461465173827	39.0	35.0	41.0	32.0	41.0
43	36.88440830994111	39.0	35.0	41.0	32.0	41.0
44	36.77304960004092	39.0	35.0	41.0	32.0	41.0
45	36.72219629006784	39.0	35.0	41.0	31.0	41.0
46	36.63624345390719	39.0	35.0	41.0	31.0	41.0
47	36.57059709318311	39.0	35.0	40.0	31.0	41.0
48	36.5275095753592	39.0	35.0	40.0	31.0	41.0
49	36.48303131254356	39.0	35.0	40.0	31.0	41.0
50	36.3754451982531	38.0	35.0	40.0	31.0	41.0
51	35.32888561362227	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	7.0
12	11.0
13	6.0
14	6.0
15	7.0
16	26.0
17	41.0
18	92.0
19	204.0
20	320.0
21	535.0
22	823.0
23	1248.0
24	1937.0
25	2783.0
26	3824.0
27	4747.0
28	5397.0
29	6276.0
30	8269.0
31	11288.0
32	15838.0
33	27392.0
34	42935.0
35	56985.0
36	55900.0
37	94375.0
38	135513.0
39	148674.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.006611633661787	4.438714504031562	52.175636705437014	17.37903715686964
2	20.60156914400445	5.534046076820277	51.35477744883018	22.509607330345098
3	20.726256626020678	5.731467923345972	51.18245295445391	22.359822496179447
4	19.96790096616813	4.392356337640913	51.164229399389995	24.475513296800965
5	19.16062944798614	8.256229578428426	48.04991975241542	24.533221221170017
6	16.568568523764153	32.007756200804394	38.19673126970222	13.22694400572923
7	83.11347839709447	2.2045705954946255	9.936473326470194	4.745477680940719
8	84.08236407465904	3.230045207205018	7.723270520682137	4.964320197453818
9	78.56414371671005	4.939542556796747	10.025672832835648	6.470640893657563
10	40.75809989065867	27.899943091354363	16.768867773720995	14.573089244265974
11	33.10356734083164	25.460384548982994	24.038787398251817	17.39726071193355
12	31.50820699400861	21.171135167624737	28.401090855611894	18.919566982754763
13	26.0336272547653	24.498852235742465	29.519281800103585	19.948238709388647
14	19.749857728385905	28.3070956768612	29.142821517862284	22.800225076890616
15	17.482303968898467	25.93052029848265	36.575794003491254	20.011381729127635
16	20.74927585346983	23.59486799112481	34.68549980497599	20.970356350429373
17	21.208541412229604	22.794470270028327	30.57944510873388	25.41754320900819
18	21.238594292510438	24.517715213791075	30.976207070739363	23.26748342295912
19	24.715616627555296	25.54095184505502	28.286314429858493	21.45711709753119
20	28.65877831844543	24.930622606160203	28.009284421737824	18.401314653656538
21	27.006029758745708	25.69729076481383	26.785908396263213	20.51077108017725
22	22.857613289767315	25.45015378122782	27.31535062759366	24.376882301411207
23	23.42957714958022	26.264778663733846	27.341247258473956	22.96439692821198
24	24.07251696069467	22.633175822138103	29.27054625905583	24.0237609581114
25	22.137942720489033	24.925667078028788	28.056601722605524	24.87978847887666
26	21.405323835770602	27.512133051134658	27.99857408674412	23.08396902635062
27	22.444705897398187	26.995958846736706	28.434180995070047	22.125154260795057
28	19.40472917239483	26.730758163832956	30.79972632696255	23.06478633680966
29	22.187178290310825	24.8044964224284	30.175809349642886	22.832515937617895
30	24.096495322620868	24.14301334475769	29.14633834427812	22.61415298834332
31	24.552723622203324	25.670914566695014	25.81958041063744	23.956781400464223
