FastQCFastQC Report
Sat 14 Jan 2017
SRR2934225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246121
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA54100.43414724573295854No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT39460.3166626675900655No Hit
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT29130.23376542085399413TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC25720.20640050203792407TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23980.19243717102913763No Hit
TCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC22510.18064056379757665TruSeq Adapter, Index 22 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGCG19710.1581708357374605No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC19690.15801033767988823TruSeq Adapter, Index 22 (95% over 21bp)
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATG19600.15728809642081307No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTA16250.13040467177745982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGT207.0335146E-445.00000428
ATACTCG351.2120472E-745.00000444
TATTCGC207.0335146E-445.00000425
TTAGTCG302.1656488E-645.00000440
CAATCGT302.1656488E-645.00000438
CGATAAC207.0335146E-445.00000416
CGAGGGT1100.045.04
GTCCGTA253.890995E-545.09
CGTAACA253.890995E-545.014
TCGTTAG1650.043.6363641
CTTGACC20500.043.024397
AACTTGA20650.042.276035
CCTGCAT16600.042.15361413
TAGGGCG5250.042.05
CTCAACT21500.041.965122
GCATTAG15900.041.60377516
CGTTTAT5950.041.5966381
TGCATTA15850.041.45110315
TTTAGGG48100.041.211023
TTGACCC18850.041.061018
Produced by FastQC (version 0.11.5)