SRR2934227.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934227.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1494433
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	3109	0.20803876788052728	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3075	0.20576365752094605	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	2906	0.19445502073361604	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	2862	0.19151076026827565	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2833	0.18957022496157405	TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	2388	0.15979304525529078	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2163	0.14473716787570937	TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2065	0.13817949683926947	TruSeq Adapter, Index 23 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.033967E-4	45.0	25
GCGATAC	40	6.8175723E-9	45.0	9
TACGTCG	20	7.033967E-4	45.0	43
TAGGGCG	660	0.0	43.977272	5
TCGTTAG	265	0.0	42.45283	1
CGTTTAT	855	0.0	42.36842	1
CGTTAGG	495	0.0	42.272728	2
TCGTAGG	420	0.0	41.785717	2
TAGGGCA	1325	0.0	41.603775	5
CGTTTTT	2335	0.0	41.33833	1
TTTAGGG	5765	0.0	40.98005	3
CGTAGGG	645	0.0	40.813953	3
CTTAGGG	2870	0.0	40.688156	3
TTCGTAG	295	0.0	40.42373	1
CGTAAGG	45	1.9290383E-8	40.000004	2
TGTAGGG	2310	0.0	39.837658	3
TCTTAGG	1615	0.0	39.705883	2
GCGTTAG	160	0.0	39.375	1
TTAGGGG	4930	0.0	39.066936	4
TCTAGGG	2930	0.0	39.01024	3