32	25.50610329238895	26.145366421341382	27.47312824906804	20.875402037201628
33	24.97698077255085	24.77764065707106	28.18176877186027	22.063609798517817
34	22.809496710168744	24.518034925283423	29.347756584458185	23.324711780089647
35	22.67841499830553	22.541418623833852	30.167656706587977	24.612509671272644
36	22.81077555613814	25.240742753739028	29.80206661508655	22.146415075036288
37	24.569828187044013	25.73293859621078	28.806165316418465	20.891067900326746
38	22.71662052164127	24.838066129125078	27.898184678146443	24.54712867108721
39	24.1391768068495	23.472738201047374	29.827483678728317	22.56060131337481
40	24.526986847069203	22.00670115287964	31.63785000415625	21.828461995894905
41	20.63386000473173	23.490642044618937	31.330127692770045	24.54537025787929
42	22.3582239387177	23.491601179095984	29.918441598301694	24.231733283884623
43	23.34916970925437	23.35236682417786	29.164561899342033	24.133901567225735
44	22.951288757025658	24.12367079947056	28.235480302574956	24.689560140928826
45	22.605360922303714	22.93754116285464	27.31678932930923	27.140308585532413
46	22.97222985977454	24.68364547832036	29.093106380801963	23.251018281103132
47	20.922079915084627	23.581599964192314	31.593090395227346	23.903229725495713
48	21.753969218177517	21.491166371466388	31.694758649794423	25.06010576056167
49	21.761002871009204	21.51530458913876	33.16766949504767	23.55602304480437
50	22.16543790883107	21.580206022085672	30.677596536885115	25.576759532198146
51	19.801331278654143	22.553088093304602	29.132910461599455	28.5126701664418
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	457.0
2	796.0
3	2019.5
4	3243.0
5	2208.5
6	1174.0
7	1154.0
8	1134.0
9	1217.0
10	1300.0
11	1314.5
12	1329.0
13	1324.0
14	1319.0
15	1379.0
16	1439.0
17	1361.0
18	1283.0
19	1240.5
20	1198.0
21	1453.5
22	1709.0
23	2105.5
24	2502.0
25	3004.0
26	4276.5
27	5047.0
28	5763.0
29	6479.0
30	7666.5
31	8854.0
32	10237.0
33	11620.0
34	13021.5
35	14423.0
36	16129.5
37	17836.0
38	20612.0
39	23388.0
40	26764.5
41	30141.0
42	33906.0
43	37671.0
44	40504.0
45	43337.0
46	50641.0
47	57945.0
48	61699.5
49	65454.0
50	63054.5
51	60655.0
52	53853.5
53	47052.0
54	41682.0
55	36312.0
56	33580.5
57	30849.0
58	30803.0
59	30757.0
60	28825.5
61	26894.0
62	22269.5
63	17645.0
64	14426.0
65	11207.0
66	9334.0
67	7461.0
68	6374.5
69	5288.0
70	4092.0
71	2896.0
72	2349.0
73	1802.0
74	1461.5
75	896.5
76	672.0
77	509.0
78	346.0
79	251.0
80	156.0
81	123.0
82	90.0
83	65.5
84	41.0
85	50.5
86	60.0
87	34.0
88	8.0
89	6.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	625564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.800479484519084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.30396474451366	21.65395823793746
2	11.48266852093511	7.073433924131899
3	4.287736886742634	3.961930560453967
4	2.1881988428343173	2.695902942670672
5	1.398410447840557	2.1535856154825113
6	1.0115870928800337	1.8694420500633473
7	0.871843693765144	1.879724266246446
8	0.6886333579123826	1.6968190090188664
9	0.6295164259207555	1.7450466985566009
>10	6.849886744679464	45.631305663070535
>50	0.23336876914091179	4.638076382623063
>100	0.047210233757232455	2.4658525012602053
>500	0.003218879574356758	0.6445949612957221
>1k	0.0037553595034162178	1.890327187188685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2348	0.3753412920180829	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	1967	0.3144362527255405	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1611	0.2575276070873644	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	1544	0.24681727209366267	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	1435	0.2293929957606256	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1389	0.2220396314365916	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.18319468511615117	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	978	0.15633891975880965	No Hit
CCTGTATCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	696	0.11125959933755779	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33249995204327615	0.0
2	0.0	0.0	0.0	1.2681356344035142	0.0
3	0.0	0.0	0.0	1.7641680147834593	0.0
4	0.0	0.0	0.0	2.286256881789873	0.0
5	0.0	0.0	0.0	3.1991930481933104	0.0
6	0.0	0.0	0.0	3.712969416398642	0.0
7	0.0	0.0	0.0	4.373813071084653	0.0
8	0.0	0.0	0.0	5.415433113158685	0.0
9	0.0	0.0	0.0	5.866705884609728	0.0
10	0.0	0.0	0.0	7.01894610303662	0.0
11	0.0	0.0	0.0	8.627894188284492	0.0
12	0.0	0.0	0.0	10.320926396020232	0.0
13	0.0	0.0	0.0	10.855643866974443	0.0
14	0.0	0.0	0.0	11.046831339399326	0.0
15	0.0	0.0	0.0	11.373896196072664	0.0
16	0.0	0.0	0.0	12.052963405822586	0.0
17	0.0	0.0	0.0	12.936166403437538	0.0
18	0.0	0.0	0.0	13.956205919777993	0.0
19	0.0	0.0	0.0	14.656853655261491	0.0
20	0.0	0.0	0.0	15.37428624409333	0.0
21	0.0	0.0	0.0	16.319673126970223	0.0
22	1.59855746174652E-4	0.0	0.0	17.34738571912706	0.0
23	1.59855746174652E-4	0.0	0.0	18.275668037163264	0.0
24	1.59855746174652E-4	0.0	0.0	18.981591012270528	0.0
25	1.59855746174652E-4	0.0	0.0	19.631884187709012	0.0
26	1.59855746174652E-4	0.0	0.0	20.18434564648861	0.0
27	1.59855746174652E-4	0.0	0.0	20.7160258582655	0.0
28	1.59855746174652E-4	0.0	0.0	21.26337193316751	0.0
29	1.59855746174652E-4	0.0	0.0	21.87434059504703	0.0
30	1.59855746174652E-4	0.0	0.0	22.543816460026473	0.0
31	3.19711492349304E-4	0.0	0.0	23.21137405605182	0.0
32	3.19711492349304E-4	0.0	0.0	23.83273334143269	0.0
33	3.19711492349304E-4	0.0	0.0	24.431552966602936	0.0
34	4.79567238523956E-4	0.0	0.0	25.05946633757697	0.0
35	4.79567238523956E-4	0.0	0.0	25.69217538093624	0.0
36	6.39422984698608E-4	0.0	0.0	26.337193316750962	0.0
37	6.39422984698608E-4	0.0	0.0	26.97309947503373	0.0
38	6.39422984698608E-4	0.0	0.0	27.552256843424495	0.0
39	6.39422984698608E-4	0.0	0.0	28.19056083789988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGC	30	2.163908E-6	45.000004	32
GACGATA	30	2.163908E-6	45.000004	9
GCGCTAG	45	3.8380676E-10	45.000004	1
TATCAGG	25	3.8887607E-5	45.0	2
GTACCGA	20	7.030819E-4	45.0	40
ACCGGGC	20	7.030819E-4	45.0	11
CTCGTCA	25	3.8887607E-5	45.0	45
ACGCACA	20	7.030819E-4	45.0	38
GCGCGAT	20	7.030819E-4	45.0	40
TTCGTCC	25	3.8887607E-5	45.0	16
CACCTAT	25	3.8887607E-5	45.0	10
CCCGTAA	20	7.030819E-4	45.0	16
TAACCGA	20	7.030819E-4	45.0	16
GTTAAAC	20	7.030819E-4	45.0	24
ATATACG	25	3.8887607E-5	45.0	20
CGTACCC	20	7.030819E-4	45.0	38
CATCGCG	25	3.8887607E-5	45.0	1
CTTTCGT	20	7.030819E-4	45.0	15
CGATGAA	20	7.030819E-4	45.0	19
TAGGCAC	20	7.030819E-4	45.0	5
>>END_MODULE